Capture erytroimmunoadsorption test for neurocysticercosis immunodiagnosis
The capture erytroimmunoadsorption (C-EIA) test was standardized for detection of Taenia solium cysticercus-IgG specific antibodies in cerebrospinal fluid (CSF) from patients with ncurocysticercosis. For the C-EIA test performance a reagent for specific antibody detection was prepared using sheep�...
| Autores: | , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 1994 |
| País: | Brasil |
| Institución: | Universidade de São Paulo (USP) |
| Repositorio: | Revista de Saúde Pública |
| Idioma: | portugués |
| OAI Identifier: | oai:revistas.usp.br:article/24019 |
| Acceso en línea: | https://www.revistas.usp.br/rsp/article/view/24019 |
| Access Level: | acceso abierto |
| Palabra clave: | Cisticercose^i1^sdiagnóst Técnicas de imunoadsorção Anticorpos anti-helmintos^i1^sles Cysticercosis^i2^sdiagno Immunosorbent techniques Antibodies helminth^i2^scerebrospinal fl |
| Sumario: | The capture erytroimmunoadsorption (C-EIA) test was standardized for detection of Taenia solium cysticercus-IgG specific antibodies in cerebrospinal fluid (CSF) from patients with ncurocysticercosis. For the C-EIA test performance a reagent for specific antibody detection was prepared using sheep's red blood cells (SRBC) in a concentration of 0.25% sensitized with crude saline extract antigen (SEA) obtained from Cysticercus cellulosae. The optimum concentration of SEA for SRBC sensitization was 40ug/ml. The yield of SEA was 0.lug protein/cavity. When 58 CSF samples from patients with neurocysticercosis were analysed the sensitivity of the test was found to be 84.5% and the confidence limit of 95% probability (CL 95%) ranged from 75% to 94%. The specificity was 95.3% (CL 95% from 90.7% to 99.9%) when CSF samples from the control group were analysed. The C-EIA test was shown to be efficient for neurocysticercosis diagnosis and important for public health laboratories, because of its low cost, high reagent yield and ease of use. |
|---|