Vimentin is synthesized by mouse vascular trophoblast giant cells from embryonic day 7.5 onwards and is a characteristic factor of these cells

Introduction: A few days after implantation, the embryo grows intensely and trophoblast giant cells (TGC) undergo cell rearrangement, especially of their cytoskeleton. Although we previously showed vimentin in mouse antimesometrial TGC at embryonic days (E) 8.5-10.5, by immunostaining, we did not de...

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Autores: Scherholz, Pedro Luiz Andrade [UNIFESP], Souza, P. Cristina de [UNIFESP], Spadacci-Morena, D. D., Katz, Sima Godosevicius [UNIFESP]
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2013
País:Brasil
Institución:Universidade Federal de São Paulo (UNIFESP)
Repositorio:Repositório Institucional da UNIFESP
Idioma:inglés
OAI Identifier:oai:repositorio.unifesp.br:11600/36447
Acceso en línea:http://dx.doi.org/10.1016/j.placenta.2013.04.003
http://repositorio.unifesp.br/handle/11600/36447
Access Level:acceso abierto
Palabra clave:Embryo
Mice
Placenta
Trophoblast
Vimentin
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spelling Vimentin is synthesized by mouse vascular trophoblast giant cells from embryonic day 7.5 onwards and is a characteristic factor of these cellsEmbryoMicePlacentaTrophoblastVimentinIntroduction: A few days after implantation, the embryo grows intensely and trophoblast giant cells (TGC) undergo cell rearrangement, especially of their cytoskeleton. Although we previously showed vimentin in mouse antimesometrial TGC at embryonic days (E) 8.5-10.5, by immunostaining, we did not demonstrate what is the first embryonic day that TGC synthesize vimentin and whether mouse chorioallantoic placental TGC express vimentin. This is of particular interest because cytokeratin is a marker for TGC in the placenta.Methods: We performed in situ hybridization and immunolocalization, combined with histological and stereological techniques, to study vimentin expression between E6.5 and E12.5 and we investigated Vegf and Flt1/Vegfr1 expression in TGC.Results: Analyses of morphologic parameters of TGC showed that the highest expansion of nuclear and cytoplasmic volumes (p <= 0.05) occurred at E7.5. We detected vimentin expression in TGC from E7.5 onwards; vimentin disappeared as TGC degeneration advanced. Primary and secondary TGC showed intense positive immunostaining for vimentin, Vegf and Flt1/Vegfr1 at E7.5. in the chorioallantoic placenta, parietal TGC (zone of giant cells), spiral artery-associated TGC, maternal blood canal-associated TGC and TGC within the sinusoidal spaces of the labyrinth exhibited an intense immunopositive-reaction for vimentin.Discussion: At E7.5 TGC acquire vimentin, Vegf and Flt1/Vegfr1; at the same time, blood begins to drain from maternal vessels. Vimentin synthesis initiates during a differentiation process of TGC and continues throughout the stage of vascular TGC.Conclusions: We propose that vimentin is a characteristic factor of specialized (vascular) TGC, being a valuable tool for studying pathological pregnancies associated with defects in vascular trophoblasts in mice. (C) 2013 Elsevier B.V. All rights reserved.Universidade Federal de São Paulo, Sch Med, Dept Histol & Struct Biol, BR-04023900 São Paulo, BrazilButantan Inst, Lab Pathophysiol, São Paulo, BrazilUniversidade Federal de São Paulo, Sch Med, Dept Histol & Struct Biol, BR-04023900 São Paulo, BrazilWeb of ScienceCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)W B Saunders Co LtdUniversidade Federal de São Paulo (UNIFESP)Butantan Inst2016-01-24T14:31:54Z2016-01-24T14:31:54Z2013-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion518-525http://dx.doi.org/10.1016/j.placenta.2013.04.003Placenta. London: W B Saunders Co Ltd, v. 34, n. 7, p. 518-525, 2013.10.1016/j.placenta.2013.04.0030143-4004http://repositorio.unifesp.br/handle/11600/36447WOS:000320827100002ark:/48912/00130000244w5engPlacentainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPScherholz, Pedro Luiz Andrade [UNIFESP]Souza, P. Cristina de [UNIFESP]Spadacci-Morena, D. D.Katz, Sima Godosevicius [UNIFESP]2017-01-12T08:23:54Zoai:repositorio.unifesp.br:11600/36447Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652017-01-12T08:23:54Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Vimentin is synthesized by mouse vascular trophoblast giant cells from embryonic day 7.5 onwards and is a characteristic factor of these cells
title Vimentin is synthesized by mouse vascular trophoblast giant cells from embryonic day 7.5 onwards and is a characteristic factor of these cells
spellingShingle Vimentin is synthesized by mouse vascular trophoblast giant cells from embryonic day 7.5 onwards and is a characteristic factor of these cells
Scherholz, Pedro Luiz Andrade [UNIFESP]
Embryo
Mice
Placenta
Trophoblast
Vimentin
title_short Vimentin is synthesized by mouse vascular trophoblast giant cells from embryonic day 7.5 onwards and is a characteristic factor of these cells
title_full Vimentin is synthesized by mouse vascular trophoblast giant cells from embryonic day 7.5 onwards and is a characteristic factor of these cells
title_fullStr Vimentin is synthesized by mouse vascular trophoblast giant cells from embryonic day 7.5 onwards and is a characteristic factor of these cells
title_full_unstemmed Vimentin is synthesized by mouse vascular trophoblast giant cells from embryonic day 7.5 onwards and is a characteristic factor of these cells
title_sort Vimentin is synthesized by mouse vascular trophoblast giant cells from embryonic day 7.5 onwards and is a characteristic factor of these cells
dc.creator.none.fl_str_mv Scherholz, Pedro Luiz Andrade [UNIFESP]
Souza, P. Cristina de [UNIFESP]
Spadacci-Morena, D. D.
Katz, Sima Godosevicius [UNIFESP]
author Scherholz, Pedro Luiz Andrade [UNIFESP]
author_facet Scherholz, Pedro Luiz Andrade [UNIFESP]
Souza, P. Cristina de [UNIFESP]
Spadacci-Morena, D. D.
Katz, Sima Godosevicius [UNIFESP]
author_role author
author2 Souza, P. Cristina de [UNIFESP]
Spadacci-Morena, D. D.
Katz, Sima Godosevicius [UNIFESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Butantan Inst
dc.subject.por.fl_str_mv Embryo
Mice
Placenta
Trophoblast
Vimentin
topic Embryo
Mice
Placenta
Trophoblast
Vimentin
description Introduction: A few days after implantation, the embryo grows intensely and trophoblast giant cells (TGC) undergo cell rearrangement, especially of their cytoskeleton. Although we previously showed vimentin in mouse antimesometrial TGC at embryonic days (E) 8.5-10.5, by immunostaining, we did not demonstrate what is the first embryonic day that TGC synthesize vimentin and whether mouse chorioallantoic placental TGC express vimentin. This is of particular interest because cytokeratin is a marker for TGC in the placenta.Methods: We performed in situ hybridization and immunolocalization, combined with histological and stereological techniques, to study vimentin expression between E6.5 and E12.5 and we investigated Vegf and Flt1/Vegfr1 expression in TGC.Results: Analyses of morphologic parameters of TGC showed that the highest expansion of nuclear and cytoplasmic volumes (p <= 0.05) occurred at E7.5. We detected vimentin expression in TGC from E7.5 onwards; vimentin disappeared as TGC degeneration advanced. Primary and secondary TGC showed intense positive immunostaining for vimentin, Vegf and Flt1/Vegfr1 at E7.5. in the chorioallantoic placenta, parietal TGC (zone of giant cells), spiral artery-associated TGC, maternal blood canal-associated TGC and TGC within the sinusoidal spaces of the labyrinth exhibited an intense immunopositive-reaction for vimentin.Discussion: At E7.5 TGC acquire vimentin, Vegf and Flt1/Vegfr1; at the same time, blood begins to drain from maternal vessels. Vimentin synthesis initiates during a differentiation process of TGC and continues throughout the stage of vascular TGC.Conclusions: We propose that vimentin is a characteristic factor of specialized (vascular) TGC, being a valuable tool for studying pathological pregnancies associated with defects in vascular trophoblasts in mice. (C) 2013 Elsevier B.V. All rights reserved.
publishDate 2013
dc.date.none.fl_str_mv 2013-07-01
2016-01-24T14:31:54Z
2016-01-24T14:31:54Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.placenta.2013.04.003
Placenta. London: W B Saunders Co Ltd, v. 34, n. 7, p. 518-525, 2013.
10.1016/j.placenta.2013.04.003
0143-4004
http://repositorio.unifesp.br/handle/11600/36447
WOS:000320827100002
dc.identifier.dark.fl_str_mv ark:/48912/00130000244w5
url http://dx.doi.org/10.1016/j.placenta.2013.04.003
http://repositorio.unifesp.br/handle/11600/36447
identifier_str_mv Placenta. London: W B Saunders Co Ltd, v. 34, n. 7, p. 518-525, 2013.
10.1016/j.placenta.2013.04.003
0143-4004
WOS:000320827100002
ark:/48912/00130000244w5
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Placenta
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 518-525
dc.publisher.none.fl_str_mv W B Saunders Co Ltd
publisher.none.fl_str_mv W B Saunders Co Ltd
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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score 15,301629

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