Comparison between two techniques of analysis of lung elastic fibers in premature rabbits

Introduction: Bronchopulmonary dysplasia is characterized by a blockage in lung development. Objective: To compare the efficiency of two techniques of analysis of elastic fibers in lungs of premature rabbits. Methods: Preterm rabbits born on the 28th and 29th days of gestation were subjected to ambi...

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Detalhes bibliográficos
Autores: Thereza, Fernanda Polastri, Silva, Mariana Mendes, dos Santos, Rodrigo Leonel, Aquino, Bruno Garcia, Rebello, Celso Moura, Mascaretti, Renata Suman, Manzano, Roberta Munhoz
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2012
País:Brasil
Recursos:Universidade Nove de Julho (UNINOVE)
Repositório:Revista Conscientiae Saúde (Online)
Idioma:português
OAI Identifier:oai:ojs.periodicos.uninove.br:article/3278
Acesso em linha:https://periodicos.uninove.br/saude/article/view/3278
Access Level:Acceso aberto
Palavra-chave:Bronchopulmonary dysplasia
Elastic tissue
Infant
premature.
Displasia broncopulmonar
Tecido elástico
Prematuro.
Descrição
Resumo:Introduction: Bronchopulmonary dysplasia is characterized by a blockage in lung development. Objective: To compare the efficiency of two techniques of analysis of elastic fibers in lungs of premature rabbits. Methods: Preterm rabbits born on the 28th and 29th days of gestation were subjected to ambient air or oxygen for 11 days, randomized into four groups, Ar 28 (n=17), O2 28 (n= 7), Ar 29 (n=8), O2 29 (n=8). The lungs were removed and stained for analysis of elastic fibers. The Nikkon E600 microscope and the image analysis software (Image-Pro Plus 4.5®) were used for elastic fiber analysis. To compare the analysis between microscope and program was used paired “t” test. Results: There were significant differences between the proportion of elastic fibers performed on the microscope and at the program (p