Reproductive biology of pêga jackasses: Characteristics of sperm-rich fraction collected using the split-ejaculate method
The aim of this study was to evaluate the physical and morphological characteristics of the sperm-rich fraction of jackass semen. To this end, 130 ejaculates from five Pêga jackasses were collected using an open model artificial vagina. The sperm-rich fraction was collected using the split-ejaculate...
| Authors: | , , , , , , , |
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| Format: | article |
| Status: | Published version |
| Publication Date: | 2016 |
| Country: | Brasil |
| Institution: | Universidade Estadual Paulista (UNESP) |
| Repository: | Repositório Institucional da UNESP |
| Language: | English |
| OAI Identifier: | oai:repositorio.unesp.br:11449/168937 |
| Online Access: | http://dx.doi.org/10.1590/1678-4162-7939 http://hdl.handle.net/11449/168937 |
| Access Level: | Open access |
| Keyword: | Jackass Sperm-rich fraction Split-ejaculate method |
| Summary: | The aim of this study was to evaluate the physical and morphological characteristics of the sperm-rich fraction of jackass semen. To this end, 130 ejaculates from five Pêga jackasses were collected using an open model artificial vagina. The sperm-rich fraction was collected using the split-ejaculate method and assessed for the number of mounts/ejaculate, for physical and morphological characteristics of the semen and number of doses produced/ejaculate. It was observed that all characteristics evaluated differed among the five jackasses, except for the head defect rates. The mean values obtained for the jackass sperm-rich fraction collected were: number of mounts/ejaculate-1.27; semen volume-20.21mL; motility-84.53%; vigor-4.46; motility after dilution-80.10%; sperm concentration/mL-894.38 x 106; total sperm/ejaculate-16.14 x 109; number of insemination doses/ejaculate (400x106 motile sptz)-33.39; number of insemination doses/ejaculate (800 x 106 motile sptz)-16.69; and percentage of normal sperm-90.46%. Thus, in the present experiment the split-ejaculate method using an open artificial vagina worked well with the jackasses, and the sperm-rich fraction of the ejaculate of Pêga jackasses had high quality and sperm concentration, allowing its use for semen processing without reducing the number of insemination doses produced per ejaculate. |
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