Application of a quantitative HPLC-ESI-MS/MS method for flavonoids in different vegetable matrices

Plant flavonoids have been increasingly studied for its importance in plant metabolism, inter-species interactions and human health-promoting effects. Each botanical species has a distinct flavonoid profile, and as such, this work aimed to develop and apply a high-performance liquid chromatography c...

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Detalhes bibliográficos
Autores: Perlatti, Bruno, Fernandes, João B., Silva, Maria F. G. F., Ardila, Jorge A., Carneiro, Renato L., Souza, Bruno H. S., Costa, Eduardo N., Eduardo, Wellington I., Boiça Junior, Arlindo L., Forim, Moacir R.
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:Brasil
Recursos:Universidade Federal de Lavras (UFLA)
Repositorio:Repositório Institucional da UFLA
Idioma:portugués
OAI Identifier:oai:repositorio.ufla.br:1/30202
Acesso em linha:https://repositorio.ufla.br/handle/1/30202
Access Level:acceso abierto
Palavra-chave:Flavonoid
Quantitative analysis
High performance liquid chromatography - electrospray tandem mass spectrometry (HPLC-ESI-MS-MS)
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repository_id_str
dc.title.pt_BR.fl_str_mv Application of a quantitative HPLC-ESI-MS/MS method for flavonoids in different vegetable matrices
title Application of a quantitative HPLC-ESI-MS/MS method for flavonoids in different vegetable matrices
spellingShingle Application of a quantitative HPLC-ESI-MS/MS method for flavonoids in different vegetable matrices
Perlatti, Bruno
Flavonoid
Quantitative analysis
High performance liquid chromatography - electrospray tandem mass spectrometry (HPLC-ESI-MS-MS)
title_short Application of a quantitative HPLC-ESI-MS/MS method for flavonoids in different vegetable matrices
title_full Application of a quantitative HPLC-ESI-MS/MS method for flavonoids in different vegetable matrices
title_fullStr Application of a quantitative HPLC-ESI-MS/MS method for flavonoids in different vegetable matrices
title_full_unstemmed Application of a quantitative HPLC-ESI-MS/MS method for flavonoids in different vegetable matrices
title_sort Application of a quantitative HPLC-ESI-MS/MS method for flavonoids in different vegetable matrices
dc.creator.none.fl_str_mv Perlatti, Bruno
Fernandes, João B.
Silva, Maria F. G. F.
Ardila, Jorge A.
Carneiro, Renato L.
Souza, Bruno H. S.
Costa, Eduardo N.
Eduardo, Wellington I.
Boiça Junior, Arlindo L.
Forim, Moacir R.
author Perlatti, Bruno
author_facet Perlatti, Bruno
Fernandes, João B.
Silva, Maria F. G. F.
Ardila, Jorge A.
Carneiro, Renato L.
Souza, Bruno H. S.
Costa, Eduardo N.
Eduardo, Wellington I.
Boiça Junior, Arlindo L.
Forim, Moacir R.
author_role author
author2 Fernandes, João B.
Silva, Maria F. G. F.
Ardila, Jorge A.
Carneiro, Renato L.
Souza, Bruno H. S.
Costa, Eduardo N.
Eduardo, Wellington I.
Boiça Junior, Arlindo L.
Forim, Moacir R.
author2_role author
author
author
author
author
author
author
author
author
dc.subject.por.fl_str_mv Flavonoid
Quantitative analysis
High performance liquid chromatography - electrospray tandem mass spectrometry (HPLC-ESI-MS-MS)
topic Flavonoid
Quantitative analysis
High performance liquid chromatography - electrospray tandem mass spectrometry (HPLC-ESI-MS-MS)
description Plant flavonoids have been increasingly studied for its importance in plant metabolism, inter-species interactions and human health-promoting effects. Each botanical species has a distinct flavonoid profile, and as such, this work aimed to develop and apply a high-performance liquid chromatography coupled to electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) method to quantify specific flavonoids in different plants and parts. Six flavonoids (isoquercitrin, quercetin, naringin, naringenin, hesperidin and rutin), two isoflavonoids (daidzin and daidzein) and one coumarin (umbelliferone) were targeted. Calibration curves showed good correlations (R2 > 0.994) and limits of quantification (≤ 500 ng mL-1). The method was applied for detection of analytes in common bean seeds (Phaseolus vulgaris), soybean leaves (Glycine max), and sour orange (Citrus aurantium), sweet orange (Citrus sinensis), and Tahiti lime (Citrus latifolia) flavedo after ultrasound assisted acidic hydroalcoholic extraction. The method was tuned for quick and efficient extraction. Principal component analysis was helpful for the discrimination of matrices based on their flavonoid profiles.
publishDate 2016
dc.date.issued.fl_str_mv 2016-03
dc.date.accessioned.fl_str_mv 2018-08-27T19:31:48Z
dc.date.available.fl_str_mv 2018-08-27T19:31:48Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PERLATTI, B. et al. Application of a quantitative HPLC-ESI-MS/MS method for flavonoids in different vegetable matrices. Journal of the Brazilian Chemical Society, São Paulo, v. 27, n. 3, Mar. 2016.
dc.identifier.uri.fl_str_mv https://repositorio.ufla.br/handle/1/30202
identifier_str_mv PERLATTI, B. et al. Application of a quantitative HPLC-ESI-MS/MS method for flavonoids in different vegetable matrices. Journal of the Brazilian Chemical Society, São Paulo, v. 27, n. 3, Mar. 2016.
url https://repositorio.ufla.br/handle/1/30202
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.pt_BR.fl_str_mv Journal of the Brazilian Chemical Society
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFLA
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institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
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spelling 2018-08-27T19:31:48Z2018-08-27T19:31:48Z2016-03PERLATTI, B. et al. Application of a quantitative HPLC-ESI-MS/MS method for flavonoids in different vegetable matrices. Journal of the Brazilian Chemical Society, São Paulo, v. 27, n. 3, Mar. 2016.https://repositorio.ufla.br/handle/1/30202Plant flavonoids have been increasingly studied for its importance in plant metabolism, inter-species interactions and human health-promoting effects. Each botanical species has a distinct flavonoid profile, and as such, this work aimed to develop and apply a high-performance liquid chromatography coupled to electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) method to quantify specific flavonoids in different plants and parts. Six flavonoids (isoquercitrin, quercetin, naringin, naringenin, hesperidin and rutin), two isoflavonoids (daidzin and daidzein) and one coumarin (umbelliferone) were targeted. Calibration curves showed good correlations (R2 > 0.994) and limits of quantification (≤ 500 ng mL-1). The method was applied for detection of analytes in common bean seeds (Phaseolus vulgaris), soybean leaves (Glycine max), and sour orange (Citrus aurantium), sweet orange (Citrus sinensis), and Tahiti lime (Citrus latifolia) flavedo after ultrasound assisted acidic hydroalcoholic extraction. The method was tuned for quick and efficient extraction. Principal component analysis was helpful for the discrimination of matrices based on their flavonoid profiles.Sociedade Brasileira de QuímicaJournal of the Brazilian Chemical Societyreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAFlavonoidQuantitative analysisHigh performance liquid chromatography - electrospray tandem mass spectrometry (HPLC-ESI-MS-MS)Application of a quantitative HPLC-ESI-MS/MS method for flavonoids in different vegetable matricesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessporPerlatti, BrunoFernandes, João B.Silva, Maria F. G. F.Ardila, Jorge A.Carneiro, Renato L.Souza, Bruno H. S.Costa, Eduardo N.Eduardo, Wellington I.Boiça Junior, Arlindo L.Forim, Moacir R.ORIGINALARTIGO_Application of a quantitative HPLC-ESI-MS-MS method for flavonoids in different vegetable matrices.pdfARTIGO_Application of a quantitative HPLC-ESI-MS-MS method for flavonoids in different vegetable matrices.pdfapplication/pdf584068https://repositorio.ufla.br/bitstreams/b6beb223-2ff1-43f8-b206-39a794ba687a/download3ef3dbc5220448e60ddb85815f69e851MD51trueAnonymousREADLICENSElicense.txtlicense.txttext/plain; charset=utf-8953https://repositorio.ufla.br/bitstreams/633ec347-0ba1-4688-8a0b-4ab04a7eb52c/download760884c1e72224de569e74f79eb87ce3MD52falseAnonymousREAD1/302022025-07-17 11:02:23.185open.accessoai:repositorio.ufla.br:1/30202https://repositorio.ufla.brRepositório InstitucionalPUBhttps://repositorio.ufla.br/server/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2025-07-17T14:02:23Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)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