Cold atmospheric pressure plasma jet modulates Candida albicans virulence traits
The occurrence of Candida-related infections depends on the interplay between fungal virulence factors and host's immune system. For this reason, interfering with fungal virulence is a promising approach to prevent or treat such diseases. The goal of this study was to analyze the effects of col...
| Autores: | , , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2017 |
| País: | Brasil |
| Institución: | Universidade Estadual Paulista (UNESP) |
| Repositorio: | Repositório Institucional da UNESP |
| Idioma: | inglés |
| OAI Identifier: | oai:repositorio.unesp.br:11449/179376 |
| Acceso en línea: | http://dx.doi.org/10.1016/j.cpme.2017.06.002 http://hdl.handle.net/11449/179376 |
| Access Level: | acceso abierto |
| Palabra clave: | Antifungal Biofilms Candida albicans Cytotoxicity Plasma Virulence |
| Sumario: | The occurrence of Candida-related infections depends on the interplay between fungal virulence factors and host's immune system. For this reason, interfering with fungal virulence is a promising approach to prevent or treat such diseases. The goal of this study was to analyze the effects of cold atmospheric pressure plasma jet operated with Helium (He-CAPPJ) on C. albicans virulence traits and biofilms. The study also aimed to evaluate the cytotoxicity and genotoxicity of He-CAPPJ to Vero cells. The effective operational parameters of He-CAPPJ against C. albicans SC 5314 were determined. Sub-inhibitory times of exposure were used to evaluate the effect of He-CAPPJ on morphogenesis (yeast-hyphae transition), adherence to epithelial cells, and exoenzymes production. Cytotoxicity to Vero cells was also assessed by MTT assay and genotoxicity was investigated by micronucleus test. A promising effect of He-CAPPJ on morphogenesis was observed, with a reduction of almost 40 times in the filamentation rate compared to the non-exposed group. He-CAPPJ was able to reduce C. albicans adherence and to decrease fungal biofilm viability. No effect on exoenzymes production was observed. In conclusion, He-CAPPJ was able to attenuate fungal adherence and morphogenesis with low cytotoxicity after short treatment times. Periods of exposure effective against biofilms were cytotoxic to Vero cells. He-CAPPJ did not show genotoxic effect. Therefore, He-CAPPJ has potential to be applied as an adjuvant in the treatment of C. albicans superficial infections. |
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