High prevalence of unusual genotypes of Toxoplasma gondii infection in pork meat samples from Erechim, Southern Brazil

Belfort, Rubens Neto [UNIFESP]; Nussenblatt, Veronique; Rizzo, Luiz Vicente; Muccioli, Cristina [UNIFESP]; Silveira, Claudio Alberto Magalhaes [UNIFESP]; Nussenblatt, Robert; Khan, Assis; Sibley, L. David; Belfort, Rubens Junior [UNIFESP]

High prevalence of unusual genotypes of Toxoplasma gondii infection in pork meat samples from Erechim, Southern Brazil

Toxoplasmosis is the most common cause of infectious uveitis in Brazil, with a higher frequency in the South of the country. We have collected samples from porcine tongue and diaphragm obtained in both large and small abattoirs and used molecular biological technique to determine the prevalence of i...

Descripción completa

Detalles Bibliográficos
Autores:	Belfort, Rubens Neto [UNIFESP], Nussenblatt, Veronique, Rizzo, Luiz Vicente, Muccioli, Cristina [UNIFESP], Silveira, Claudio Alberto Magalhaes [UNIFESP], Nussenblatt, Robert, Khan, Assis, Sibley, L. David, Belfort, Rubens Junior [UNIFESP]
Tipo de recurso:	artículo
Estado:	Versión publicada
Fecha de publicación:	2007
País:	Brasil
Institución:	Universidade Federal de São Paulo (UNIFESP)
Repositorio:	Repositório Institucional da UNIFESP
Idioma:	inglés
OAI Identifier:	oai:repositorio.unifesp.br:11600/3570
Acceso en línea:	http://dx.doi.org/10.1590/S0001-37652007000100013 http://repositorio.unifesp.br/handle/11600/3570
Access Level:	acceso abierto
Palabra clave:	ocular toxoplasmosis Toxoplasma gondii genotypes toxoplasmose ocular genótipos

id	BR_0e91a78d7e9ca0bf6f232b223654e417
oai_identifier_str	oai:repositorio.unifesp.br:11600/3570
network_acronym_str	BR
network_name_str	Brasil
repository_id_str
spelling	High prevalence of unusual genotypes of Toxoplasma gondii infection in pork meat samples from Erechim, Southern Brazilocular toxoplasmosisToxoplasma gondiigenotypestoxoplasmose ocularToxoplasma gondiigenótiposToxoplasmosis is the most common cause of infectious uveitis in Brazil, with a higher frequency in the South of the country. We have collected samples from porcine tongue and diaphragm obtained in both large and small abattoirs and used molecular biological technique to determine the prevalence of infection and RFLP analysis to type the parasites. Seventeen out of 50 (34%) samples from the diaphragm and 33 out of 50 (66%) samples from the tongue demonstrated a positive PCR reaction for T. gondii and restriction analysis of four of the positive samples revealed that all had a type I genotype at SAG2. However, when other unlinked loci were analyzed, these strains had a type III genotype at markers BTUB, SAG3, and GRA6. One of the strains (8T) had a type II allele at SAG3, indicating it has a combination of alleles normally seen in the clonal lineages. Our sampling indicates a high prevalence of infection and suggests that unusual genotypes of T. gondii are found in Brazil even among domesticated pigs.Toxoplasmose é a causa mais comum de uveíte infecciosa no Brasil, com maior freqüência no sul do país. Coletamos amostras de diafragma e língua de porcos em pequenos e grandes abatedouros e utilizamos biologia molecular para determinar a taxa de infecção e ''DNA genotyping'' para tipar os parasitas. Dezessete das 50 amostras de diafragma (34%) e 33 das 50 amostras de língua (66%) foram positivas na reação de PCR para T. gondii. A análise restritiva e o sequenciamento do DNA em quatro amostras revelaram que todas apresentam genótipo tipo I no SAG2. No entanto, quando outros loci não ligados foram analisados, estas mesmas amostras se mostraram como tipo III nos marcadores BTUB, SAG3 e GRA6. Uma das amostras (8T) mostrava-se como tipo II no SAG3, indicando um perfil misto. Estas amostras demonstraram não só uma alta taxa de infecção, mas também genótipos incomuns que não foram observados com freqüência em estudos prévios. Nosso trabalho sugere que genótipos incomuns de T. gondii podem ser encontrados no Brasil, até mesmo em porcos domesticados.Universidade Federal de São Paulo (UNIFESP) Departamento de OftalmologiaUniversity of Maryland School of MedicineUniversidade de São Paulo ICB Departamento de ImunologiaClínica SilveiraNIH National Eye InstituteWashington University School of Medicine Department of Molecular BiologyUNIFESP, Depto. de OftalmologiaSciELOAcademia Brasileira de CiênciasUniversidade Federal de São Paulo (UNIFESP)University of Maryland School of MedicineUniversidade de São Paulo (USP)Clínica SilveiraNIH National Eye InstituteWashington University School of Medicine Department of Molecular Biology2015-06-14T13:36:45Z2015-06-14T13:36:45Z2007-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion111-114application/pdfhttp://dx.doi.org/10.1590/S0001-37652007000100013Anais da Academia Brasileira de Ciências. Academia Brasileira de Ciências, v. 79, n. 1, p. 111-114, 2007.10.1590/S0001-37652007000100013S0001-37652007000100013.pdf0001-3765S0001-37652007000100013http://repositorio.unifesp.br/handle/11600/3570ark:/48912/0013000021w5cengAnais da Academia Brasileira de Ciênciasinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPBelfort, Rubens Neto [UNIFESP]Nussenblatt, VeroniqueRizzo, Luiz VicenteMuccioli, Cristina [UNIFESP]Silveira, Claudio Alberto Magalhaes [UNIFESP]Nussenblatt, RobertKhan, AssisSibley, L. DavidBelfort, Rubens Junior [UNIFESP]2024-08-05T13:49:34Zoai:repositorio.unifesp.br:11600/3570Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-05T13:49:34Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv	High prevalence of unusual genotypes of Toxoplasma gondii infection in pork meat samples from Erechim, Southern Brazil
title	High prevalence of unusual genotypes of Toxoplasma gondii infection in pork meat samples from Erechim, Southern Brazil
spellingShingle	High prevalence of unusual genotypes of Toxoplasma gondii infection in pork meat samples from Erechim, Southern Brazil Belfort, Rubens Neto [UNIFESP] ocular toxoplasmosis Toxoplasma gondii genotypes toxoplasmose ocular Toxoplasma gondii genótipos
title_short	High prevalence of unusual genotypes of Toxoplasma gondii infection in pork meat samples from Erechim, Southern Brazil
title_full	High prevalence of unusual genotypes of Toxoplasma gondii infection in pork meat samples from Erechim, Southern Brazil
title_fullStr	High prevalence of unusual genotypes of Toxoplasma gondii infection in pork meat samples from Erechim, Southern Brazil
title_full_unstemmed	High prevalence of unusual genotypes of Toxoplasma gondii infection in pork meat samples from Erechim, Southern Brazil
title_sort	High prevalence of unusual genotypes of Toxoplasma gondii infection in pork meat samples from Erechim, Southern Brazil
dc.creator.none.fl_str_mv	Belfort, Rubens Neto [UNIFESP] Nussenblatt, Veronique Rizzo, Luiz Vicente Muccioli, Cristina [UNIFESP] Silveira, Claudio Alberto Magalhaes [UNIFESP] Nussenblatt, Robert Khan, Assis Sibley, L. David Belfort, Rubens Junior [UNIFESP]
author	Belfort, Rubens Neto [UNIFESP]
author_facet	Belfort, Rubens Neto [UNIFESP] Nussenblatt, Veronique Rizzo, Luiz Vicente Muccioli, Cristina [UNIFESP] Silveira, Claudio Alberto Magalhaes [UNIFESP] Nussenblatt, Robert Khan, Assis Sibley, L. David Belfort, Rubens Junior [UNIFESP]
author_role	author
author2	Nussenblatt, Veronique Rizzo, Luiz Vicente Muccioli, Cristina [UNIFESP] Silveira, Claudio Alberto Magalhaes [UNIFESP] Nussenblatt, Robert Khan, Assis Sibley, L. David Belfort, Rubens Junior [UNIFESP]
author2_role	author author author author author author author author
dc.contributor.none.fl_str_mv	Universidade Federal de São Paulo (UNIFESP) University of Maryland School of Medicine Universidade de São Paulo (USP) Clínica Silveira NIH National Eye Institute Washington University School of Medicine Department of Molecular Biology
dc.subject.por.fl_str_mv	ocular toxoplasmosis Toxoplasma gondii genotypes toxoplasmose ocular Toxoplasma gondii genótipos
topic	ocular toxoplasmosis Toxoplasma gondii genotypes toxoplasmose ocular Toxoplasma gondii genótipos
description	Toxoplasmosis is the most common cause of infectious uveitis in Brazil, with a higher frequency in the South of the country. We have collected samples from porcine tongue and diaphragm obtained in both large and small abattoirs and used molecular biological technique to determine the prevalence of infection and RFLP analysis to type the parasites. Seventeen out of 50 (34%) samples from the diaphragm and 33 out of 50 (66%) samples from the tongue demonstrated a positive PCR reaction for T. gondii and restriction analysis of four of the positive samples revealed that all had a type I genotype at SAG2. However, when other unlinked loci were analyzed, these strains had a type III genotype at markers BTUB, SAG3, and GRA6. One of the strains (8T) had a type II allele at SAG3, indicating it has a combination of alleles normally seen in the clonal lineages. Our sampling indicates a high prevalence of infection and suggests that unusual genotypes of T. gondii are found in Brazil even among domesticated pigs.
publishDate	2007
dc.date.none.fl_str_mv	2007-03-01 2015-06-14T13:36:45Z 2015-06-14T13:36:45Z
dc.type.driver.fl_str_mv	info:eu-repo/semantics/article
dc.type.status.fl_str_mv	info:eu-repo/semantics/publishedVersion
format	article
status_str	publishedVersion
dc.identifier.uri.fl_str_mv	http://dx.doi.org/10.1590/S0001-37652007000100013 Anais da Academia Brasileira de Ciências. Academia Brasileira de Ciências, v. 79, n. 1, p. 111-114, 2007. 10.1590/S0001-37652007000100013 S0001-37652007000100013.pdf 0001-3765 S0001-37652007000100013 http://repositorio.unifesp.br/handle/11600/3570
dc.identifier.dark.fl_str_mv	ark:/48912/0013000021w5c
url	http://dx.doi.org/10.1590/S0001-37652007000100013 http://repositorio.unifesp.br/handle/11600/3570
identifier_str_mv	Anais da Academia Brasileira de Ciências. Academia Brasileira de Ciências, v. 79, n. 1, p. 111-114, 2007. 10.1590/S0001-37652007000100013 S0001-37652007000100013.pdf 0001-3765 S0001-37652007000100013 ark:/48912/0013000021w5c
dc.language.iso.fl_str_mv	eng
language	eng
dc.relation.none.fl_str_mv	Anais da Academia Brasileira de Ciências
dc.rights.driver.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	111-114 application/pdf
dc.publisher.none.fl_str_mv	Academia Brasileira de Ciências
publisher.none.fl_str_mv	Academia Brasileira de Ciências
dc.source.none.fl_str_mv	reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP
instname_str	Universidade Federal de São Paulo (UNIFESP)
instacron_str	UNIFESP
institution	UNIFESP
reponame_str	Repositório Institucional da UNIFESP
collection	Repositório Institucional da UNIFESP
repository.name.fl_str_mv	Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv	biblioteca.csp@unifesp.br
_version_	1853670523474542592
score	15,127367

High prevalence of unusual genotypes of Toxoplasma gondii infection in pork meat samples from Erechim, Southern Brazil

Similares en LA Referencia