Bioanalytical method for the quantification of the monastrol derivative anticancer candidate lasom 65 in pre-clinical pharmacokinetic investigations

A simple HPLC/UV method was developed for the determination of the anticancer candidate LaSOM 65 in rat plasma. Samples were cleaned by protein precipitation with acetonitrile (recovery > 95%), after which they were subjected to chromatography under the isocratic elution of an acetonitrile:water...

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Autores: Torres, Bruna Gaelzer Silva, Uchôa, Flávia de Toni, Canto, Rômulo Faria Santos, Crestani, Allan, Eifler-Lima, Vera Lucia, Dalla Costa, Teresa Cristina Tavares
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2014
País:Brasil
Institución:Universidade Federal do Rio Grande do Sul (UFRGS)
Repositorio:Repositório Institucional da UFRGS
Idioma:portugués
OAI Identifier:oai:www.lume.ufrgs.br:10183/115510
Acceso en línea:http://hdl.handle.net/10183/115510
Access Level:acceso abierto
Palabra clave:Monastrol
Farmacocinética
Antineoplásicos
LaSOM 65
HPLC/UV
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spelling 2015-04-23T01:58:58Z20140100-4042http://hdl.handle.net/10183/115510000940410A simple HPLC/UV method was developed for the determination of the anticancer candidate LaSOM 65 in rat plasma. Samples were cleaned by protein precipitation with acetonitrile (recovery > 95%), after which they were subjected to chromatography under the isocratic elution of an acetonitrile:water (45:55, v/v) solution with detection at 303 nm. The method was linear (r2 > 0.98) over the concentration range (0.05–2 μg mL−1) with intra- and inter-day precision ranging from 9.6% to 13.6% and 4.3% to 5.4%, respectively. The accuracy of the method ranged from 85% to 113.6%, and it showed sufficient sensitivity to determine pharmacokinetic parameters of LaSOM 65 after intravenous administration to Wistar rats.application/pdfporQuímica nova. São Paulo. Vol. 37, n. 3 (2014), p. 461-464MonastrolFarmacocinéticaAntineoplásicosLaSOM 65HPLC/UVBioanalytical method for the quantification of the monastrol derivative anticancer candidate lasom 65 in pre-clinical pharmacokinetic investigationsinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTorres, Bruna Gaelzer SilvaUchôa, Flávia de ToniCanto, Rômulo Faria SantosCrestani, AllanEifler-Lima, Vera LuciaDalla Costa, Teresa Cristina TavaresORIGINAL000940410.pdf000940410.pdfTexto completo (inglês)application/pdf253215http://www.lume.ufrgs.br/bitstream/10183/115510/1/000940410.pdf9543c087700e1ecd3494dffcf1e8d8d9MD51TEXT000940410.pdf.txt000940410.pdf.txtExtracted Texttext/plain21105http://www.lume.ufrgs.br/bitstream/10183/115510/2/000940410.pdf.txtba6a54fc61ad38a9ba149cec80b10997MD52THUMBNAIL000940410.pdf.jpg000940410.pdf.jpgGenerated Thumbnailimage/jpeg1869http://www.lume.ufrgs.br/bitstream/10183/115510/3/000940410.pdf.jpg482b2526acdf4cb2e1bb8f2c8c4168a9MD5310183/1155102018-10-19 10:55:27.46oai:www.lume.ufrgs.br:10183/115510Repositório InstitucionalPUBhttps://lume.ufrgs.br/oai/requestlume@ufrgs.bropendoar:2018-10-19T13:55:27Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Bioanalytical method for the quantification of the monastrol derivative anticancer candidate lasom 65 in pre-clinical pharmacokinetic investigations
title Bioanalytical method for the quantification of the monastrol derivative anticancer candidate lasom 65 in pre-clinical pharmacokinetic investigations
spellingShingle Bioanalytical method for the quantification of the monastrol derivative anticancer candidate lasom 65 in pre-clinical pharmacokinetic investigations
Torres, Bruna Gaelzer Silva
Monastrol
Farmacocinética
Antineoplásicos
LaSOM 65
HPLC/UV
title_short Bioanalytical method for the quantification of the monastrol derivative anticancer candidate lasom 65 in pre-clinical pharmacokinetic investigations
title_full Bioanalytical method for the quantification of the monastrol derivative anticancer candidate lasom 65 in pre-clinical pharmacokinetic investigations
title_fullStr Bioanalytical method for the quantification of the monastrol derivative anticancer candidate lasom 65 in pre-clinical pharmacokinetic investigations
title_full_unstemmed Bioanalytical method for the quantification of the monastrol derivative anticancer candidate lasom 65 in pre-clinical pharmacokinetic investigations
title_sort Bioanalytical method for the quantification of the monastrol derivative anticancer candidate lasom 65 in pre-clinical pharmacokinetic investigations
dc.creator.none.fl_str_mv Torres, Bruna Gaelzer Silva
Uchôa, Flávia de Toni
Canto, Rômulo Faria Santos
Crestani, Allan
Eifler-Lima, Vera Lucia
Dalla Costa, Teresa Cristina Tavares
author Torres, Bruna Gaelzer Silva
author_facet Torres, Bruna Gaelzer Silva
Uchôa, Flávia de Toni
Canto, Rômulo Faria Santos
Crestani, Allan
Eifler-Lima, Vera Lucia
Dalla Costa, Teresa Cristina Tavares
author_role author
author2 Uchôa, Flávia de Toni
Canto, Rômulo Faria Santos
Crestani, Allan
Eifler-Lima, Vera Lucia
Dalla Costa, Teresa Cristina Tavares
author2_role author
author
author
author
author
dc.subject.por.fl_str_mv Monastrol
Farmacocinética
Antineoplásicos
topic Monastrol
Farmacocinética
Antineoplásicos
LaSOM 65
HPLC/UV
dc.subject.eng.fl_str_mv LaSOM 65
HPLC/UV
description A simple HPLC/UV method was developed for the determination of the anticancer candidate LaSOM 65 in rat plasma. Samples were cleaned by protein precipitation with acetonitrile (recovery > 95%), after which they were subjected to chromatography under the isocratic elution of an acetonitrile:water (45:55, v/v) solution with detection at 303 nm. The method was linear (r2 > 0.98) over the concentration range (0.05–2 μg mL−1) with intra- and inter-day precision ranging from 9.6% to 13.6% and 4.3% to 5.4%, respectively. The accuracy of the method ranged from 85% to 113.6%, and it showed sufficient sensitivity to determine pharmacokinetic parameters of LaSOM 65 after intravenous administration to Wistar rats.
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2015-04-23T01:58:58Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10183/115510
dc.identifier.issn.pt_BR.fl_str_mv 0100-4042
dc.identifier.nrb.pt_BR.fl_str_mv 000940410
identifier_str_mv 0100-4042
000940410
url http://hdl.handle.net/10183/115510
dc.language.iso.fl_str_mv por
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dc.relation.ispartof.pt_BR.fl_str_mv Química nova. São Paulo. Vol. 37, n. 3 (2014), p. 461-464
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFRGS
instname:Universidade Federal do Rio Grande do Sul (UFRGS)
instacron:UFRGS
instname_str Universidade Federal do Rio Grande do Sul (UFRGS)
instacron_str UFRGS
institution UFRGS
reponame_str Repositório Institucional da UFRGS
collection Repositório Institucional da UFRGS
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