Terpinen-4-ol and carvacrol affect multi-species biofilm composition

The aim of this study was to investigate the cytotoxic activity and inhibitory effect of terpinen-4-ol (T4ol) and carvacrol against single- and multi-species biofilms. The toxicity of each compound was tested on oral keratinocytes and evaluated by XTT assay. Inhibition and eradication of single-spec...

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Detalles Bibliográficos
Autores: Maquera Huacho, Patricia Milagros [UNESP], Rodriguez Herrero, Esteban, Verspecht, Tim, Pauwels, Martine, Marcantonio, Elcio [UNESP], Palomari Spolidorio, Denise Madalena [UNESP], Teughels, Wim
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:Brasil
Institución:Universidade Estadual Paulista (UNESP)
Repositorio:Repositório Institucional da UNESP
Idioma:inglés
OAI Identifier:oai:repositorio.unesp.br:11449/190473
Acceso en línea:http://dx.doi.org/10.1080/08927014.2019.1630386
http://hdl.handle.net/11449/190473
Access Level:acceso abierto
Palabra clave:carvacrol
multi-species biofilm
peri-implantitis
periodontal disease
Terpinen-4-ol
Descripción
Sumario:The aim of this study was to investigate the cytotoxic activity and inhibitory effect of terpinen-4-ol (T4ol) and carvacrol against single- and multi-species biofilms. The toxicity of each compound was tested on oral keratinocytes and evaluated by XTT assay. Inhibition and eradication of single-species biofilms were analyzed by crystal violet assay and the effect on multi-species biofilm composition was evaluated by qPCR. T4ol and carvacrol did not affect the epithelial cell viability, in contrast to chlorhexidine, which showed a high cytotoxic effect. Inhibition and eradication of single-species biofilms treated with T4ol and carvacrol were observed. The same inhibitory effect was observed for multi-species biofilms, especially on periodontal pathogens. In conclusion, specific concentrations of T4ol and carvacrol without toxicity towards the epithelial cells reduced the numbers of periodontal pathogens in single- and multi-species biofilms.