Protein, oil, and isoflavone contents in lipoxygenase- and Kunitz trypsin inhibitor-deficient soybean seeds

Two traditional soybean cultivars, CAC-1 and Garimpo, two backcrossed-derived isolines lacking lipoxygenase (LOX−KTI+), and two backcrossed-derived isolines lacking lipoxygenase and Kunitz trypsin inhibitor (LOX−KTI−) in the seeds were grown in São Gotardo and Paracatu, Brazil, and their protein, oi...

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Detalles Bibliográficos
Autores: Oliveira, Luiz O., Oliveira, Maria Isaura P., Piovesan, Nilton D., José, Ines C., Barros, Everaldo G., Moreira, Maurilio A.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2007
País:Brasil
Institución:Universidade Federal de Viçosa (UFV)
Repositorio:LOCUS Repositório Institucional da UFV
Idioma:inglés
OAI Identifier:oai:locus.ufv.br:123456789/22166
Acceso en línea:https://doi.org/10.1365/s10337-007-0339-y
http://www.locus.ufv.br/handle/123456789/22166
Access Level:acceso abierto
Palabra clave:Column liquid chromatography
Kunitz–trypsin inhibitor
Isoflavone
Lipoxygenase
Glycine-max
Descripción
Sumario:Two traditional soybean cultivars, CAC-1 and Garimpo, two backcrossed-derived isolines lacking lipoxygenase (LOX−KTI+), and two backcrossed-derived isolines lacking lipoxygenase and Kunitz trypsin inhibitor (LOX−KTI−) in the seeds were grown in São Gotardo and Paracatu, Brazil, and their protein, oil, and isoflavone contents were assessed. The loss of LOX or the combined loss of LOX and KTI brought about no significant changes in seed oil content. Significant decreases in seed protein content were observed in isolines of the CAC-1 genetic background, but not in isolines of the Garimpo background. Total isoflavone contents in LOX−KTI+ and LOX−KTI− isolines were higher than those in the normal parental cultivars. Genetic eliminations also changed isoflavone composition. These results suggest that the genetic removal LOX and KTI may be an efficient approach to improve nutritional and nutraceutical values of soybean seeds, however, performances of null lines should be evaluated for undesirable modifications in seed composition.