Cytokine and nitric oxide patterns in dogs immunized with LBSap vaccine, before and after experimental challenge with Leishmania chagasi plus saliva of Lutzomyia longipalpis.

In the studies presented here, dogs were vaccinated against Leishmania (Leishmania) cha-gasi challenge infection using a preparation of Leishmania braziliensis promastigote proteinsand saponin as adjuvant (LBSap). Vaccination with LBSap induced a prominent type 1immune response that was characterize...

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Detalhes bibliográficos
Autores: Resende, Lucilene Aparecida, Roatt, Bruno Mendes, Soares, Rodrigo Dian de Oliveira Aguiar, Viana, Kelvinson Fernandes, Mendonça, Ludmila Zanandreis de, Lanna, Mariana Ferreira, Lemos, Denise da Silveira, Oliveira, Rodrigo Corrêa de, Martins Filho, Olindo Assis, Fujiwara, Ricardo Toshio, Carneiro, Cláudia Martins, Reis, Alexandre Barbosa, Giunchetti, Rodolfo Cordeiro
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2013
País:Brasil
Recursos:Universidade Federal de Ouro Preto (UFOP)
Repositorio:Repositório Institucional da UFOP
Idioma:inglés
OAI Identifier:oai:repositorio.ufop.br:123456789/4330
Acesso em linha:http://www.repositorio.ufop.br/handle/123456789/4330
https://doi.org/10.1016/j.vetpar.2013.09.011
Access Level:acceso abierto
Palavra-chave:Vaccine
Canine visceral leishmaniasis
Cytokines
immunoprotection
Leishmania chagasi
Descrição
Resumo:In the studies presented here, dogs were vaccinated against Leishmania (Leishmania) cha-gasi challenge infection using a preparation of Leishmania braziliensis promastigote proteinsand saponin as adjuvant (LBSap). Vaccination with LBSap induced a prominent type 1immune response that was characterized by increased levels of interleukin (IL-) 12 andinterferon gamma (IFN- _) production by peripheral blood mononuclear cells (PBMC) uponstimulation with soluble vaccine antigen. Importantly, results showed that this type ofresponsiveness was sustained after challenge infection; at day 90 and 885 after L. chagasichallenge infection, PBMCs from LBSap vaccinated dogs produced more IL-12, IFN- _ andconcomitant nitric oxide (NO) when stimulated with Leishmania antigens as comparedto PBMCs from respective control groups (saponin, LB- treated, or non-treated controldogs). Moreover, transforming growth factor (TGF)- _ decreased in the supernatant of SLcA-stimulated PBMCs in the LBSap group at 90 days. Bone marrow parasitological analysisrevealed decreased frequency of parasitism in the presence of vaccine antigen. It is con-cluded that vaccination of dogs with LBSap vaccine induced a long-lasting type 1 immuneresponse against L. chagasi challenge infection.

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