Somatic cell count and mastitis pathogen detection in composite and single or duplicate quarter milk samples.

Abstract The most acceptable criteria for diagnosing bovine intramammary infections include results of bacteriological culture and measures of inflammation. Therefore, information on the diagnostic characteristics of the procedures used to identify infected quarters is required. Thus, this study was...

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Autores: SOUZA, F. N., CUNHA, A. F., ROSA, D. L. S. O., BRITO, M. A. V. P. e, GUIMARAES, A. S., MENDONCA, L. C., SOUZA, G. N. de, LAGE, A. P., BLAGITZ, M. G., LIBERA, A. M. M. P. D., HEINEMANN, M. B., CERQUEIRA, M. M. O. P.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:Brasil
Institución:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
Repositorio:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Idioma:inglés
OAI Identifier:oai:www.alice.cnptia.embrapa.br:doc/1063805
Acceso en línea:http://www.alice.cnptia.embrapa.br/alice/handle/doc/1063805
Access Level:acceso abierto
Palabra clave:Intramammary infection
Sensitivity
Specificity
Udder health status
Dairy cow
mastitis
somatic cell count
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spelling Somatic cell count and mastitis pathogen detection in composite and single or duplicate quarter milk samples.Intramammary infectionSensitivitySpecificityUdder health statusDairy cowmastitissomatic cell countAbstract The most acceptable criteria for diagnosing bovine intramammary infections include results of bacteriological culture and measures of inflammation. Therefore, information on the diagnostic characteristics of the procedures used to identify infected quarters is required. Thus, this study was designed to evaluate a set of criteria to classify the infectious status of an udder at the quarter (single and duplicate milk samples) and cow (composite milk sample) levels, and to compare the infectious status with somatic cell counts (SCCs) of the samples. Here, the SCC thresholds determined by receiver operating characteristic curve analysis had a higher Youden index using mammary quarter duplicate milk samples as the gold standard for testing compared with single quarter and composite milk samples, especially for samples for which at least one of the duplicates was microbiologically positive, regardless of the mastitis pathogen isolated. The kappa coefficient for bacteriological results of the single quarter milk samples (single S1 and S2) was 0.85+0.019, indicating that single quarter milk sampling can be useful in mastitis control programs. Therefore, the use of composite milk samples to detect mastitis pathogens may be limited to the detection of major pathogens, given their predictive values. Thus, our findings suggest that the milk SCCs and microbiological examinations, although regarded as the most reliable indicators of ongoing mastitis, should be used in an integrated manner in mastitis control programs. Furthermore, the accuracy of single, duplicate and composite microbiological analyses to diagnosis mastitis should be considered for its implications in mastitis control strategies.Fernando N. Souza, UFMG; Adriano F. Cunha, UFMG; Dalila L. S. O. Rosa, UFMG; MARIA APARECIDA V PAIVA E BRITO, CNPGL; ALESSANDRO DE SA GUIMARAES, CNPGL; LETICIA CALDAS MENDONCA, CNPGL; GUILHERME NUNES DE SOUZA, CNPGL; Andrey P. Lage, UFMG; Maiara G. Blagitz, USP; Alice M. M. P. Della Libera, USP; Marcos B. Heinemann, FMVZ/USP; Mônica M.O.P. Cerqueira, UFMG.2017-02-13T11:11:11Z2017-02-13T11:11:11Z2017-02-1320162017-02-13T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePesquisa Veterinária Brasileira, v. 36, n. 9, p. 811-818, 2016.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1063805enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPASOUZA, F. N.CUNHA, A. F.ROSA, D. L. S. O.BRITO, M. A. V. P. eGUIMARAES, A. S.MENDONCA, L. C.SOUZA, G. N. deLAGE, A. P.BLAGITZ, M. G.LIBERA, A. M. M. P. D.HEINEMANN, M. B.CERQUEIRA, M. M. O. P.2017-08-16T04:10:26Zoai:www.alice.cnptia.embrapa.br:doc/1063805Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T04:10:26Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Somatic cell count and mastitis pathogen detection in composite and single or duplicate quarter milk samples.
title Somatic cell count and mastitis pathogen detection in composite and single or duplicate quarter milk samples.
spellingShingle Somatic cell count and mastitis pathogen detection in composite and single or duplicate quarter milk samples.
SOUZA, F. N.
Intramammary infection
Sensitivity
Specificity
Udder health status
Dairy cow
mastitis
somatic cell count
title_short Somatic cell count and mastitis pathogen detection in composite and single or duplicate quarter milk samples.
title_full Somatic cell count and mastitis pathogen detection in composite and single or duplicate quarter milk samples.
title_fullStr Somatic cell count and mastitis pathogen detection in composite and single or duplicate quarter milk samples.
title_full_unstemmed Somatic cell count and mastitis pathogen detection in composite and single or duplicate quarter milk samples.
title_sort Somatic cell count and mastitis pathogen detection in composite and single or duplicate quarter milk samples.
dc.creator.none.fl_str_mv SOUZA, F. N.
CUNHA, A. F.
ROSA, D. L. S. O.
BRITO, M. A. V. P. e
GUIMARAES, A. S.
MENDONCA, L. C.
SOUZA, G. N. de
LAGE, A. P.
BLAGITZ, M. G.
LIBERA, A. M. M. P. D.
HEINEMANN, M. B.
CERQUEIRA, M. M. O. P.
author SOUZA, F. N.
author_facet SOUZA, F. N.
CUNHA, A. F.
ROSA, D. L. S. O.
BRITO, M. A. V. P. e
GUIMARAES, A. S.
MENDONCA, L. C.
SOUZA, G. N. de
LAGE, A. P.
BLAGITZ, M. G.
LIBERA, A. M. M. P. D.
HEINEMANN, M. B.
CERQUEIRA, M. M. O. P.
author_role author
author2 CUNHA, A. F.
ROSA, D. L. S. O.
BRITO, M. A. V. P. e
GUIMARAES, A. S.
MENDONCA, L. C.
SOUZA, G. N. de
LAGE, A. P.
BLAGITZ, M. G.
LIBERA, A. M. M. P. D.
HEINEMANN, M. B.
CERQUEIRA, M. M. O. P.
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Fernando N. Souza, UFMG; Adriano F. Cunha, UFMG; Dalila L. S. O. Rosa, UFMG; MARIA APARECIDA V PAIVA E BRITO, CNPGL; ALESSANDRO DE SA GUIMARAES, CNPGL; LETICIA CALDAS MENDONCA, CNPGL; GUILHERME NUNES DE SOUZA, CNPGL; Andrey P. Lage, UFMG; Maiara G. Blagitz, USP; Alice M. M. P. Della Libera, USP; Marcos B. Heinemann, FMVZ/USP; Mônica M.O.P. Cerqueira, UFMG.
dc.subject.por.fl_str_mv Intramammary infection
Sensitivity
Specificity
Udder health status
Dairy cow
mastitis
somatic cell count
topic Intramammary infection
Sensitivity
Specificity
Udder health status
Dairy cow
mastitis
somatic cell count
description Abstract The most acceptable criteria for diagnosing bovine intramammary infections include results of bacteriological culture and measures of inflammation. Therefore, information on the diagnostic characteristics of the procedures used to identify infected quarters is required. Thus, this study was designed to evaluate a set of criteria to classify the infectious status of an udder at the quarter (single and duplicate milk samples) and cow (composite milk sample) levels, and to compare the infectious status with somatic cell counts (SCCs) of the samples. Here, the SCC thresholds determined by receiver operating characteristic curve analysis had a higher Youden index using mammary quarter duplicate milk samples as the gold standard for testing compared with single quarter and composite milk samples, especially for samples for which at least one of the duplicates was microbiologically positive, regardless of the mastitis pathogen isolated. The kappa coefficient for bacteriological results of the single quarter milk samples (single S1 and S2) was 0.85+0.019, indicating that single quarter milk sampling can be useful in mastitis control programs. Therefore, the use of composite milk samples to detect mastitis pathogens may be limited to the detection of major pathogens, given their predictive values. Thus, our findings suggest that the milk SCCs and microbiological examinations, although regarded as the most reliable indicators of ongoing mastitis, should be used in an integrated manner in mastitis control programs. Furthermore, the accuracy of single, duplicate and composite microbiological analyses to diagnosis mastitis should be considered for its implications in mastitis control strategies.
publishDate 2016
dc.date.none.fl_str_mv 2016
2017-02-13T11:11:11Z
2017-02-13T11:11:11Z
2017-02-13
2017-02-13T11:11:11Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Pesquisa Veterinária Brasileira, v. 36, n. 9, p. 811-818, 2016.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1063805
identifier_str_mv Pesquisa Veterinária Brasileira, v. 36, n. 9, p. 811-818, 2016.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1063805
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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score 15,301603

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