Avaliação da reação em cadeia da polimerase e do isolamento bacteriológico convencional na detecção de Salmonella Dublin em amostras de fezes de bezerros infectados experimentalmente

The efficiency of the Polymerase Chain Reaction (PCR) combined with selective enrichment broth was compared with the standard microbiological techniques for detection of Salmonella Dublin in fecal samples of 10 to 15-days-old Holstein calves, experimentally infected with 10(8) CFU of Salmonella Dubl...

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Bibliographic Details
Authors: Silva, D. G. [UNESP], Silva, D. R. [UNESP], Silva, P. R. L., Cícero, E. A. S. [UNESP], Ferraz, A.L.J. [UNESP], Lemos, M. V. F. [UNESP], Fagliari, J. J. [UNESP]
Format: article
Status:Published version
Publication Date:2010
Country:Brasil
Institution:Universidade Estadual Paulista (UNESP)
Repository:Repositório Institucional da UNESP
Language:Portuguese
OAI Identifier:oai:repositorio.unesp.br:11449/1691
Online Access:http://dx.doi.org/10.1590/S0102-09352010000300035
http://hdl.handle.net/11449/1691
Access Level:Open access
Keyword:Calves
feces
Salmonella Dublin
PCR
culture
Description
Summary:The efficiency of the Polymerase Chain Reaction (PCR) combined with selective enrichment broth was compared with the standard microbiological techniques for detection of Salmonella Dublin in fecal samples of 10 to 15-days-old Holstein calves, experimentally infected with 10(8) CFU of Salmonella Dublin. Seventy-six fecal samples were analyzed using PCR associated with selenite cystine (SC) and Muller-Kauffmann tetrathionate (TMK) broths and standard microbiological techniques. Regardless of the selective enrichment broth used, the standard microbiological techniques were significantly better than PCR in detection of positive samples of Salmonella Dublin. However, the simultaneous use of both techniques provided detection of a larger number of positive samples. The SC broth was the best option as selective enrichment in both techniques.

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