Escherichia coli in broiler chickens with airsacculitis

ABSTRACT. Machado L.S., do Nascimento E.R., Pereira V.L.A., Abreu D.L.C., Gouvea R. & Santos L.M.M. 2014. [Escherichia coli in broiler chickens with airsacculitis.] Escherichia coli em frangos de corte com aerossaculite. Revista Brasileira de Medicina Veterinária, 36(3):261-265, 2014. Depart...

Descripción completa

Detalles Bibliográficos
Autores: Machado, Leandro S., do Nascimento, Elmiro Rosendo, Pereira, Virgínia L.A., Abreu, Dayse L.C., Gouvea, Raquel, Santos, Lídia M.M.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2014
País:Brasil
Institución:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)
Repositorio:Brazilian Journal of Veterinary Medicine
Idioma:portugués
OAI Identifier:oai:ojs.rbmv.org:article/523
Acceso en línea:https://bjvm.org.br/BJVM/article/view/523
Access Level:acceso abierto
Palabra clave:Frangos de corte
aerossaculite
micoplasmas
Escherichia coli
PCR
Broilers
airsacculitis
Mycoplasma
Descripción
Sumario:ABSTRACT. Machado L.S., do Nascimento E.R., Pereira V.L.A., Abreu D.L.C., Gouvea R. & Santos L.M.M. 2014. [Escherichia coli in broiler chickens with airsacculitis.] Escherichia coli em frangos de corte com aerossaculite. Revista Brasileira de Medicina Veterinária, 36(3):261-265, 2014. Departamento de Medicina Veterinária Preventiva e Saúde Pública, Faculdade de Veterinária, Universidade Federal Fluminense, Rua Dr. Vital Brazil Filho 64, Vital Brazil, Niterói, RJ 24230-340, Brazil. E-mail: leandromachadovet@yahoo.com.br The Brazilian poultry industry grows each year and becomes increasingly representative in the production and export of products. The health care with poultry have accompanied and favored this evolution, however, respiratory agents that affect the weight and carcass quality, continue to cause great damage to the poultry industry. Airsacculitis is considered the main cause of total and partial condemnation of carcasses of broilers, and has been attributed to Mycoplasmosis mostly caused by Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) and Escherichia coli. The aim of this study was to relate the positivity of MG / MS and E. coli detected by PCR as a risk factor for airsacculitis in condemnation of broilers in Health Inspection Service. We studied 30 broiler poultry slaughtered in a slaughterhouse under Federal Sanitary Inspection, located in the State of Rio de Janeiro. 30 chickens were randomly collected from different lots and tracheas obtained in each PCR. DNA was extracted by phenol-chloroform method and amplified using pairs of “primer”specific for MG, MS and E. coli. Of the 30 chickens analyzed by PCR, 30% (9/30) had lesions in air sacs. None of the birds showed infection with MG and/or MS PCR, however 33.3% (3/9) birds were positive for airsacculitis iss gene from E.coli. E.coli found in broiler chickens that were negative for mycoplasma airsacculitis, implying the presence of such bacteria may be sufficient to cause respiratory problems in broilers and no influence on diagnosis by type of material collection biological.