Serological and molecular detection of Anaplasma phagocytophilum in Thoroughbred horses from Chilean racecourses

Anaplasma phagocytophilum is the causative agent of equine granulocytic anaplasmosis (EGA). This study aimed to perform serological and molecular surveys of A. phagocytophilum in thoroughbred horses from racecourses in Chile. Additionally, hematological findings related to A. phagocytophilum molecul...

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Autores: Hurtado, Claudio, Torres, Reinaldo, Pérez-Macchi, Sandra, Sagredo, Katherine, Uberti, Benjamin, de Souza Zanatto, Diego Carlos [UNESP], Machado, Rosangela Zacarias [UNESP], André, Marcos Rogério [UNESP], Bittencourt, Pedro, Müller, Ananda
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:Brasil
Recursos:Universidade Estadual Paulista (UNESP)
Repositorio:Repositório Institucional da UNESP
Idioma:inglés
OAI Identifier:oai:repositorio.unesp.br:11449/200285
Acesso em linha:http://dx.doi.org/10.1016/j.ttbdis.2020.101441
http://hdl.handle.net/11449/200285
Access Level:acceso abierto
Palavra-chave:Equine granulocytic anaplasmosis
msp2
Serology
South America
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spelling Serological and molecular detection of Anaplasma phagocytophilum in Thoroughbred horses from Chilean racecoursesEquine granulocytic anaplasmosismsp2SerologySouth AmericaAnaplasma phagocytophilum is the causative agent of equine granulocytic anaplasmosis (EGA). This study aimed to perform serological and molecular surveys of A. phagocytophilum in thoroughbred horses from racecourses in Chile. Additionally, hematological findings related to A. phagocytophilum molecular positivity were addressed, and phylogenetic analysis of selected positive samples was performed. Complete blood count and msp2 gene real-time PCR were performed in 457 thoroughbred horses from three racecourses located in three different cities of Chile (Santiago, Viña del Mar and Concepción). Sera from horses in two racecourses (Santiago and Vina del Mar) were tested by Indirect fluorescent antibody test (IFAT) to detect IgG antibodies against A. phagocytophilum. The occurrence of A. phagocytophilum by real-time PCR was 13.6 % (62/457, 95 % CI: 10.8–16.3 %), with the highest occurrence observed in Santiago (26.5 %), followed by Concepción (9%), and the lowest in Viña del Mar (5%). The overall frequency of IgG antibodies to A. phagocytophilum was 7.9 % (23/290, 95 % CI: 4.8–12.7 %), with 9.9 % in Santiago and 6.5 % in Viña del Mar. Only three animals from Santiago Racecourse were positive in both real-time PCR and serology. PCR-positive horses from Santiago racecourse presented significantly lower hemoglobin, mean corpuscular value (MCV), and mean corpuscular hemoglobin concentration (CHCM), and higher eosinophil counts. Phylogenetic analysis based on the msp2 gene showed that A. phagocytophilum sequences found in the present study were closely related with A. phagocytophilum sequences from the USA and Europe. Anaplasma phagocytophilum DNA is detected for the first time in Chile.Instituto de Ciencias Clínicas Veterinarias Facultad de Ciencias Veterinarias Universidad Austral de ChileDepartamento de Patologia Veterinária Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista (FCAV/UNESP)Department of Biomedical Sciences Ross University School of Veterinary MedicineDepartamento de Patologia Veterinária Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista (FCAV/UNESP)Universidad Austral de ChileUniversidade Estadual Paulista (Unesp)Ross University School of Veterinary Medicine2020-12-12T02:02:36Z2020-12-12T02:02:36Z2020-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.ttbdis.2020.101441Ticks and Tick-borne Diseases, v. 11, n. 4, 2020.1877-96031877-959Xhttp://hdl.handle.net/11449/20028510.1016/j.ttbdis.2020.1014412-s2.0-85083344586Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTicks and Tick-borne Diseasesinfo:eu-repo/semantics/openAccessHurtado, ClaudioTorres, ReinaldoPérez-Macchi, SandraSagredo, KatherineUberti, Benjaminde Souza Zanatto, Diego Carlos [UNESP]Machado, Rosangela Zacarias [UNESP]André, Marcos Rogério [UNESP]Bittencourt, PedroMüller, Ananda2024-06-07T13:02:37Zoai:repositorio.unesp.br:11449/200285Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-06-07T13:02:37Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Serological and molecular detection of Anaplasma phagocytophilum in Thoroughbred horses from Chilean racecourses
title Serological and molecular detection of Anaplasma phagocytophilum in Thoroughbred horses from Chilean racecourses
spellingShingle Serological and molecular detection of Anaplasma phagocytophilum in Thoroughbred horses from Chilean racecourses
Hurtado, Claudio
Equine granulocytic anaplasmosis
msp2
Serology
South America
title_short Serological and molecular detection of Anaplasma phagocytophilum in Thoroughbred horses from Chilean racecourses
title_full Serological and molecular detection of Anaplasma phagocytophilum in Thoroughbred horses from Chilean racecourses
title_fullStr Serological and molecular detection of Anaplasma phagocytophilum in Thoroughbred horses from Chilean racecourses
title_full_unstemmed Serological and molecular detection of Anaplasma phagocytophilum in Thoroughbred horses from Chilean racecourses
title_sort Serological and molecular detection of Anaplasma phagocytophilum in Thoroughbred horses from Chilean racecourses
dc.creator.none.fl_str_mv Hurtado, Claudio
Torres, Reinaldo
Pérez-Macchi, Sandra
Sagredo, Katherine
Uberti, Benjamin
de Souza Zanatto, Diego Carlos [UNESP]
Machado, Rosangela Zacarias [UNESP]
André, Marcos Rogério [UNESP]
Bittencourt, Pedro
Müller, Ananda
author Hurtado, Claudio
author_facet Hurtado, Claudio
Torres, Reinaldo
Pérez-Macchi, Sandra
Sagredo, Katherine
Uberti, Benjamin
de Souza Zanatto, Diego Carlos [UNESP]
Machado, Rosangela Zacarias [UNESP]
André, Marcos Rogério [UNESP]
Bittencourt, Pedro
Müller, Ananda
author_role author
author2 Torres, Reinaldo
Pérez-Macchi, Sandra
Sagredo, Katherine
Uberti, Benjamin
de Souza Zanatto, Diego Carlos [UNESP]
Machado, Rosangela Zacarias [UNESP]
André, Marcos Rogério [UNESP]
Bittencourt, Pedro
Müller, Ananda
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Austral de Chile
Universidade Estadual Paulista (Unesp)
Ross University School of Veterinary Medicine
dc.subject.por.fl_str_mv Equine granulocytic anaplasmosis
msp2
Serology
South America
topic Equine granulocytic anaplasmosis
msp2
Serology
South America
description Anaplasma phagocytophilum is the causative agent of equine granulocytic anaplasmosis (EGA). This study aimed to perform serological and molecular surveys of A. phagocytophilum in thoroughbred horses from racecourses in Chile. Additionally, hematological findings related to A. phagocytophilum molecular positivity were addressed, and phylogenetic analysis of selected positive samples was performed. Complete blood count and msp2 gene real-time PCR were performed in 457 thoroughbred horses from three racecourses located in three different cities of Chile (Santiago, Viña del Mar and Concepción). Sera from horses in two racecourses (Santiago and Vina del Mar) were tested by Indirect fluorescent antibody test (IFAT) to detect IgG antibodies against A. phagocytophilum. The occurrence of A. phagocytophilum by real-time PCR was 13.6 % (62/457, 95 % CI: 10.8–16.3 %), with the highest occurrence observed in Santiago (26.5 %), followed by Concepción (9%), and the lowest in Viña del Mar (5%). The overall frequency of IgG antibodies to A. phagocytophilum was 7.9 % (23/290, 95 % CI: 4.8–12.7 %), with 9.9 % in Santiago and 6.5 % in Viña del Mar. Only three animals from Santiago Racecourse were positive in both real-time PCR and serology. PCR-positive horses from Santiago racecourse presented significantly lower hemoglobin, mean corpuscular value (MCV), and mean corpuscular hemoglobin concentration (CHCM), and higher eosinophil counts. Phylogenetic analysis based on the msp2 gene showed that A. phagocytophilum sequences found in the present study were closely related with A. phagocytophilum sequences from the USA and Europe. Anaplasma phagocytophilum DNA is detected for the first time in Chile.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-12T02:02:36Z
2020-12-12T02:02:36Z
2020-07-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.ttbdis.2020.101441
Ticks and Tick-borne Diseases, v. 11, n. 4, 2020.
1877-9603
1877-959X
http://hdl.handle.net/11449/200285
10.1016/j.ttbdis.2020.101441
2-s2.0-85083344586
url http://dx.doi.org/10.1016/j.ttbdis.2020.101441
http://hdl.handle.net/11449/200285
identifier_str_mv Ticks and Tick-borne Diseases, v. 11, n. 4, 2020.
1877-9603
1877-959X
10.1016/j.ttbdis.2020.101441
2-s2.0-85083344586
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Ticks and Tick-borne Diseases
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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score 15,300719

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