Biochemical profile and clinical significance of MAPK/KINASE pathway genes in the diagnosis of thyroid neoplasms
Thyroid neoplasms are the main types of endocrine malignancy, their incidence increasing in recent years. Routine diagnosis may present inconclusive results, leading to the need for using additional techniques that are more precise, such as molecular analysis. Therefore, it is essential to search fo...
| Autores: | , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2022 |
| País: | Brasil |
| Institución: | Universidade Federal de Itajubá (UNIFEI) |
| Repositorio: | Research, Society and Development |
| Idioma: | portugués |
| OAI Identifier: | oai:ojs.pkp.sfu.ca:article/26671 |
| Acceso en línea: | https://rsdjournal.org/index.php/rsd/article/view/26671 |
| Access Level: | acceso abierto |
| Palabra clave: | Thyroid Gene expression Molecular marker MAPK/KINASE Spectroscopy FTIR. Tireoide Expressão gênica Marcador molecular Espectroscopia Tiroides La expresion genica |
| Sumario: | Thyroid neoplasms are the main types of endocrine malignancy, their incidence increasing in recent years. Routine diagnosis may present inconclusive results, leading to the need for using additional techniques that are more precise, such as molecular analysis. Therefore, it is essential to search for molecular markers for the diagnosis of these malignancies and their different histological types. The objective of this study was to identify diagnostic molecular markers for papillary thyroid carcinomas (PTC) and goiter lesions. For this, expression of genes belonging to the MAPK/KINASE pathway was assessed by the RT-qPCR technique. Additionally, complete biochemical profiles of the samples were obtained using Fourier transform infrared spectroscopy (FTIR). Results of RT-qPCR suggest that FOS, JUN, MAP2K6, CCNA1, SFN genes have the potential to be tumor markers of thyroid lesions, and the MAP2K6, CCNA1, SFN genes further have the potential to distinguish PTC samples from other thyroid lesions. FTIR results showed that PTC lesions can be distinguished from normal and benign tissues with 95.83% efficiency; changes in nucleic acids being the major classifying factor. Overall, results suggest potential of molecular and FTIR analysis in diagnosis of thyroid cancer. |
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