Evaluation of the composition of culture medium for yeast biomass production using raw glycerol from biodiesel synthesis

The work herewith investigated the production of yeast biomass as a source of protein, using Yarrowia lipolytica NRRL YB-423 and raw glycerol from biodiesel synthesis as the main carbon source. A significant influence of glycerol concentration, initial pH and yeast extract concentration on biomass a...

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Detalles Bibliográficos
Autores: Santos, Elisane Odriosolla dos, Michelon, Mariano, Furlong, Eliana Badiale, Burkert, Janaina Fernandes de Medeiros, Kalil, Susana Juliano, Burkert, Carlos André Veiga
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2012
País:Brasil
Institución:Universidade Federal do Rio Grande (FURG)
Repositorio:Repositório Institucional da FURG (RI FURG)
Idioma:inglés
OAI Identifier:oai:repositorio.furg.br:1/4427
Acceso en línea:http://repositorio.furg.br/handle/1/4427
Access Level:acceso abierto
Palabra clave:Raw glycerol
single cell protein
yeast biomass
Yarrowia lipolytica
Descripción
Sumario:The work herewith investigated the production of yeast biomass as a source of protein, using Yarrowia lipolytica NRRL YB-423 and raw glycerol from biodiesel synthesis as the main carbon source. A significant influence of glycerol concentration, initial pH and yeast extract concentration on biomass and protein content was observed according to the 2v 5-1 fractional design. These factors were further evaluated using a central composite design and response surface methodology, and an empirical model for protein content was established and validated. The biomass of Yarrowia lipolytica NRRL YB-423 reached 19.5 ± 1.0 g/L in shaken flasks cultivation, with a protein content of 20.1 ± 0.6% (w/w).