Sequencing and description of the complete mitochondrial genome of Limatus durhamii (Diptera: Culicidae)

The genus Limatus (Diptera: Culicidae) are wild mosquitoes belonging to the Sabethini tribe that occurs in tropical countries and is related to transmission cycles of Orthobunyavirus (Bunyaviridae), particularly in the Amazon region. Given the unavailability of information related to evolutionary bi...

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Detalhes bibliográficos
Autores: Silva, Fábio Silva da, Nascimento, Bruna Laís Sena do, Cruz, Ana Cecília Ribeiro, Silva, Sandro Patroca da, Aragão, Andressa de Oliveira, Dias, Daniel Damous, Silva, Lucas Henrique da Silva e, Reis, Lúcia Aline Moura, Rosa, José Wilson, Vieira, Durval Bertram Rodrigues, Medeiros, Daniele Barbosa de Almeida, Nunes Neto, Joaquim Pinto
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:Brasil
Recursos:Instituto Evandro Chagas (IEC)
Repositorio:Repositório Digital do Instituto Evandro Chagas (Patuá)
Idioma:inglés
OAI Identifier:oai:patua.iec.gov.br:iec/6738
Acesso em linha:https://patua.iec.gov.br/handle/iec/6738
Access Level:acceso embargado
Palavra-chave:Culicidae / crescimento & desenvolvimento
Culicidae / classificação
Limatus durhamii / classificação
Genoma Mitocondrial / genética
Sequenciamento Completo do Genoma / métodos
Descrição
Resumo:The genus Limatus (Diptera: Culicidae) are wild mosquitoes belonging to the Sabethini tribe that occurs in tropical countries and is related to transmission cycles of Orthobunyavirus (Bunyaviridae), particularly in the Amazon region. Given the unavailability of information related to evolutionary biology and molecular taxonomy aspects of this genus, we report here the first complete sequencing of the mitochondrial genome of Limatus durhamii Theobald, 1901. The NextSeq 500 platform was used for sample sequencing, and the mitochondrial sequence obtained was 14,875 bp long, comprising 37 functional subunits (13 PCGs, 22 tRNA and 02 rRNA). The phylogeny reconstructed by maximum likelihood based on the concatenation of all 13 PCGs corroborated the known taxonomic classification based most on aspects of the external morphology and few molecular studies. The data and information produced here may be useful in the future development of taxonomic and evolutionary studies for the genus, as well as the Culicidae family itself.

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