NANOEMULSÃO DO ÓLEO DO TUCUMÃ (Astrocaryum vulgare Mart.) COMO AGENTE NEUROPROTETOR IN VITRO
Neuropsychiatric diseases have a complex pathophysiology, where some of them are being associated to oxidative stress and mitochondrial dysfunction with consequent exacerbated inflammatory response. In this sense, researches using natural products with bioactive and pharmacological properties are st...
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| Tipo de recurso: | tesis de maestría |
| Estado: | Versión publicada |
| Fecha de publicación: | 2019 |
| País: | Brasil |
| Institución: | Universidade Franciscana (UFN) |
| Repositorio: | Biblioteca Digital de Teses e Dissertações da Universidade Franciscana (UFN) |
| Idioma: | portugués |
| OAI Identifier: | oai:tede.universidadefranciscana.edu.br:UFN-BDTD/788 |
| Acceso en línea: | http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/788 |
| Access Level: | acceso abierto |
| Palabra clave: | Doenças neuropsiquiátricas, Neuroinflamação, Produto natural, Nanotecnologia. Neuropsychiatric diseases, Neuroinflammation, Natural product, Nanotechnology Nanociências |
| Sumario: | Neuropsychiatric diseases have a complex pathophysiology, where some of them are being associated to oxidative stress and mitochondrial dysfunction with consequent exacerbated inflammatory response. In this sense, researches using natural products with bioactive and pharmacological properties are strongly important, as well as the use of nanotechnology to improve bioavailability of these compounds and potentially slow up, prevent and treat these illnesses. In the present study it was prepared nanoemulsions of tucuma oil aiming to protect its chemical matrix and improve its biodistribution. Also it was prepared blank nanoemulsions, without principle. Both formulations were characterized according to mean diameter, polydispersity index, zeta potential, pH and morphology. Formulation stability during 90 days in different stored conditions was also measured. Neuron-like and microglia cell lines (SH-SY5Y and BV-2, respectively) were used to evaluate the safety profile of free tucuma oil and its nanoemulsion. Considering the first results, tucuma oil nanoemulsion was tested regarding to anti-neuroinflammatory response in microglia BV-2 cells activated with lipopolysaccharide. Results related to nanoemulsion production showed nanoemulsion with mean diameter in nanometric scale, small polydispersity index, negative zeta potential, acid pH, and spherical morphology. The best storing condition was under refrigeration. The cytotoxicity evaluation in BV-2 and SH-SY5Y cells it was not observed expressive damages. The anti-neuroinflammatory effect of nanoemulsion of tucuma oil tests showed that although it was not capable to bring cellular proliferation to negative control levels, there was a decresing in this parameter. Also, tucumã nanoemulsion recovered oxidative profile and modulated cellular cycle by G0/G1 cell arresting under 1 μg/mL. In this contexto, we believe that tucuma oil can act as an anti-inflammatory agent and through nanotechnology this effect can be increased, perhaps being able to cross the blood-brain barrier and possibly modulate neuroinflammation associated to neuropsychiatric and neurodegenerative diseases. |
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