Activity of praziquantel on in vitro transformed Schistosoma mansoni sporocysts

Fiocruz-CNPq

Detalles Bibliográficos
Autores: Mattos, Ana Carolina Alves de, Kusel, John Robert, Pimenta, Paulo Filemon Paolucci, Coelho, Paulo Marcos Zach
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2006
País:Brasil
Institución:Fundação Oswaldo Cruz (FIOCRUZ)
Repositorio:Repositório Institucional da FIOCRUZ (ARCA)
Idioma:inglés
OAI Identifier:oai:arca.fiocruz.br:icict/6570
Acceso en línea:https://arca.fiocruz.br/handle/icict/6570
Access Level:acceso abierto
Palabra clave:Praziquantel
Fluorescence
Hoechst 33258
LysoTracker Red
Lectin of Glycine max
Sporocysts
Schistosoma mansoni
id BR_3dd25f45cca2cdee115b2ca4e60e4b5a
oai_identifier_str oai:arca.fiocruz.br:icict/6570
network_acronym_str BR
network_name_str Brasil
repository_id_str
dc.title.none.fl_str_mv Activity of praziquantel on in vitro transformed Schistosoma mansoni sporocysts
title Activity of praziquantel on in vitro transformed Schistosoma mansoni sporocysts
spellingShingle Activity of praziquantel on in vitro transformed Schistosoma mansoni sporocysts
Mattos, Ana Carolina Alves de
Praziquantel
Fluorescence
Hoechst 33258
LysoTracker Red
Lectin of Glycine max
Sporocysts
Schistosoma mansoni
title_short Activity of praziquantel on in vitro transformed Schistosoma mansoni sporocysts
title_full Activity of praziquantel on in vitro transformed Schistosoma mansoni sporocysts
title_fullStr Activity of praziquantel on in vitro transformed Schistosoma mansoni sporocysts
title_full_unstemmed Activity of praziquantel on in vitro transformed Schistosoma mansoni sporocysts
title_sort Activity of praziquantel on in vitro transformed Schistosoma mansoni sporocysts
dc.creator.none.fl_str_mv Mattos, Ana Carolina Alves de
Kusel, John Robert
Pimenta, Paulo Filemon Paolucci
Coelho, Paulo Marcos Zach
author Mattos, Ana Carolina Alves de
author_facet Mattos, Ana Carolina Alves de
Kusel, John Robert
Pimenta, Paulo Filemon Paolucci
Coelho, Paulo Marcos Zach
author_role author
author2 Kusel, John Robert
Pimenta, Paulo Filemon Paolucci
Coelho, Paulo Marcos Zach
author2_role author
author
author
dc.subject.en.none.fl_str_mv Praziquantel
Fluorescence
Hoechst 33258
LysoTracker Red
Lectin of Glycine max
Sporocysts
Schistosoma mansoni
topic Praziquantel
Fluorescence
Hoechst 33258
LysoTracker Red
Lectin of Glycine max
Sporocysts
Schistosoma mansoni
description Fiocruz-CNPq
publishDate 2006
dc.date.issued.fl_str_mv 2006
dc.date.accessioned.fl_str_mv 2013-06-06T14:16:12Z
dc.date.available.fl_str_mv 2013-06-06T14:16:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MATTOS, Ana Carolina Alves de et al. Activity of praziquantel on in vitro transformed Schistosoma mansoni sporocysts. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 101, suppl.1, p. 283-287, 2006.
dc.identifier.uri.fl_str_mv https://arca.fiocruz.br/handle/icict/6570
dc.identifier.issn.none.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02762006000900044
identifier_str_mv MATTOS, Ana Carolina Alves de et al. Activity of praziquantel on in vitro transformed Schistosoma mansoni sporocysts. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 101, suppl.1, p. 283-287, 2006.
0074-0276
10.1590/S0074-02762006000900044
url https://arca.fiocruz.br/handle/icict/6570
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://arca.fiocruz.br/bitstreams/a3770429-311c-4f91-919c-76d3b9272da7/download
https://arca.fiocruz.br/bitstreams/e4581674-f5a3-4b77-96b3-a5607b2c4d6b/download
https://arca.fiocruz.br/bitstreams/7f1885cd-d562-4fb2-87dc-1cc8d389a713/download
https://arca.fiocruz.br/bitstreams/1866a353-2a48-4ffd-9d47-6a0a78f4fd06/download
bitstream.checksum.fl_str_mv 5f9227683c9d83039e74c5484ebe4559
7d48279ffeed55da8dfe2f8e81f3b81f
f3be59b77e4cb46066b354875ef2ec47
d446b66530d6d06db6d7cadc40972698
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1853674480246718464
spelling 2013-06-06T14:16:12Z2013-06-06T14:16:12Z2006MATTOS, Ana Carolina Alves de et al. Activity of praziquantel on in vitro transformed Schistosoma mansoni sporocysts. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 101, suppl.1, p. 283-287, 2006.0074-0276https://arca.fiocruz.br/handle/icict/657010.1590/S0074-02762006000900044Fiocruz-CNPqFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brasil.University of Glasgow. IBLS Division of Infection and Immunity. Scotland, UK.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Entomologia Médica. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brasil / Santa Casa de Misericórdia de Belo Horizonte. Belo Horizonte, MG, Brasil.Praziquantel (PZQ) is effective against all the evolutive phases of Schistosoma mansoni. Infected Biomphalaria glabrata snails have their cercarial shedding interrupted when exposed to PZQ. Using primary in vitro transformed sporocysts, labeled with the probe Hoechst 33258 (indicator of membrane integrity), and lectin of Glycine max (specific for carbohydrate of N-acetylgalactosamine membrane), we evaluated the presence of lysosomes at this evolutive phase of S. mansoni, as well as the influence of PZQ on these acidic organelles and on the tegument of the sporocyst. Although the sporocyst remained alive, it was observed that there was a marked contraction of its musculature, and there occurred a change in the parasite's structure. Also, the acidic vesicles found in the sporo-cysts showed a larger delimited area after contact of the parasites with PZQ. Damages to the tegument was also observed, as show a well-marked labeling either with Hoechst 33258 or with lectin of Glycine max after contact of sporocysts with the drug. These results could partially explain the interruption/reduction mechanism of cercarial shedding in snails exposed to PZQ.engActivity of praziquantel on in vitro transformed Schistosoma mansoni sporocystsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePraziquantelFluorescenceHoechst 33258LysoTracker RedLectin of Glycine maxSporocystsSchistosoma mansoniinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZMattos, Ana Carolina Alves deKusel, John RobertPimenta, Paulo Filemon PaolucciCoelho, Paulo Marcos ZachORIGINAL74.pdf74.pdfapplication/pdf11025228https://arca.fiocruz.br/bitstreams/a3770429-311c-4f91-919c-76d3b9272da7/download5f9227683c9d83039e74c5484ebe4559MD51trueAnonymousREADLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://arca.fiocruz.br/bitstreams/e4581674-f5a3-4b77-96b3-a5607b2c4d6b/download7d48279ffeed55da8dfe2f8e81f3b81fMD52falseAnonymousREADTEXT74.pdf.txt74.pdf.txtExtracted texttext/plain21006https://arca.fiocruz.br/bitstreams/7f1885cd-d562-4fb2-87dc-1cc8d389a713/downloadf3be59b77e4cb46066b354875ef2ec47MD58falseAnonymousREADTHUMBNAIL74.pdf.jpg74.pdf.jpgGenerated Thumbnailimage/jpeg44007https://arca.fiocruz.br/bitstreams/1866a353-2a48-4ffd-9d47-6a0a78f4fd06/downloadd446b66530d6d06db6d7cadc40972698MD59falseAnonymousREADicict/65702025-12-10 14:01:01.335open.accessoai:arca.fiocruz.br:icict/6570https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-12-10T17:01:01Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)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
score 15,300719

Similares en LA Referencia