Studies in Diatrypaceae: the new species Eutypa microasca and investigation of ligninolytic enzyme production

In this study the extracellular production of ligninolytic enzymes by xylariaceous and diatrypaceous species from Argentina was investigated. Among them, a new species (Eutypa microasca) was evaluated. Its morphological characteristics (e.g.: very small asci), along with the molecular analyses, supp...

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Detalles Bibliográficos
Autores: Grassi, Emanuel Marcelo, Pildain, Maria Belen, Levin, Laura Noemi, Carmaran, Cecilia Cristina
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2014
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/17811
Acceso en línea:http://hdl.handle.net/11336/17811
Access Level:acceso abierto
Palabra clave:Diatrypaceae
Eutypa Microasca
Ligninolytic Enzymes
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
Descripción
Sumario:In this study the extracellular production of ligninolytic enzymes by xylariaceous and diatrypaceous species from Argentina was investigated. Among them, a new species (Eutypa microasca) was evaluated. Its morphological characteristics (e.g.: very small asci), along with the molecular analyses, support the hypothesis of the new taxon. Ligninolytic enzyme production by several saprophytic and endophytic strains belonging to the genera Xylaria, Eutypella, Eutypa and Peroneutypa was assessed on solid medium supplemented with different dyes (Poly R-478, Azure B and Malachite Green) and under submerged fermentation in copper amended malt extract/glucose medium. All the strains produced laccase in liquid cultures, and seven out of nine also produced Mn-peroxidase (highest titres detected 113 U/l). Only 5 of the strains assayed could not decolorize Malachite Green. This is the first report on laccase and Mn-peroxidase production by the members of the Diatrypaceae and contributes to the knowledge on ligninolytic enzyme production in the members of the Xylariaceae, scarcely studied up to date.