Catecholamine production along the nephron

The present work proposes an extra neural site of catecholamine production along the nephron. LLC-PK1, MDCK, and mIMCD-3 (proximal and distal tubules and inner medullary collecting duct, respectively) presented the following amine concentrations in the cell homogenates: Norepinephrine = 275 +/- 34,...

Descripción completa

Detalles Bibliográficos
Autores: Di Marco, Giovana Seno [UNIFESP], Vio, Carlos P., Santos, Oscar Fernando Pavão dos [UNIFESP], Schor, Nestor [UNIFESP], Casarini, Dulce Elena [UNIFESP]
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2007
País:Brasil
Institución:Universidade Federal de São Paulo (UNIFESP)
Repositorio:Repositório Institucional da UNIFESP
Idioma:inglés
OAI Identifier:oai:repositorio.unifesp.br:11600/29342
Acceso en línea:http://dx.doi.org/10.1159/000110452
http://repositorio.unifesp.br/handle/11600/29342
Access Level:acceso abierto
Palabra clave:catecholamines
nephron
tyrosine hydroxylase
renal cells
id BR_2a58543f09af1e042d47f2f8f6e2c19c
oai_identifier_str oai:repositorio.unifesp.br:11600/29342
network_acronym_str BR
network_name_str Brasil
repository_id_str
spelling Catecholamine production along the nephroncatecholaminesnephrontyrosine hydroxylaserenal cellsThe present work proposes an extra neural site of catecholamine production along the nephron. LLC-PK1, MDCK, and mIMCD-3 (proximal and distal tubules and inner medullary collecting duct, respectively) presented the following amine concentrations in the cell homogenates: Norepinephrine = 275 +/- 34, 56 +/- 16 and 255 +/- 21; Epinephrine = 161 +/- 20, 83 +/- 17 and 53 +/- 7; and Dopamine = 63 +/- 15, 39 +/- 6 and 36 +/- 7 pg mg cell protein (Means +/- SEM), respectively. the culture medium showed Norepinephrine = 168 +/- 25, 22 +/- 3 and 135 +/- 8; Epinephrine = 32 +/- 6, 152 +/- 17 and 39 +/- 5; and Dopamine = 27 +/- 9, 241 +/- 34 and 26 +/- 5 pg/mg cell protein, respectively. the synthesis enzymes as tyrosine hydroxylase, dopa decarboxylase and dopamine beta-hydroxylase were detected by Western blotting. Biopterin, the enzymatic cofactor of tyrosine hydroxylase, was quantified in the intracellular and medium of mIMCD-3 cells (17 +/- 4 and 24 +/- 3 nmol/mg cell protein, respectively) and in the medium of MDCK cells (19 +/- 4 nmol/mg cell protein). the data confirmed that the proximal tubule is an important source of dopa decarboxilase and Dopamine and epithelial cell along the nephron express the biochemical pathway for catecholamine production. Copyright (c) 2007 S. Karger AG, Basel.Universidade Federal de São Paulo, Escola Paulista Med, Div Nephrol, Dept Med, BR-04023062 São Paulo, BrazilPontificia Univ Catolica Chile, Fac Ciencias Biol, Dept Physiol Sci, Santiago, ChileUniversidade Federal de São Paulo, Escola Paulista Med, Div Nephrol, Dept Med, BR-04023062 São Paulo, BrazilWeb of ScienceKargerUniversidade Federal de São Paulo (UNIFESP)Pontificia Univ Catolica Chile2016-01-24T12:41:42Z2016-01-24T12:41:42Z2007-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion919-924http://dx.doi.org/10.1159/000110452Cellular Physiology and Biochemistry. Basel: Karger, v. 20, n. 6, p. 919-924, 2007.10.1159/0001104521015-8987http://repositorio.unifesp.br/handle/11600/29342WOS:000251098700023ark:/48912/0013000028cprengCellular Physiology and Biochemistryinfo:eu-repo/semantics/openAccesshttp://www.karger.com/Services/RightsPermissionsreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPDi Marco, Giovana Seno [UNIFESP]Vio, Carlos P.Santos, Oscar Fernando Pavão dos [UNIFESP]Schor, Nestor [UNIFESP]Casarini, Dulce Elena [UNIFESP]2016-01-24T10:41:42Zoai:repositorio.unifesp.br:11600/29342Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652016-01-24T10:41:42Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Catecholamine production along the nephron
title Catecholamine production along the nephron
spellingShingle Catecholamine production along the nephron
Di Marco, Giovana Seno [UNIFESP]
catecholamines
nephron
tyrosine hydroxylase
renal cells
title_short Catecholamine production along the nephron
title_full Catecholamine production along the nephron
title_fullStr Catecholamine production along the nephron
title_full_unstemmed Catecholamine production along the nephron
title_sort Catecholamine production along the nephron
dc.creator.none.fl_str_mv Di Marco, Giovana Seno [UNIFESP]
Vio, Carlos P.
Santos, Oscar Fernando Pavão dos [UNIFESP]
Schor, Nestor [UNIFESP]
Casarini, Dulce Elena [UNIFESP]
author Di Marco, Giovana Seno [UNIFESP]
author_facet Di Marco, Giovana Seno [UNIFESP]
Vio, Carlos P.
Santos, Oscar Fernando Pavão dos [UNIFESP]
Schor, Nestor [UNIFESP]
Casarini, Dulce Elena [UNIFESP]
author_role author
author2 Vio, Carlos P.
Santos, Oscar Fernando Pavão dos [UNIFESP]
Schor, Nestor [UNIFESP]
Casarini, Dulce Elena [UNIFESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Pontificia Univ Catolica Chile
dc.subject.por.fl_str_mv catecholamines
nephron
tyrosine hydroxylase
renal cells
topic catecholamines
nephron
tyrosine hydroxylase
renal cells
description The present work proposes an extra neural site of catecholamine production along the nephron. LLC-PK1, MDCK, and mIMCD-3 (proximal and distal tubules and inner medullary collecting duct, respectively) presented the following amine concentrations in the cell homogenates: Norepinephrine = 275 +/- 34, 56 +/- 16 and 255 +/- 21; Epinephrine = 161 +/- 20, 83 +/- 17 and 53 +/- 7; and Dopamine = 63 +/- 15, 39 +/- 6 and 36 +/- 7 pg mg cell protein (Means +/- SEM), respectively. the culture medium showed Norepinephrine = 168 +/- 25, 22 +/- 3 and 135 +/- 8; Epinephrine = 32 +/- 6, 152 +/- 17 and 39 +/- 5; and Dopamine = 27 +/- 9, 241 +/- 34 and 26 +/- 5 pg/mg cell protein, respectively. the synthesis enzymes as tyrosine hydroxylase, dopa decarboxylase and dopamine beta-hydroxylase were detected by Western blotting. Biopterin, the enzymatic cofactor of tyrosine hydroxylase, was quantified in the intracellular and medium of mIMCD-3 cells (17 +/- 4 and 24 +/- 3 nmol/mg cell protein, respectively) and in the medium of MDCK cells (19 +/- 4 nmol/mg cell protein). the data confirmed that the proximal tubule is an important source of dopa decarboxilase and Dopamine and epithelial cell along the nephron express the biochemical pathway for catecholamine production. Copyright (c) 2007 S. Karger AG, Basel.
publishDate 2007
dc.date.none.fl_str_mv 2007-01-01
2016-01-24T12:41:42Z
2016-01-24T12:41:42Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1159/000110452
Cellular Physiology and Biochemistry. Basel: Karger, v. 20, n. 6, p. 919-924, 2007.
10.1159/000110452
1015-8987
http://repositorio.unifesp.br/handle/11600/29342
WOS:000251098700023
dc.identifier.dark.fl_str_mv ark:/48912/0013000028cpr
url http://dx.doi.org/10.1159/000110452
http://repositorio.unifesp.br/handle/11600/29342
identifier_str_mv Cellular Physiology and Biochemistry. Basel: Karger, v. 20, n. 6, p. 919-924, 2007.
10.1159/000110452
1015-8987
WOS:000251098700023
ark:/48912/0013000028cpr
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Cellular Physiology and Biochemistry
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
http://www.karger.com/Services/RightsPermissions
eu_rights_str_mv openAccess
rights_invalid_str_mv http://www.karger.com/Services/RightsPermissions
dc.format.none.fl_str_mv 919-924
dc.publisher.none.fl_str_mv Karger
publisher.none.fl_str_mv Karger
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
_version_ 1853670553218449408
score 15,301603

Similares en LA Referencia