Bordetella pertussis entry into respiratory epithelial cells and intracellular survival

Bordetella pertussis is the causative agent of pertussis, aka whooping cough. Although generally considered an extracellular pathogen, this bacterium has been found inside respiratory epithelial cells, which might represent a survival strategy inside the host. Relatively little is known, however, ab...

Descripción completa

Detalles Bibliográficos
Autores: Lamberti, Yanina Andrea, Gorgojo, Juan Pablo, Massillo, Cintia Lorena, Rodriguez, Maria Eugenia
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2013
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/93467
Acceso en línea:http://hdl.handle.net/11336/93467
Access Level:acceso abierto
Palabra clave:BACTERIAL PERSISTENCE
BORDETELLA PERTUSSIS
RESPIRATORY EPITHELIAL CELLS
https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
id AR_ff0a0c86e4cfc22decb78ff22b04ea60
oai_identifier_str oai:ri.conicet.gov.ar:11336/93467
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Bordetella pertussis entry into respiratory epithelial cells and intracellular survival
title Bordetella pertussis entry into respiratory epithelial cells and intracellular survival
spellingShingle Bordetella pertussis entry into respiratory epithelial cells and intracellular survival
Lamberti, Yanina Andrea
BACTERIAL PERSISTENCE
BORDETELLA PERTUSSIS
RESPIRATORY EPITHELIAL CELLS
https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
title_short Bordetella pertussis entry into respiratory epithelial cells and intracellular survival
title_full Bordetella pertussis entry into respiratory epithelial cells and intracellular survival
title_fullStr Bordetella pertussis entry into respiratory epithelial cells and intracellular survival
title_full_unstemmed Bordetella pertussis entry into respiratory epithelial cells and intracellular survival
title_sort Bordetella pertussis entry into respiratory epithelial cells and intracellular survival
dc.creator.none.fl_str_mv Lamberti, Yanina Andrea
Gorgojo, Juan Pablo
Massillo, Cintia Lorena
Rodriguez, Maria Eugenia
author Lamberti, Yanina Andrea
author_facet Lamberti, Yanina Andrea
Gorgojo, Juan Pablo
Massillo, Cintia Lorena
Rodriguez, Maria Eugenia
author_role author
author2 Gorgojo, Juan Pablo
Massillo, Cintia Lorena
Rodriguez, Maria Eugenia
author2_role author
author
author
dc.subject.none.fl_str_mv BACTERIAL PERSISTENCE
BORDETELLA PERTUSSIS
RESPIRATORY EPITHELIAL CELLS
https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
topic BACTERIAL PERSISTENCE
BORDETELLA PERTUSSIS
RESPIRATORY EPITHELIAL CELLS
https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
description Bordetella pertussis is the causative agent of pertussis, aka whooping cough. Although generally considered an extracellular pathogen, this bacterium has been found inside respiratory epithelial cells, which might represent a survival strategy inside the host. Relatively little is known, however, about the mechanism of internalization and the fate of B. pertussis inside the epithelia. We show here that B. pertussis is able to enter those cells by a mechanism dependent on microtubule assembly, lipid raft integrity, and the activation of a tyrosine-kinase-mediated signaling. Once inside the cell, a significant proportion of the intracellular bacteria evade phagolysosomal fusion and remain viable in nonacidic lysosome-associated membrane-protein-1-negative compartments. In addition, intracellular B. pertussis was found able to repopulate the extracellular environment after complete elimination of the extracellular bacteria with polymyxin B. Taken together, these data suggest that B. pertussis is able to survive within respiratory epithelial cells and by this means potentially contribute to host immune system evasion.
publishDate 2013
dc.date.none.fl_str_mv 2013-12-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/93467
Lamberti, Yanina Andrea; Gorgojo, Juan Pablo; Massillo, Cintia Lorena; Rodriguez, Maria Eugenia; Bordetella pertussis entry into respiratory epithelial cells and intracellular survival; Oxford University Press; Pathogens and Disease; 69; 3; 1-12-2013; 194-204
2049-632X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/93467
identifier_str_mv Lamberti, Yanina Andrea; Gorgojo, Juan Pablo; Massillo, Cintia Lorena; Rodriguez, Maria Eugenia; Bordetella pertussis entry into respiratory epithelial cells and intracellular survival; Oxford University Press; Pathogens and Disease; 69; 3; 1-12-2013; 194-204
2049-632X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femspd/article/69/3/194/2398943
info:eu-repo/semantics/altIdentifier/doi/10.1111/2049-632X.12072
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799196284577382400
spelling Bordetella pertussis entry into respiratory epithelial cells and intracellular survivalLamberti, Yanina AndreaGorgojo, Juan PabloMassillo, Cintia LorenaRodriguez, Maria EugeniaBACTERIAL PERSISTENCEBORDETELLA PERTUSSISRESPIRATORY EPITHELIAL CELLShttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Bordetella pertussis is the causative agent of pertussis, aka whooping cough. Although generally considered an extracellular pathogen, this bacterium has been found inside respiratory epithelial cells, which might represent a survival strategy inside the host. Relatively little is known, however, about the mechanism of internalization and the fate of B. pertussis inside the epithelia. We show here that B. pertussis is able to enter those cells by a mechanism dependent on microtubule assembly, lipid raft integrity, and the activation of a tyrosine-kinase-mediated signaling. Once inside the cell, a significant proportion of the intracellular bacteria evade phagolysosomal fusion and remain viable in nonacidic lysosome-associated membrane-protein-1-negative compartments. In addition, intracellular B. pertussis was found able to repopulate the extracellular environment after complete elimination of the extracellular bacteria with polymyxin B. Taken together, these data suggest that B. pertussis is able to survive within respiratory epithelial cells and by this means potentially contribute to host immune system evasion.Fil: Lamberti, Yanina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Gorgojo, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Massillo, Cintia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Rodriguez, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaOxford University Press2013-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/93467Lamberti, Yanina Andrea; Gorgojo, Juan Pablo; Massillo, Cintia Lorena; Rodriguez, Maria Eugenia; Bordetella pertussis entry into respiratory epithelial cells and intracellular survival; Oxford University Press; Pathogens and Disease; 69; 3; 1-12-2013; 194-2042049-632XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femspd/article/69/3/194/2398943info:eu-repo/semantics/altIdentifier/doi/10.1111/2049-632X.12072info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T14:21:38Zoai:ri.conicet.gov.ar:11336/93467instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 14:21:39.215CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
score 15,811543

Similares en LA Referencia