Cubosomes functionalized with antibodies as a potential strategy for the treatment of HER2-positive breast cancer

Breast cancers that overexpress human epidermal growth factor receptor 2 (HER2) have poor prognosis. Moreover, available chemotherapies cause numerous side effects due to poor selectivity. To advance more effective and safer therapies for HER2-positive breast cancer, we explored the fusion of drug d...

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Detalles Bibliográficos
Autores: Victorelli, Francesca Damiani, Lutz-Bueno, Viviane, Santos, Kaio Pini [UNESP], Wu, Di, Sturla, Shana J., Mezzenga, Raffaele
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:Brasil
Institución:Universidade Estadual Paulista (UNESP)
Repositorio:Repositório Institucional da UNESP
Idioma:inglés
OAI Identifier:oai:repositorio.unesp.br:11449/301007
Acceso en línea:http://dx.doi.org/10.1016/j.jcis.2024.06.091
https://hdl.handle.net/11449/301007
Access Level:acceso abierto
Palabra clave:Breast cancer
Cubosomes
Drug delivery
Monoclonal antibody
Panobinostat
Targeting
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spelling Cubosomes functionalized with antibodies as a potential strategy for the treatment of HER2-positive breast cancerBreast cancerCubosomesDrug deliveryMonoclonal antibodyPanobinostatTargetingBreast cancers that overexpress human epidermal growth factor receptor 2 (HER2) have poor prognosis. Moreover, available chemotherapies cause numerous side effects due to poor selectivity. To advance more effective and safer therapies for HER2-positive breast cancer, we explored the fusion of drug delivery technology and immunotherapy. Our research led to the design of immunocubosomes loaded with panobinostat and functionalized with trastuzumab antibodies, enabling precise targeting of breast cancer cells that overexpress HER2. We characterised the nanostructure of cubosomes using small-angle X-ray scattering (SAXS), cryo-transmission electron microscopy (cryo-TEM), and dynamic light scattering (DLS). Moreover, we confirmed the integrity of the trastuzumab antibodies on the immunocubosomes by Fourier-transform infrared spectroscopy (FTIR) and sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Additionally, we found that panobinostat-loaded immunocubosomes were more cytotoxic, and in an uptake-dependant manner, towards a HER2-positive breast cancer cell line (SKBR3) compared to a cell line representing healthy cells (L929). These results support that the functionalization of cubosomes with antibodies enhances both the effectiveness of the loaded drug and its selectivity for targeting HER2-positive breast cancer cells.Department of Health Sciences and Technology ETH ZurichPaul Scherrer Institute (PSI)Department of Drugs and Medicine School of Pharmaceutical Sciences São Paulo State University, São PauloCollege of Food Science and Engineering Qingdao Agricultural University, ShandongDepartment of Materials ETH ZurichDepartment of Drugs and Medicine School of Pharmaceutical Sciences São Paulo State University, São PauloETH ZurichPaul Scherrer Institute (PSI)Universidade Estadual Paulista (UNESP)Qingdao Agricultural University2025-04-29T18:56:56Z2024-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article291-300http://dx.doi.org/10.1016/j.jcis.2024.06.091Journal of Colloid and Interface Science, v. 673, p. 291-300.1095-71030021-9797https://hdl.handle.net/11449/30100710.1016/j.jcis.2024.06.0912-s2.0-85195791936Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Colloid and Interface Scienceinfo:eu-repo/semantics/openAccessVictorelli, Francesca DamianiLutz-Bueno, VivianeSantos, Kaio Pini [UNESP]Wu, DiSturla, Shana J.Mezzenga, Raffaele2025-05-01T05:20:50Zoai:repositorio.unesp.br:11449/301007Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462025-05-01T05:20:50Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Cubosomes functionalized with antibodies as a potential strategy for the treatment of HER2-positive breast cancer
title Cubosomes functionalized with antibodies as a potential strategy for the treatment of HER2-positive breast cancer
spellingShingle Cubosomes functionalized with antibodies as a potential strategy for the treatment of HER2-positive breast cancer
Victorelli, Francesca Damiani
Breast cancer
Cubosomes
Drug delivery
Monoclonal antibody
Panobinostat
Targeting
title_short Cubosomes functionalized with antibodies as a potential strategy for the treatment of HER2-positive breast cancer
title_full Cubosomes functionalized with antibodies as a potential strategy for the treatment of HER2-positive breast cancer
title_fullStr Cubosomes functionalized with antibodies as a potential strategy for the treatment of HER2-positive breast cancer
title_full_unstemmed Cubosomes functionalized with antibodies as a potential strategy for the treatment of HER2-positive breast cancer
title_sort Cubosomes functionalized with antibodies as a potential strategy for the treatment of HER2-positive breast cancer
dc.creator.none.fl_str_mv Victorelli, Francesca Damiani
Lutz-Bueno, Viviane
Santos, Kaio Pini [UNESP]
Wu, Di
Sturla, Shana J.
Mezzenga, Raffaele
author Victorelli, Francesca Damiani
author_facet Victorelli, Francesca Damiani
Lutz-Bueno, Viviane
Santos, Kaio Pini [UNESP]
Wu, Di
Sturla, Shana J.
Mezzenga, Raffaele
author_role author
author2 Lutz-Bueno, Viviane
Santos, Kaio Pini [UNESP]
Wu, Di
Sturla, Shana J.
Mezzenga, Raffaele
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv ETH Zurich
Paul Scherrer Institute (PSI)
Universidade Estadual Paulista (UNESP)
Qingdao Agricultural University
dc.subject.por.fl_str_mv Breast cancer
Cubosomes
Drug delivery
Monoclonal antibody
Panobinostat
Targeting
topic Breast cancer
Cubosomes
Drug delivery
Monoclonal antibody
Panobinostat
Targeting
description Breast cancers that overexpress human epidermal growth factor receptor 2 (HER2) have poor prognosis. Moreover, available chemotherapies cause numerous side effects due to poor selectivity. To advance more effective and safer therapies for HER2-positive breast cancer, we explored the fusion of drug delivery technology and immunotherapy. Our research led to the design of immunocubosomes loaded with panobinostat and functionalized with trastuzumab antibodies, enabling precise targeting of breast cancer cells that overexpress HER2. We characterised the nanostructure of cubosomes using small-angle X-ray scattering (SAXS), cryo-transmission electron microscopy (cryo-TEM), and dynamic light scattering (DLS). Moreover, we confirmed the integrity of the trastuzumab antibodies on the immunocubosomes by Fourier-transform infrared spectroscopy (FTIR) and sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Additionally, we found that panobinostat-loaded immunocubosomes were more cytotoxic, and in an uptake-dependant manner, towards a HER2-positive breast cancer cell line (SKBR3) compared to a cell line representing healthy cells (L929). These results support that the functionalization of cubosomes with antibodies enhances both the effectiveness of the loaded drug and its selectivity for targeting HER2-positive breast cancer cells.
publishDate 2024
dc.date.none.fl_str_mv 2024-11-01
2025-04-29T18:56:56Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.jcis.2024.06.091
Journal of Colloid and Interface Science, v. 673, p. 291-300.
1095-7103
0021-9797
https://hdl.handle.net/11449/301007
10.1016/j.jcis.2024.06.091
2-s2.0-85195791936
url http://dx.doi.org/10.1016/j.jcis.2024.06.091
https://hdl.handle.net/11449/301007
identifier_str_mv Journal of Colloid and Interface Science, v. 673, p. 291-300.
1095-7103
0021-9797
10.1016/j.jcis.2024.06.091
2-s2.0-85195791936
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Colloid and Interface Science
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 291-300
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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score 15,300719

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