MTHFR C677T and A1298C polymorphisms as predictors of radiotherapy response in head and neck squamous cell carcinoma

The C677T and A1298C polymorphisms in methylenetetrahydrofolate reductase (MTHFR), which regulates the release of active folate in the body, may have reduced activity. Given that folate participates in important intracellular pathways, such as nucleotide synthesis and biomolecule methylation, it see...

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Authors: Anders, Q. S., Stur, E., Agostini, Lidiane Pignaton, Garcia, F. M., Reis, R. S., Santos, J. A., Mendes, S. O., Maia, L. L., Peterle, G. T., Stange, V., Carvalho, M. B., Tajara, E. H., Santos, Marcelo dos, Silva-Conforti, A. M. A., Louro, I. D.
Format: article
Status:Published version
Publication Date:2015
Country:Brasil
Institution:Universidade Federal do Rio Grande do Norte (UFRN)
Repository:Repositório Institucional da UFRN
Language:English
OAI Identifier:oai:repositorio.ufrn.br:123456789/31481
Online Access:https://repositorio.ufrn.br/handle/123456789/31481
Access Level:Open access
Keyword:MTHFR
Head and neck cancer
Radiotherapy
Description
Summary:The C677T and A1298C polymorphisms in methylenetetrahydrofolate reductase (MTHFR), which regulates the release of active folate in the body, may have reduced activity. Given that folate participates in important intracellular pathways, such as nucleotide synthesis and biomolecule methylation, it seems plausible that patients with head and neck squamous cell carcinoma (HNSCC) may respond differently to radiotherapy treatments, based on genetic polymorphisms. Therefore, this study sought to understand the role of these polymorphisms in HNSCC patient radiotherapy response. Genotypes were detected by PCR-RFLP after extraction of DNA from peripheral blood lymphocytes. Survival curves were analyzed by the KaplanMeier model, and significant differences were analyzed by the Wilcoxon test. Response to radiotherapy in patients with laryngeal SCC was significantly associated with the MTHFR C677T polymorphism (P = 0.030). Indeed, the presence of at least one T allele decreases the mortality rate up to 3-fold. Therefore, we propose that MTHFR C677T may represent a putative biomarker for radiotherapy prognosis in laryngeal SCC patients