Characterization of a novel GH10 alkali-thermostable xylanase from a termite microbiome

The aim of the present study was to assess the biochemical and molecular structural characteristics of a novel alkali-thermostable GH10 xylanase (Xyl10B) identified in a termite gut microbiome by a shotgun metagenomic approach. This endoxylanase candidate was amplified, cloned, heterologously expres...

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Authors: Mon, Maria Laura, Marrero Díaz de Villegas, Rubén, Campos, Eleonora, Soria, Marcelo A., Talia, Paola Monica
Format: article
Status:Published version
Publication Date:2022
Country:Argentina
Institution:Consejo Nacional de Investigaciones Científicas y Técnicas
Repository:CONICET Digital (CONICET)
Language:English
OAI Identifier:oai:ri.conicet.gov.ar:11336/213291
Online Access:http://hdl.handle.net/11336/213291
Access Level:Open access
Keyword:BIOCHEMICAL CHARACTERIZATION
ENDOXYLANASE
GH10
TERMITE GUT MICROBIOME
https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
id AR_fec4071bbe5d18e523dc80dfcf65d314
oai_identifier_str oai:ri.conicet.gov.ar:11336/213291
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Characterization of a novel GH10 alkali-thermostable xylanase from a termite microbiome
title Characterization of a novel GH10 alkali-thermostable xylanase from a termite microbiome
spellingShingle Characterization of a novel GH10 alkali-thermostable xylanase from a termite microbiome
Mon, Maria Laura
BIOCHEMICAL CHARACTERIZATION
ENDOXYLANASE
GH10
TERMITE GUT MICROBIOME
https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
title_short Characterization of a novel GH10 alkali-thermostable xylanase from a termite microbiome
title_full Characterization of a novel GH10 alkali-thermostable xylanase from a termite microbiome
title_fullStr Characterization of a novel GH10 alkali-thermostable xylanase from a termite microbiome
title_full_unstemmed Characterization of a novel GH10 alkali-thermostable xylanase from a termite microbiome
title_sort Characterization of a novel GH10 alkali-thermostable xylanase from a termite microbiome
dc.creator.none.fl_str_mv Mon, Maria Laura
Marrero Díaz de Villegas, Rubén
Campos, Eleonora
Soria, Marcelo A.
Talia, Paola Monica
author Mon, Maria Laura
author_facet Mon, Maria Laura
Marrero Díaz de Villegas, Rubén
Campos, Eleonora
Soria, Marcelo A.
Talia, Paola Monica
author_role author
author2 Marrero Díaz de Villegas, Rubén
Campos, Eleonora
Soria, Marcelo A.
Talia, Paola Monica
author2_role author
author
author
author
dc.subject.none.fl_str_mv BIOCHEMICAL CHARACTERIZATION
ENDOXYLANASE
GH10
TERMITE GUT MICROBIOME
https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
topic BIOCHEMICAL CHARACTERIZATION
ENDOXYLANASE
GH10
TERMITE GUT MICROBIOME
https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
description The aim of the present study was to assess the biochemical and molecular structural characteristics of a novel alkali-thermostable GH10 xylanase (Xyl10B) identified in a termite gut microbiome by a shotgun metagenomic approach. This endoxylanase candidate was amplified, cloned, heterologously expressed in Escherichia coli and purified. The recombinant enzyme was active at a broad range of temperatures (37–60 ºC) and pH values (4–10), with optimal activity at 50 ºC and pH 9. Moreover, its activity remained at more than 80% of its maximum at 50 °C for 8 h. In addition, Xyl10B was found to be stable in the presence of salt and several ions and chemical reagents frequently used in the industry. These characteristics make this enzyme an interesting candidate for pulp and paper bleaching industries, since this process requires enzymes without cellulase activity and resistant to high temperatures and alkaline pH (thermo-alkaliphilic enzymes). The products of xylan hydrolysis by Xyl10B (short xylooligosaccharides, xylose and xylobiose) could be suitable for application as prebiotics and in the production of bioethanol.
publishDate 2022
dc.date.none.fl_str_mv 2022-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/213291
Mon, Maria Laura; Marrero Díaz de Villegas, Rubén; Campos, Eleonora; Soria, Marcelo A.; Talia, Paola Monica; Characterization of a novel GH10 alkali-thermostable xylanase from a termite microbiome; Springer; Bioresources and Bioprocessing; 9; 1; 12-2022; 1-15
2197-4365
CONICET Digital
CONICET
url http://hdl.handle.net/11336/213291
identifier_str_mv Mon, Maria Laura; Marrero Díaz de Villegas, Rubén; Campos, Eleonora; Soria, Marcelo A.; Talia, Paola Monica; Characterization of a novel GH10 alkali-thermostable xylanase from a termite microbiome; Springer; Bioresources and Bioprocessing; 9; 1; 12-2022; 1-15
2197-4365
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://bioresourcesbioprocessing.springeropen.com/articles/10.1186/s40643-022-00572-w
info:eu-repo/semantics/altIdentifier/doi/10.1186/s40643-022-00572-w
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799194823911014400
spelling Characterization of a novel GH10 alkali-thermostable xylanase from a termite microbiomeMon, Maria LauraMarrero Díaz de Villegas, RubénCampos, EleonoraSoria, Marcelo A.Talia, Paola MonicaBIOCHEMICAL CHARACTERIZATIONENDOXYLANASEGH10TERMITE GUT MICROBIOMEhttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2The aim of the present study was to assess the biochemical and molecular structural characteristics of a novel alkali-thermostable GH10 xylanase (Xyl10B) identified in a termite gut microbiome by a shotgun metagenomic approach. This endoxylanase candidate was amplified, cloned, heterologously expressed in Escherichia coli and purified. The recombinant enzyme was active at a broad range of temperatures (37–60 ºC) and pH values (4–10), with optimal activity at 50 ºC and pH 9. Moreover, its activity remained at more than 80% of its maximum at 50 °C for 8 h. In addition, Xyl10B was found to be stable in the presence of salt and several ions and chemical reagents frequently used in the industry. These characteristics make this enzyme an interesting candidate for pulp and paper bleaching industries, since this process requires enzymes without cellulase activity and resistant to high temperatures and alkaline pH (thermo-alkaliphilic enzymes). The products of xylan hydrolysis by Xyl10B (short xylooligosaccharides, xylose and xylobiose) could be suitable for application as prebiotics and in the production of bioethanol.Fil: Mon, Maria Laura. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Marrero Díaz de Villegas, Rubén. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Campos, Eleonora. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Soria, Marcelo A.. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Microbiología Agrícola; ArgentinaFil: Talia, Paola Monica. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaSpringer2022-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/213291Mon, Maria Laura; Marrero Díaz de Villegas, Rubén; Campos, Eleonora; Soria, Marcelo A.; Talia, Paola Monica; Characterization of a novel GH10 alkali-thermostable xylanase from a termite microbiome; Springer; Bioresources and Bioprocessing; 9; 1; 12-2022; 1-152197-4365CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://bioresourcesbioprocessing.springeropen.com/articles/10.1186/s40643-022-00572-winfo:eu-repo/semantics/altIdentifier/doi/10.1186/s40643-022-00572-winfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:37:13Zoai:ri.conicet.gov.ar:11336/213291instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:37:14.049CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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