Weaning stress influences the in vitro response of the tumor necrosis factor in Mangalarga Marchador foals

This study aimed to evaluate the changes caused by the abrupt weaning of plasma total cortisol and the occurrence of cell immunosuppression, measured by the expression of tumour necrosis factor alpha (TNF-α). The abrupt weaning was performed in eight Mangalarga Marchador foals aged between 24 and 28...

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Detalhes bibliográficos
Autores: Spindola, Bruno Ferreira, Botteon, Paulo de Tarso Landgraf, da Rocha, Fabio Fagundes, Calado, Simone Bizerra, Botteon, Rita de Cassia Campbell Machado, Rodrigues, Isabella Manes Souto Mayor da Mota, Marinho, Lucas Moura
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:Brasil
Recursos:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)
Repositorio:Brazilian Journal of Veterinary Medicine
Idioma:portugués
OAI Identifier:oai:ojs.rbmv.org:article/932
Acesso em linha:https://bjvm.org.br/BJVM/article/view/932
Access Level:acceso abierto
Palavra-chave:Cortisol, potro, estresse, imunidade celular, TNF-α.
Cortisol, cellular immunity, foal, stress, TNF-α.
Descrição
Resumo:This study aimed to evaluate the changes caused by the abrupt weaning of plasma total cortisol and the occurrence of cell immunosuppression, measured by the expression of tumour necrosis factor alpha (TNF-α). The abrupt weaning was performed in eight Mangalarga Marchador foals aged between 24 and 28 weeks. The animals were evaluated in four moments: seven days before weaning (DAD), and one day after weaning (DApD), seven DApD and 15 DApD.  In these moments, blood samples were taken for determination of total plasma cortisol and the expression in vitro of TNF-α treated with lipopolysaccharide from E. coli (LPS) or dexamethasone (Dx). There was an increase of total plasma cortisol 24 hours after weaning (1 DAPD) (P <0.0001), time that the expression in vitro of TNF-α showed the lowest value in the LPS group, similar to the control group and the Dx group. After 15 DAPD, in vitro response of TNF-α was significantly higher in the LPS group (P = 0.0006). It was concluded that weaning stress provokes an acute condition in foals, which resulted in a decrease of cell-mediated immunity, expressed by lower TNF-α levels