Ligand-induced endocytosis and nuclear localization of angiotensin II receptors expressed in CHO cells
A construct (AT1R-NF) containing a Flag sequence added to the N-terminus of the rat AT1 receptor was stably expressed in Chinese hamster ovary cells and quantified in the cell membrane by confocal microscopy after reaction with a fluorescein-labeled anti-Flag monoclonal antibody. Angiotensin II boun...
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2001 |
| País: | Brasil |
| Institución: | Universidade Federal de São Paulo (UNIFESP) |
| Repositorio: | Repositório Institucional da UNIFESP |
| Idioma: | inglés |
| OAI Identifier: | oai:repositorio.unifesp.br:11600/1236 |
| Acceso en línea: | http://dx.doi.org/10.1590/S0100-879X2001000900011 http://repositorio.unifesp.br/handle/11600/1236 |
| Access Level: | acceso abierto |
| Palabra clave: | angiotensin II agonist-induced receptor internalization endocytosis fluorescence-tagged receptor nuclear angiotensin receptors renin-angiotensin system |
| Sumario: | A construct (AT1R-NF) containing a Flag sequence added to the N-terminus of the rat AT1 receptor was stably expressed in Chinese hamster ovary cells and quantified in the cell membrane by confocal microscopy after reaction with a fluorescein-labeled anti-Flag monoclonal antibody. Angiotensin II bound to AT1R-NF and induced endocytosis with a half-time of 2 min. After 60-90 min, fluorescence accumulated around the cell nucleus, suggesting migration of the ligand-receptor complex to the nuclear membrane. Angiotensin antagonists also induced endocytosis, suggesting that a common step in the transduction signal mechanism occurring after ligand binding may be responsible for the ligand-receptor complex internalization. |
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