Insect-specific viruses and arboviruses in adult male culicids from Midwestern Brazil

Viruses were identified from male anthropophilic mosquitoes from Mato Grosso (MT) State, Midwest Brazil from February 2017 to January 2018. Mosquitoes tested included Aedes (Stegomyia) aegypti (1139 males; 84 pools), Culex quinquefasciatus (9426 males; 179 pools), Culex sp. (3 males; 3 pools) and Ps...

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Bibliographic Details
Authors: Ferreira, Raquel da Silva, Cruz, Lucinéia Claudia de Toni Aquino da, Souza, Vilma Jucineide, Neves, Nilvanei Aparecido da Silva, Souza, Victor Costa de, Franco Filho, Luciano Chaves, Lemos, Poliana da Silva, Lima, Clayton Pereira Silva de, Naveca, Felipe Gomes, Atanaka, Marina, Nunes, Márcio Roberto Teixeira, Slhessarenko, Renata Dezengrini
Format: article
Status:Published version
Publication Date:2020
Country:Brasil
Institution:Instituto Evandro Chagas (IEC)
Repository:Repositório Digital do Instituto Evandro Chagas (Patuá)
Language:English
OAI Identifier:oai:patua.iec.gov.br:iec/4184
Online Access:https://patua.iec.gov.br/handle/iec/4184
Access Level:Embargoed access
Keyword:Alphavirus / isolamento & purificação
Insetos / virologia
Culicidae / virologia
Arbovirus / patogenicidade
Mato Grosso (MT)
Flavivirus / isolamento & purificação
Description
Summary:Viruses were identified from male anthropophilic mosquitoes from Mato Grosso (MT) State, Midwest Brazil from February 2017 to January 2018. Mosquitoes tested included Aedes (Stegomyia) aegypti (1139 males; 84 pools), Culex quinquefasciatus (9426 males; 179 pools), Culex sp. (3 males; 3 pools) and Psorophora albigenu (1 male; 1 pool) collected from four cities of MT. Pools were subjected to viral RNA extraction followed by RT-PCRs specific for ten flaviviruses, five alphaviruses and Simbu serogroup of orthobunyaviruses. Positive pools were passaged three times in VERO cells (alphavirus and orthobunyavirus) or C6/36 cells (flavivirus), with isolates confirmed through RT-PCR and nucleotide sequencing. We detected pools positive for Ilhéus (1 pool), dengue serotype 4 (1), Mayaro (12), equine encephalitis virus (1) yellow fever (1), Oropouche (2), Zika (4) and chikungunya (12) viruses. High throughput sequencing of arbovirus positive pools identified 35 insect-specific viruses (ISVs) from the families Circoviridae (2), Parvoviridae (2), Totiviridae (1), Flaviviridae (1), Iflaviridae (2), Mesoniviridae (4), Nodaviridae (2), Luteoviridae (1), Phasmaviridae (1) Phenuiviridae (2), Rhabdoviridae (2), Orthomyxoviridae (1), Xinmoviridae (1), and unclassified Bunyavirales (1), unclassified Picornavirales (3), unclassified Riboviria (4) and taxon Negevirus (5). From these, five novel viruses were tentatively named Mojica circovirus, Kuia iflavirus, Muxirum negevirus, Lambada picorna-like virus and Tacuru picorna-like virus. Our findings underscore the diversity and wide geographical distribution of ISVs and arboviruses infecting male culicids.

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