TRIB3 is a biomarker of poor prognosis in laryngeal squamous cell carcinoma and may affect tumor development through PI3K / AKT / mTOR pathway

Objective: TRIB3 has been confirmed to participate in and regulate biological metabolic activities in head and neck tumors such as nasopharyngeal carcinoma and oropharyngeal carcinoma, so the purpose of this study was to explore whether there is a correlation between TRIB3 and Laryngeal Squamous Cel...

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Detalles Bibliográficos
Autores: Yuan, Runsheng, Cheng, Zhongqiang, Zhan, Xiaodong
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:Brasil
Institución:Universidade de São Paulo (USP)
Repositorio:Clinics
Idioma:inglés
OAI Identifier:oai:revistas.usp.br:article/238122
Acceso en línea:https://revistas.usp.br/clinics/article/view/238122
Access Level:acceso abierto
Palabra clave:TRIB3
Laryngeal Squamous Cell Carcinoma
Prognosis
Biomarkers
Immunotherapy
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repository_id_str
spelling TRIB3 is a biomarker of poor prognosis in laryngeal squamous cell carcinoma and may affect tumor development through PI3K / AKT / mTOR pathwayTRIB3Laryngeal Squamous Cell CarcinomaPrognosisBiomarkersImmunotherapyObjective: TRIB3 has been confirmed to participate in and regulate biological metabolic activities in head and neck tumors such as nasopharyngeal carcinoma and oropharyngeal carcinoma, so the purpose of this study was to explore whether there is a correlation between TRIB3 and Laryngeal Squamous Cell Carcinoma (LSCC) and to preliminarily explore the biological characteristics of TRIB3 in LSCC. Methods: TRIB3 expression in the LSCC was analyzed based on The Cancer Genome Atlas (TCGA) database. CCK8 assay, Colony Formation Assay, wound healing assay, and Transwell assay were performed to investigate the roles of TRIB3 in the proliferation, invasion and metastasis of LSCC. The Protein-Protein Interaction (PPI) network of TRIB3 was analyzed based on the STRING database. Western blotting and qRT-PCR were employed to detect the protein and mRNA expression of TRIB3. Results: TRIB3 over-expressed in the LSCC, which was related to the poor prognosis of LSCC. Patients with methylation related to high TRIB3 expression had a poorer prognosis. Knock-down of TRIB3 expression suppresses the growth, invasion and migration of LSCCs via PI3K / AKT / mTOR. TIME analysis, surface checkpoint analysis, and prediction model indicated that TRIB3 related risk model displayed a poor prognosis. Conclusion: This study investigates the role of TRIB3 in the biological characteristics of LSCC, and preliminarily concludes that TRIB3 may be a potentially promising prognostic biomarker for LSCC. TRIB3 may facilitate the growing, invasive and migrating LSCC, leading to a poor prognosis.Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2025-01-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistas.usp.br/clinics/article/view/23812210.1016/Clinics; Vol. 80 (2025); 100576Clinics; v. 80 (2025); 100576Clinics; Vol. 80 (2025); 1005761980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://revistas.usp.br/clinics/article/view/238122/214960Copyright (c) 2025 Clinicsinfo:eu-repo/semantics/openAccessYuan, RunshengCheng, ZhongqiangZhan, Xiaodong2025-09-12T14:10:46Zoai:revistas.usp.br:article/238122Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2025-09-12T14:10:46Clinics - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv TRIB3 is a biomarker of poor prognosis in laryngeal squamous cell carcinoma and may affect tumor development through PI3K / AKT / mTOR pathway
title TRIB3 is a biomarker of poor prognosis in laryngeal squamous cell carcinoma and may affect tumor development through PI3K / AKT / mTOR pathway
spellingShingle TRIB3 is a biomarker of poor prognosis in laryngeal squamous cell carcinoma and may affect tumor development through PI3K / AKT / mTOR pathway
Yuan, Runsheng
TRIB3
Laryngeal Squamous Cell Carcinoma
Prognosis
Biomarkers
Immunotherapy
title_short TRIB3 is a biomarker of poor prognosis in laryngeal squamous cell carcinoma and may affect tumor development through PI3K / AKT / mTOR pathway
title_full TRIB3 is a biomarker of poor prognosis in laryngeal squamous cell carcinoma and may affect tumor development through PI3K / AKT / mTOR pathway
title_fullStr TRIB3 is a biomarker of poor prognosis in laryngeal squamous cell carcinoma and may affect tumor development through PI3K / AKT / mTOR pathway
title_full_unstemmed TRIB3 is a biomarker of poor prognosis in laryngeal squamous cell carcinoma and may affect tumor development through PI3K / AKT / mTOR pathway
title_sort TRIB3 is a biomarker of poor prognosis in laryngeal squamous cell carcinoma and may affect tumor development through PI3K / AKT / mTOR pathway
dc.creator.none.fl_str_mv Yuan, Runsheng
Cheng, Zhongqiang
Zhan, Xiaodong
author Yuan, Runsheng
author_facet Yuan, Runsheng
Cheng, Zhongqiang
Zhan, Xiaodong
author_role author
author2 Cheng, Zhongqiang
Zhan, Xiaodong
author2_role author
author
dc.subject.por.fl_str_mv TRIB3
Laryngeal Squamous Cell Carcinoma
Prognosis
Biomarkers
Immunotherapy
topic TRIB3
Laryngeal Squamous Cell Carcinoma
Prognosis
Biomarkers
Immunotherapy
description Objective: TRIB3 has been confirmed to participate in and regulate biological metabolic activities in head and neck tumors such as nasopharyngeal carcinoma and oropharyngeal carcinoma, so the purpose of this study was to explore whether there is a correlation between TRIB3 and Laryngeal Squamous Cell Carcinoma (LSCC) and to preliminarily explore the biological characteristics of TRIB3 in LSCC. Methods: TRIB3 expression in the LSCC was analyzed based on The Cancer Genome Atlas (TCGA) database. CCK8 assay, Colony Formation Assay, wound healing assay, and Transwell assay were performed to investigate the roles of TRIB3 in the proliferation, invasion and metastasis of LSCC. The Protein-Protein Interaction (PPI) network of TRIB3 was analyzed based on the STRING database. Western blotting and qRT-PCR were employed to detect the protein and mRNA expression of TRIB3. Results: TRIB3 over-expressed in the LSCC, which was related to the poor prognosis of LSCC. Patients with methylation related to high TRIB3 expression had a poorer prognosis. Knock-down of TRIB3 expression suppresses the growth, invasion and migration of LSCCs via PI3K / AKT / mTOR. TIME analysis, surface checkpoint analysis, and prediction model indicated that TRIB3 related risk model displayed a poor prognosis. Conclusion: This study investigates the role of TRIB3 in the biological characteristics of LSCC, and preliminarily concludes that TRIB3 may be a potentially promising prognostic biomarker for LSCC. TRIB3 may facilitate the growing, invasive and migrating LSCC, leading to a poor prognosis.
publishDate 2025
dc.date.none.fl_str_mv 2025-01-27
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistas.usp.br/clinics/article/view/238122
10.1016/
url https://revistas.usp.br/clinics/article/view/238122
identifier_str_mv 10.1016/
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://revistas.usp.br/clinics/article/view/238122/214960
dc.rights.driver.fl_str_mv Copyright (c) 2025 Clinics
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2025 Clinics
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo
publisher.none.fl_str_mv Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo
dc.source.none.fl_str_mv Clinics; Vol. 80 (2025); 100576
Clinics; v. 80 (2025); 100576
Clinics; Vol. 80 (2025); 100576
1980-5322
1807-5932
reponame:Clinics
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Clinics
collection Clinics
repository.name.fl_str_mv Clinics - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||clinics@hc.fm.usp.br
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