How deep can ectomycorrhizas go? A case study on Pisolithus down to 4 meters in a Brazilian eucalypt plantation

Despite the strong ecological importance of ectomycorrhizal (ECM) fungi, their vertical distribution remains poorly understood. To our knowledge, ECM structures associated with trees have never been reported in depths below 2 meters. In this study, fine roots and ECM root tips were sampled down to 4...

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Detalhes bibliográficos
Autores: Robin, Agnès, Pradier, Céline, Sanguin, Hervé, Mahé, Frédéric, Lambais, George Rodrigues, de Araujo Pereira, Arthur Prudêncio, Germon, Amandine [UNESP], Santana, Maiele Cintra, Tisseyre, Pierre, Pablo, Anne-Laure, Heuillard, Pauline, Sauvadet, Marie, Bouillet, Jean-Pierre, Andreote, Fernando Dini, Plassard, Claude, de Moraes Gonçalves, José Léonardo, Cardoso, Elke Jurandy Bran Nogueira, Laclau, Jean-Paul, Hinsinger, Philippe, Jourdan, Christophe
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:Brasil
Recursos:Universidade Estadual Paulista (UNESP)
Repositorio:Repositório Institucional da UNESP
Idioma:inglés
OAI Identifier:oai:repositorio.unesp.br:11449/198164
Acesso em linha:http://dx.doi.org/10.1007/s00572-019-00917-y
http://hdl.handle.net/11449/198164
Access Level:acceso abierto
Palavra-chave:Deep fine roots
Diversity
ECM root tips
Eucalyptus grandis
Next-generation sequencing
Tropical forest plantations
Descrição
Resumo:Despite the strong ecological importance of ectomycorrhizal (ECM) fungi, their vertical distribution remains poorly understood. To our knowledge, ECM structures associated with trees have never been reported in depths below 2 meters. In this study, fine roots and ECM root tips were sampled down to 4-m depth during the digging of two independent pits differing by their water availability. A meta-barcoding approach based on Illumina sequencing of internal transcribed spacers (ITS1 and ITS2) was carried out on DNA extracted from root samples (fine roots and ECM root tips separately). ECM fungi dominated the root-associated fungal community, with more than 90% of sequences assigned to the genus Pisolithus. The morphological and barcoding results demonstrated, for the first time, the presence of ECM symbiosis down to 4-m. The molecular diversity of Pisolithus spp. was strongly dependent on depth, with soil pH and soil water content as primary drivers of the Pisolithus spp. structure. Altogether, our results highlight the importance to consider the ECM symbiosis in deep soil layers to improve our understanding of fine roots functioning in tropical soils.

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