Stability-indicating capillary zone electrophoresis assay for the analysis of linezolid in tablets

A simple and fast alternative methodology using capillary zone electrophoresis (CZE) to analyze linezolid and its cationic photodegradation products in tablets has been developed. The separation was carried out on fused silica capillary and conducted using 100 mM formic acid (pH 3.0) and by applying...

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Detalles Bibliográficos
Autores: Oliveira, Cristiani Lopes Capistrano Gonçalves de, Salgado, Hérida Regina Nunes, Moraes, Maria de Lourdes Leite
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:Brasil
Institución:Universidade de São Paulo (USP)
Repositorio:Brazilian Journal of Pharmaceutical Sciences
Idioma:inglés
OAI Identifier:oai:revistas.usp.br:article/159291
Acceso en línea:https://www.revistas.usp.br/bjps/article/view/159291
Access Level:acceso abierto
Palabra clave:Capillary-electrophoresis/assay
Linezolid/tablets/stability
Pharmaceutical-drugs
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repository_id_str
spelling Stability-indicating capillary zone electrophoresis assay for the analysis of linezolid in tabletsCapillary-electrophoresis/assayLinezolid/tablets/stabilityPharmaceutical-drugsA simple and fast alternative methodology using capillary zone electrophoresis (CZE) to analyze linezolid and its cationic photodegradation products in tablets has been developed. The separation was carried out on fused silica capillary and conducted using 100 mM formic acid (pH 3.0) and by applying 30 KV voltage. Detection was performed at UV 254 nm. The optimized method was validated in terms of linearity, limits of detection and quantification, precision (repeatability), stability studies (selectivity) and accuracy. Good linearity (8-20 mg L-1) was obtained and the limit of detection was 0.95 mg L-1. The greatest advantages of the CZE method were the rapid set-up of instrumentation and capillary equilibration, short analysis time (12 min), low running cost and low waste generation. The method showed good stability in determining linezolid submitted to degradation by light and to a climatic chamber and can be used as an alternative for evaluation in stability studies of linezolid in tablets, as well as for the analysis of the drug in raw materials and finished products.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas2018-12-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjps/article/view/15929110.1590/s2175-97902018000417585Brazilian Journal of Pharmaceutical Sciences; Vol. 54 Núm. 4 (2018); e17585Brazilian Journal of Pharmaceutical Sciences; v. 54 n. 4 (2018); e17585Brazilian Journal of Pharmaceutical Sciences; Vol. 54 No. 4 (2018); e175852175-97901984-8250reponame:Brazilian Journal of Pharmaceutical Sciencesinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/bjps/article/view/159291/154090Copyright (c) 2018 Brazilian Journal of Pharmaceutical Sciencesinfo:eu-repo/semantics/openAccessOliveira, Cristiani Lopes Capistrano Gonçalves deSalgado, Hérida Regina NunesMoraes, Maria de Lourdes Leite2019-06-24T20:15:38Zoai:revistas.usp.br:article/159291Revistahttps://www.revistas.usp.br/bjps/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpbjps@usp.br||elizabeth.igne@gmail.com2175-97901984-8250opendoar:2019-06-24T20:15:38Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Stability-indicating capillary zone electrophoresis assay for the analysis of linezolid in tablets
title Stability-indicating capillary zone electrophoresis assay for the analysis of linezolid in tablets
spellingShingle Stability-indicating capillary zone electrophoresis assay for the analysis of linezolid in tablets
Oliveira, Cristiani Lopes Capistrano Gonçalves de
Capillary-electrophoresis/assay
Linezolid/tablets/stability
Pharmaceutical-drugs
title_short Stability-indicating capillary zone electrophoresis assay for the analysis of linezolid in tablets
title_full Stability-indicating capillary zone electrophoresis assay for the analysis of linezolid in tablets
title_fullStr Stability-indicating capillary zone electrophoresis assay for the analysis of linezolid in tablets
title_full_unstemmed Stability-indicating capillary zone electrophoresis assay for the analysis of linezolid in tablets
title_sort Stability-indicating capillary zone electrophoresis assay for the analysis of linezolid in tablets
dc.creator.none.fl_str_mv Oliveira, Cristiani Lopes Capistrano Gonçalves de
Salgado, Hérida Regina Nunes
Moraes, Maria de Lourdes Leite
author Oliveira, Cristiani Lopes Capistrano Gonçalves de
author_facet Oliveira, Cristiani Lopes Capistrano Gonçalves de
Salgado, Hérida Regina Nunes
Moraes, Maria de Lourdes Leite
author_role author
author2 Salgado, Hérida Regina Nunes
Moraes, Maria de Lourdes Leite
author2_role author
author
dc.subject.por.fl_str_mv Capillary-electrophoresis/assay
Linezolid/tablets/stability
Pharmaceutical-drugs
topic Capillary-electrophoresis/assay
Linezolid/tablets/stability
Pharmaceutical-drugs
description A simple and fast alternative methodology using capillary zone electrophoresis (CZE) to analyze linezolid and its cationic photodegradation products in tablets has been developed. The separation was carried out on fused silica capillary and conducted using 100 mM formic acid (pH 3.0) and by applying 30 KV voltage. Detection was performed at UV 254 nm. The optimized method was validated in terms of linearity, limits of detection and quantification, precision (repeatability), stability studies (selectivity) and accuracy. Good linearity (8-20 mg L-1) was obtained and the limit of detection was 0.95 mg L-1. The greatest advantages of the CZE method were the rapid set-up of instrumentation and capillary equilibration, short analysis time (12 min), low running cost and low waste generation. The method showed good stability in determining linezolid submitted to degradation by light and to a climatic chamber and can be used as an alternative for evaluation in stability studies of linezolid in tablets, as well as for the analysis of the drug in raw materials and finished products.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-20
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/bjps/article/view/159291
10.1590/s2175-97902018000417585
url https://www.revistas.usp.br/bjps/article/view/159291
identifier_str_mv 10.1590/s2175-97902018000417585
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/bjps/article/view/159291/154090
dc.rights.driver.fl_str_mv Copyright (c) 2018 Brazilian Journal of Pharmaceutical Sciences
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Brazilian Journal of Pharmaceutical Sciences
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Ciências Farmacêuticas
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Ciências Farmacêuticas
dc.source.none.fl_str_mv Brazilian Journal of Pharmaceutical Sciences; Vol. 54 Núm. 4 (2018); e17585
Brazilian Journal of Pharmaceutical Sciences; v. 54 n. 4 (2018); e17585
Brazilian Journal of Pharmaceutical Sciences; Vol. 54 No. 4 (2018); e17585
2175-9790
1984-8250
reponame:Brazilian Journal of Pharmaceutical Sciences
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Brazilian Journal of Pharmaceutical Sciences
collection Brazilian Journal of Pharmaceutical Sciences
repository.name.fl_str_mv Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP)
repository.mail.fl_str_mv bjps@usp.br||elizabeth.igne@gmail.com
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