Phenotypic Susceptibility to Antiretrovirals Among Clades C, F, and B/F Recombinant Antiretroviral-Naive HIV Type 1 Strains
To evaluate antiretroviral phenotypic susceptibility of wild-type HIV-1 strains circulating in Brazil, samples from antiretroviral-naïve individuals infected with subtypes C (n=16), F (n=9), or B/F (n=7), where reverse transcriptase is B and protease is F, were phenotyped using the AntivirogramTM As...
| Autores: | , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2013 |
| País: | Brasil |
| Institución: | Universidade Federal do Rio Grande (FURG) |
| Repositorio: | Repositório Institucional da FURG (RI FURG) |
| Idioma: | inglés |
| OAI Identifier: | oai:repositorio.furg.br:1/7053 |
| Acceso en línea: | http://repositorio.furg.br/handle/1/7053 |
| Access Level: | acceso abierto |
| Palabra clave: | HIV-1 subtype Phenotypic susceptibility Antiretroviral naïve Brazil |
| Sumario: | To evaluate antiretroviral phenotypic susceptibility of wild-type HIV-1 strains circulating in Brazil, samples from antiretroviral-naïve individuals infected with subtypes C (n=16), F (n=9), or B/F (n=7), where reverse transcriptase is B and protease is F, were phenotyped using the AntivirogramTM Assay (Virco, Mechelen, Belgium). Reduced susceptibility to protease inhibitors (PIs) was observed in one C and three F isolates. None of these samples had any known PI resistance mutations. The Phenotypic Fold Change to one PI was above the biologic cut-off in 3 of 96 (3.1%) of clade F phenotypic determinations and 1 of 96 (1.0%) of C. Phenotypic resistance to at least one nucleoside reverse transcriptase inhibitor (NRTI) was found for two B/F, four C, and three F isolates. The Phenotypic Fold Change in susceptibility to NRTIs was above the cut-off value in 9 of 111 (8.1%) of clade C determinations, as compared to 3 of 63 (4.8%) for clade F and 2 of 49 (4.1%) for clade B. The Phenotypic Fold Change to non-NRTI (NNRTI) was above the cut-off in 7 of 32 (21.9%) of C isolates determinations, whereas none of the F isolates had decrease of susceptibility. Only two of the sixteen C samples had a known NNRTI resistance mutation. The NNRTI Fold Change was above the cut-off value in 3 of 14 (21.4%) of phenotypic determination of Brazilian B/F recombinants, representing clade B reverse transcriptase. NNRTI susceptibility should be better investigated in clade C and B/F recombinants. |
|---|