Eletroforese de proteínas e isoenzimas em sementes de Copaifera Langsdorffii Desf. (leguminosae caesalpinioideae) envelhecidas artificialmente

Electrophoresis has been used to aid the evaluation of physiological seed quality of forest species, as isozymes provide fast responses. Seeds of copaiba (Copaifera langsdorffii) were artificially aged for 0, 24, 48, 72 and 96 hours, in a germination chamber (42º C and 100% UR). The objective of thi...

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Detalhes bibliográficos
Autores: Carvalho, Dulcinéia de, Ferreira, Robério Anastácio, Oliveira, Luciana Magda de, Oliveira, Alessandro Fabiano de, Gemaque, Rinã Celeste Rodrigues
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2006
País:Brasil
Recursos:Universidade Federal de Sergipe (UFS)
Repositorio:Repositório Institucional da UFS
Idioma:portugués
OAI Identifier:oai:oai:ri.ufs.br:repo_01:riufs/11930
Acesso em linha:http://ri.ufs.br/jspui/handle/riufs/11930
Access Level:acceso abierto
Palavra-chave:Germinação
Vigor de sementes
Eletroforese
Espécie florestal
Seed germination
Artificial aging
Electrophoresis
Copaifera langsdorffii
Descrição
Resumo:Electrophoresis has been used to aid the evaluation of physiological seed quality of forest species, as isozymes provide fast responses. Seeds of copaiba (Copaifera langsdorffii) were artificially aged for 0, 24, 48, 72 and 96 hours, in a germination chamber (42º C and 100% UR). The objective of this research was to evaluate possible changes in the electrophoretic patterns of proteins and isozymes during the artificial aging. The artificial aging reduced the germination and vigor of C. langsdorffii seeds, but vigor was affected faster than germination. The electrophoretic patterns showed no difference in the content of soluble and storage proteins, but for esterase and peroxidase isozymes there was reduction in the activity with aging.