In vivo dendritic cell depletion reduces breeding efficiency, affecting implantation and early placental development in mice

Implantation of mammalian embryos into their mother's uterus ensures optimal nourishment and protection throughout development. Complex molecular interactions characterize the implantation process, and an optimal synchronization of the components of this embryo-maternal dialogue is crucial for...

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Autores: Krey, Gesa, Frank, Pierre, Shaikly, Valerie, Barrientos, Gabriela Laura, Cordo Russo, Rosalia Ines, Ringel, Frauke, Moschansky, Petra, Chernukhin, Igor V., Metodiev, Metodi, Fernández, Nelson, Klapp, Burghard F., Arck, Petra C., Blois, Sandra M.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2008
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/25780
Acceso en línea:http://hdl.handle.net/11336/25780
Access Level:acceso abierto
Palabra clave:Dendritic Cells
Implantation
Natural Killer Cells
Placentation
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
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network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv In vivo dendritic cell depletion reduces breeding efficiency, affecting implantation and early placental development in mice
title In vivo dendritic cell depletion reduces breeding efficiency, affecting implantation and early placental development in mice
spellingShingle In vivo dendritic cell depletion reduces breeding efficiency, affecting implantation and early placental development in mice
Krey, Gesa
Dendritic Cells
Implantation
Natural Killer Cells
Placentation
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
title_short In vivo dendritic cell depletion reduces breeding efficiency, affecting implantation and early placental development in mice
title_full In vivo dendritic cell depletion reduces breeding efficiency, affecting implantation and early placental development in mice
title_fullStr In vivo dendritic cell depletion reduces breeding efficiency, affecting implantation and early placental development in mice
title_full_unstemmed In vivo dendritic cell depletion reduces breeding efficiency, affecting implantation and early placental development in mice
title_sort In vivo dendritic cell depletion reduces breeding efficiency, affecting implantation and early placental development in mice
dc.creator.none.fl_str_mv Krey, Gesa
Frank, Pierre
Shaikly, Valerie
Barrientos, Gabriela Laura
Cordo Russo, Rosalia Ines
Ringel, Frauke
Moschansky, Petra
Chernukhin, Igor V.
Metodiev, Metodi
Fernández, Nelson
Klapp, Burghard F.
Arck, Petra C.
Blois, Sandra M.
author Krey, Gesa
author_facet Krey, Gesa
Frank, Pierre
Shaikly, Valerie
Barrientos, Gabriela Laura
Cordo Russo, Rosalia Ines
Ringel, Frauke
Moschansky, Petra
Chernukhin, Igor V.
Metodiev, Metodi
Fernández, Nelson
Klapp, Burghard F.
Arck, Petra C.
Blois, Sandra M.
author_role author
author2 Frank, Pierre
Shaikly, Valerie
Barrientos, Gabriela Laura
Cordo Russo, Rosalia Ines
Ringel, Frauke
Moschansky, Petra
Chernukhin, Igor V.
Metodiev, Metodi
Fernández, Nelson
Klapp, Burghard F.
Arck, Petra C.
Blois, Sandra M.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Dendritic Cells
Implantation
Natural Killer Cells
Placentation
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
topic Dendritic Cells
Implantation
Natural Killer Cells
Placentation
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
description Implantation of mammalian embryos into their mother's uterus ensures optimal nourishment and protection throughout development. Complex molecular interactions characterize the implantation process, and an optimal synchronization of the components of this embryo-maternal dialogue is crucial for a successful reproductive outcome. In the present study, we investigated the role of dendritic cells (DC) during implantation process using a transgenic mouse system (DTRtg) that allows transient depletion of CD11c+ cells in vivo through administration of diphtheria toxin. We observed that DC depletion impairs the implantation process, resulting in a reduced breeding efficiency. Furthermore, the maturity of uterine natural killer cells at dendritic cell knockout (DCKO) implantation sites was affected as well; as demonstrated by decreased perforin expression and reduced numbers of periodic-acid-Schiff (PAS)-positive cells. This was accompanied by disarrangements in decidual vascular development. In the present study, we were also able to identify a novel DC-dependent protein, phosphatidylinositol transfer protein beta (PITPbeta), involved in implantation and trophoblast development using a proteomic approach. Indeed, DCKO mice exhibited substantial anomalies in placental development, including hypocellularity of the spongiotrophoblast and labyrinthine layers and reduced numbers of trophoblast giant cells. Giant cells also down-regulated their expression of two characteristic markers of trophoblast differentiation, placental lactogen 1 and proliferin. In view of these findings, dendritic cells emerge as possible modulators in the orchestration of events leading to the establishment and maintenance of pregnancy.
publishDate 2008
dc.date.none.fl_str_mv 2008-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/25780
Krey, Gesa; Frank, Pierre; Shaikly, Valerie; Barrientos, Gabriela Laura; Cordo Russo, Rosalia Ines; et al.; In vivo dendritic cell depletion reduces breeding efficiency, affecting implantation and early placental development in mice; Springer; Journal of Molecular Medicine (Berlin, Germany); 86; 9-2008; 999-1011
0946-2716
1432-1440
CONICET Digital
CONICET
url http://hdl.handle.net/11336/25780
identifier_str_mv Krey, Gesa; Frank, Pierre; Shaikly, Valerie; Barrientos, Gabriela Laura; Cordo Russo, Rosalia Ines; et al.; In vivo dendritic cell depletion reduces breeding efficiency, affecting implantation and early placental development in mice; Springer; Journal of Molecular Medicine (Berlin, Germany); 86; 9-2008; 999-1011
0946-2716
1432-1440
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s00109-008-0379-2
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00109-008-0379-2
info:eu-repo/semantics/altIdentifier/pmid/18575833
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799196341687025664
spelling In vivo dendritic cell depletion reduces breeding efficiency, affecting implantation and early placental development in miceKrey, GesaFrank, PierreShaikly, ValerieBarrientos, Gabriela LauraCordo Russo, Rosalia InesRingel, FraukeMoschansky, PetraChernukhin, Igor V.Metodiev, MetodiFernández, NelsonKlapp, Burghard F.Arck, Petra C.Blois, Sandra M.Dendritic CellsImplantationNatural Killer CellsPlacentationhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Implantation of mammalian embryos into their mother's uterus ensures optimal nourishment and protection throughout development. Complex molecular interactions characterize the implantation process, and an optimal synchronization of the components of this embryo-maternal dialogue is crucial for a successful reproductive outcome. In the present study, we investigated the role of dendritic cells (DC) during implantation process using a transgenic mouse system (DTRtg) that allows transient depletion of CD11c+ cells in vivo through administration of diphtheria toxin. We observed that DC depletion impairs the implantation process, resulting in a reduced breeding efficiency. Furthermore, the maturity of uterine natural killer cells at dendritic cell knockout (DCKO) implantation sites was affected as well; as demonstrated by decreased perforin expression and reduced numbers of periodic-acid-Schiff (PAS)-positive cells. This was accompanied by disarrangements in decidual vascular development. In the present study, we were also able to identify a novel DC-dependent protein, phosphatidylinositol transfer protein beta (PITPbeta), involved in implantation and trophoblast development using a proteomic approach. Indeed, DCKO mice exhibited substantial anomalies in placental development, including hypocellularity of the spongiotrophoblast and labyrinthine layers and reduced numbers of trophoblast giant cells. Giant cells also down-regulated their expression of two characteristic markers of trophoblast differentiation, placental lactogen 1 and proliferin. In view of these findings, dendritic cells emerge as possible modulators in the orchestration of events leading to the establishment and maintenance of pregnancy.Fil: Krey, Gesa. Universität zu Berlin; AlemaniaFil: Frank, Pierre. Universität zu Berlin; AlemaniaFil: Shaikly, Valerie. University Of Essex; Reino UnidoFil: Barrientos, Gabriela Laura. Universität zu Berlin; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cordo Russo, Rosalia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universität zu Berlin; AlemaniaFil: Ringel, Frauke. Universität zu Berlin; AlemaniaFil: Moschansky, Petra. Universität zu Berlin; AlemaniaFil: Chernukhin, Igor V.. University Of Essex; Reino UnidoFil: Metodiev, Metodi. University Of Essex; Reino UnidoFil: Fernández, Nelson. University Of Essex; Reino UnidoFil: Klapp, Burghard F.. Universität zu Berlin; AlemaniaFil: Arck, Petra C.. Universität zu Berlin; AlemaniaFil: Blois, Sandra M.. Universität zu Berlin; AlemaniaSpringer2008-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/25780Krey, Gesa; Frank, Pierre; Shaikly, Valerie; Barrientos, Gabriela Laura; Cordo Russo, Rosalia Ines; et al.; In vivo dendritic cell depletion reduces breeding efficiency, affecting implantation and early placental development in mice; Springer; Journal of Molecular Medicine (Berlin, Germany); 86; 9-2008; 999-10110946-27161432-1440CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s00109-008-0379-2info:eu-repo/semantics/altIdentifier/doi/10.1007/s00109-008-0379-2info:eu-repo/semantics/altIdentifier/pmid/18575833info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T14:23:53Zoai:ri.conicet.gov.ar:11336/25780instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 14:23:53.358CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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