Efficacy of chemo-mechanical preparation with different substances and the use of a root canal medication in dog's teeth with induced periapical lesion

Objectives: to evaluate the effect of instrumentation, irrigation with different substances and the use of calcium hydroxide on bacterial load and microbiota profile in dog's teeth with pulp necrosis and periapical lesion. Methods: Fifty five root canals were divided into groups: I) Saline (SSL...

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Detalles Bibliográficos
Autores: Martinho, Frederico C., Cintra, Luciano Tavares Angelo [UNESP], Zaia, Alexandre A., Ferraz, Caio C.R., Almeida, José F.A., Gomes, Brenda P.F.A.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2011
País:Brasil
Institución:Universidade Estadual Paulista (UNESP)
Repositorio:Repositório Institucional da UNESP
Idioma:inglés
OAI Identifier:oai:repositorio.unesp.br:11449/227930
Acceso en línea:http://dx.doi.org/10.14436/2178-3713.1.1.037-045.oar
http://hdl.handle.net/11449/227930
Access Level:acceso abierto
Palabra clave:Calcium hydroxide
Chlorhexidine
Endodontic infection
Root canal medication
Sodium hypochlorite
Descripción
Sumario:Objectives: to evaluate the effect of instrumentation, irrigation with different substances and the use of calcium hydroxide on bacterial load and microbiota profile in dog's teeth with pulp necrosis and periapical lesion. Methods: Fifty five root canals were divided into groups: I) Saline (SSL) (n=11); II) natrosol gel (n=11); III) 2.5% NaOCl (n=11); IV) 2% CHX-gel (n=11); V) 2% CHX-solution (n=11). Endodontic samples were cultured, microorganisms counted and the microbiota analyzed at different sampling times - s1, s2 and s3. Results: At s1, the mean CFU counts ranged from 5.5 ×105 to 1.5 × 106. These values dropped significantly at s2 (p<0.05). No statistical significant difference was found between s2 and s3. Changes in root canal microbiota were found at s2 and s3. Conclusion: Regardless the use of calcium hydroxide as a root canal medication, 2.5% NaOCl and 2% CHX-gel demonstrated a potent antimicrobial activity against endododontic pathogens in vivo.