In vitro inhibition of acetylcholinesterase and monoamine oxidase by Syzygium cumini leaves extract and preliminary assessment in animal models

Syzygium cumini (Myrtaceae) is a tropical tree used in traditional medicines to treat several diseases. All parts of S. cumini are used for medicinal purposes and present antioxidant potential. In this study, we evaluated the phytochemical composition of the ethanolic extract of S. cumini leaves (EE...

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Autores: Borba, Laryssa Alves [UNESP], Wiltenburg, Victor Distefano, Negri, Giuseppina, Ibe, Mariana Bittencourt [UNESP], Santos, Lucinéia dos [UNESP], Mendes, Fúlvio Rieli
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:Brasil
Institución:Universidade Estadual Paulista (UNESP)
Repositorio:Repositório Institucional da UNESP
Idioma:inglés
OAI Identifier:oai:repositorio.unesp.br:11449/233878
Acceso en línea:http://dx.doi.org/10.1016/j.sajb.2021.11.041
http://hdl.handle.net/11449/233878
Access Level:acceso abierto
Palabra clave:Acetylcholinesterase inhibition
Flavonoids glycosides
Hydrolysable tannins
Monoamine oxidase inhibition
Syzygium cumini
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spelling In vitro inhibition of acetylcholinesterase and monoamine oxidase by Syzygium cumini leaves extract and preliminary assessment in animal modelsAcetylcholinesterase inhibitionFlavonoids glycosidesHydrolysable tanninsMonoamine oxidase inhibitionSyzygium cuminiSyzygium cumini (Myrtaceae) is a tropical tree used in traditional medicines to treat several diseases. All parts of S. cumini are used for medicinal purposes and present antioxidant potential. In this study, we evaluated the phytochemical composition of the ethanolic extract of S. cumini leaves (EESC) and its biological activity in vitro and in vivo regarding some effects on the central nervous system. Behavioral tests of anxiety, depression, and memory were performed with mice and the cerebral cortex used to evaluate lipoperoxidation and the acetylcholinesterase activity ex vivo. The EESC showed the presence of flavonoids, tannins, and gallic acid esters-4-O-glucurosides, detected for the first time in the species. The EESC presented antioxidant capacity in vitro with EC50 of 82.8 μg/mL in the DPPH assay and IC50 of 1.27 μg/mL in the lipoperoxidation test. The EESC inhibited the in vitro acetylcholinesterase and monoamine oxidase activities presenting IC50 of 44.54 and 432.7 μg/mL, respectively. However, the EESC did not show significant effects on the behavioral tests and ex vivo assays. Our results confirm the potential of EESC as a source of bioactive compounds acting on important target enzymes for central nervous system disorders, but the in vivo effects must be better evaluated.Department of Biotechnology School of Sciences Humanities and Languages São Paulo State University (UNESP), Avenida Dom Antônio, 2100Centro de Ciências Naturais e Humanas Universidade Federal do ABC, Rua Arcturus, 03Departamento de Medicina Preventiva Universidade Federal de São Paulo, Rua Botucatu, 740, 4º andarDepartment of Biotechnology School of Sciences Humanities and Languages São Paulo State University (UNESP), Avenida Dom Antônio, 2100Universidade Estadual Paulista (UNESP)Universidade Federal do ABC (UFABC)Universidade Federal de São Paulo (UNIFESP)2022-05-01T11:07:38Z2022-05-01T11:07:38Z2022-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article553-563http://dx.doi.org/10.1016/j.sajb.2021.11.041South African Journal of Botany, v. 146, p. 553-563.0254-6299http://hdl.handle.net/11449/23387810.1016/j.sajb.2021.11.0412-s2.0-85120732637Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengSouth African Journal of Botanyinfo:eu-repo/semantics/openAccessBorba, Laryssa Alves [UNESP]Wiltenburg, Victor DistefanoNegri, GiuseppinaIbe, Mariana Bittencourt [UNESP]Santos, Lucinéia dos [UNESP]Mendes, Fúlvio Rieli2025-05-28T05:04:02Zoai:repositorio.unesp.br:11449/233878Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462025-05-28T05:04:02Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv In vitro inhibition of acetylcholinesterase and monoamine oxidase by Syzygium cumini leaves extract and preliminary assessment in animal models
title In vitro inhibition of acetylcholinesterase and monoamine oxidase by Syzygium cumini leaves extract and preliminary assessment in animal models
spellingShingle In vitro inhibition of acetylcholinesterase and monoamine oxidase by Syzygium cumini leaves extract and preliminary assessment in animal models
Borba, Laryssa Alves [UNESP]
Acetylcholinesterase inhibition
Flavonoids glycosides
Hydrolysable tannins
Monoamine oxidase inhibition
Syzygium cumini
title_short In vitro inhibition of acetylcholinesterase and monoamine oxidase by Syzygium cumini leaves extract and preliminary assessment in animal models
title_full In vitro inhibition of acetylcholinesterase and monoamine oxidase by Syzygium cumini leaves extract and preliminary assessment in animal models
title_fullStr In vitro inhibition of acetylcholinesterase and monoamine oxidase by Syzygium cumini leaves extract and preliminary assessment in animal models
title_full_unstemmed In vitro inhibition of acetylcholinesterase and monoamine oxidase by Syzygium cumini leaves extract and preliminary assessment in animal models
title_sort In vitro inhibition of acetylcholinesterase and monoamine oxidase by Syzygium cumini leaves extract and preliminary assessment in animal models
dc.creator.none.fl_str_mv Borba, Laryssa Alves [UNESP]
Wiltenburg, Victor Distefano
Negri, Giuseppina
Ibe, Mariana Bittencourt [UNESP]
Santos, Lucinéia dos [UNESP]
Mendes, Fúlvio Rieli
author Borba, Laryssa Alves [UNESP]
author_facet Borba, Laryssa Alves [UNESP]
Wiltenburg, Victor Distefano
Negri, Giuseppina
Ibe, Mariana Bittencourt [UNESP]
Santos, Lucinéia dos [UNESP]
Mendes, Fúlvio Rieli
author_role author
author2 Wiltenburg, Victor Distefano
Negri, Giuseppina
Ibe, Mariana Bittencourt [UNESP]
Santos, Lucinéia dos [UNESP]
Mendes, Fúlvio Rieli
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Universidade Federal do ABC (UFABC)
Universidade Federal de São Paulo (UNIFESP)
dc.subject.por.fl_str_mv Acetylcholinesterase inhibition
Flavonoids glycosides
Hydrolysable tannins
Monoamine oxidase inhibition
Syzygium cumini
topic Acetylcholinesterase inhibition
Flavonoids glycosides
Hydrolysable tannins
Monoamine oxidase inhibition
Syzygium cumini
description Syzygium cumini (Myrtaceae) is a tropical tree used in traditional medicines to treat several diseases. All parts of S. cumini are used for medicinal purposes and present antioxidant potential. In this study, we evaluated the phytochemical composition of the ethanolic extract of S. cumini leaves (EESC) and its biological activity in vitro and in vivo regarding some effects on the central nervous system. Behavioral tests of anxiety, depression, and memory were performed with mice and the cerebral cortex used to evaluate lipoperoxidation and the acetylcholinesterase activity ex vivo. The EESC showed the presence of flavonoids, tannins, and gallic acid esters-4-O-glucurosides, detected for the first time in the species. The EESC presented antioxidant capacity in vitro with EC50 of 82.8 μg/mL in the DPPH assay and IC50 of 1.27 μg/mL in the lipoperoxidation test. The EESC inhibited the in vitro acetylcholinesterase and monoamine oxidase activities presenting IC50 of 44.54 and 432.7 μg/mL, respectively. However, the EESC did not show significant effects on the behavioral tests and ex vivo assays. Our results confirm the potential of EESC as a source of bioactive compounds acting on important target enzymes for central nervous system disorders, but the in vivo effects must be better evaluated.
publishDate 2022
dc.date.none.fl_str_mv 2022-05-01T11:07:38Z
2022-05-01T11:07:38Z
2022-05-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.sajb.2021.11.041
South African Journal of Botany, v. 146, p. 553-563.
0254-6299
http://hdl.handle.net/11449/233878
10.1016/j.sajb.2021.11.041
2-s2.0-85120732637
url http://dx.doi.org/10.1016/j.sajb.2021.11.041
http://hdl.handle.net/11449/233878
identifier_str_mv South African Journal of Botany, v. 146, p. 553-563.
0254-6299
10.1016/j.sajb.2021.11.041
2-s2.0-85120732637
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv South African Journal of Botany
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 553-563
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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score 15,301603

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