Domoic acid oxidative effects on the microalgae Phaeodactylum tricornutum

Domoic acid (DA) is a natural occurring marine biotoxin. Oxidative stress generation due to DA exposure was reported in animals, but little is known on the phytoplankton community. The aim of this work was to verify whether exposure to DA in the marine diatom Phaeodactylum tricornutum favors reactiv...

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Detalles Bibliográficos
Autores: Cabrera, Joaquin, Puntarulo, Susana Ángela, González, Paula Mariela
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/227608
Acceso en línea:http://hdl.handle.net/11336/227608
Access Level:acceso abierto
Palabra clave:ALLELOPATHY
ANTIOXIDANTS
BIOTOXIN
DIATOMS
HARMFUL ALGAE BLOOMS
OXIDATIVE STRESS
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
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spelling Domoic acid oxidative effects on the microalgae Phaeodactylum tricornutumCabrera, JoaquinPuntarulo, Susana ÁngelaGonzález, Paula MarielaALLELOPATHYANTIOXIDANTSBIOTOXINDIATOMSHARMFUL ALGAE BLOOMSOXIDATIVE STRESShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Domoic acid (DA) is a natural occurring marine biotoxin. Oxidative stress generation due to DA exposure was reported in animals, but little is known on the phytoplankton community. The aim of this work was to verify whether exposure to DA in the marine diatom Phaeodactylum tricornutum favors reactive oxygen species (ROS) generation in the intracellular environment modifying its antioxidant capacity. Active species production, non-enzymatic antioxidant content, and antioxidant enzyme activities over the three growth phases of P. tricornutum exposed to 64 µM DA were evaluated. Results obtained in exponential growing cells showed a time-depending seven-fold increase in the 2′,7′ dichlorofluorescein diacetate dye oxidation rate. Superoxide dismutase and catalase activities showed a two-fold increase, and glutathione related enzymes activities were also significantly increased in treated diatoms as compared to controls. However, glutathione and ascorbate contents significantly decreased after incubation of the cells with DA. Similar effects were observed in latent and stationary phases of cell development. These results showed that DA could cause a severe oxidant-dependent impact on a non-toxic algae.Fil: Cabrera, Joaquin. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analitica y Fisicoquímica. Cátedra de Fisicoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; ArgentinaFil: Puntarulo, Susana Ángela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analitica y Fisicoquímica. Cátedra de Fisicoquímica; ArgentinaFil: González, Paula Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analitica y Fisicoquímica. Cátedra de Fisicoquímica; ArgentinaMDPI2023-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/227608Cabrera, Joaquin; Puntarulo, Susana Ángela; González, Paula Mariela; Domoic acid oxidative effects on the microalgae Phaeodactylum tricornutum; MDPI; Life; 13; 3; 3-2023; 1-162075-1729CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2075-1729/13/3/676info:eu-repo/semantics/altIdentifier/doi/10.3390/life13030676info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:53:14Zoai:ri.conicet.gov.ar:11336/227608instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:53:14.803CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Domoic acid oxidative effects on the microalgae Phaeodactylum tricornutum
title Domoic acid oxidative effects on the microalgae Phaeodactylum tricornutum
spellingShingle Domoic acid oxidative effects on the microalgae Phaeodactylum tricornutum
Cabrera, Joaquin
ALLELOPATHY
ANTIOXIDANTS
BIOTOXIN
DIATOMS
HARMFUL ALGAE BLOOMS
OXIDATIVE STRESS
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short Domoic acid oxidative effects on the microalgae Phaeodactylum tricornutum
title_full Domoic acid oxidative effects on the microalgae Phaeodactylum tricornutum
title_fullStr Domoic acid oxidative effects on the microalgae Phaeodactylum tricornutum
title_full_unstemmed Domoic acid oxidative effects on the microalgae Phaeodactylum tricornutum
title_sort Domoic acid oxidative effects on the microalgae Phaeodactylum tricornutum
dc.creator.none.fl_str_mv Cabrera, Joaquin
Puntarulo, Susana Ángela
González, Paula Mariela
author Cabrera, Joaquin
author_facet Cabrera, Joaquin
Puntarulo, Susana Ángela
González, Paula Mariela
author_role author
author2 Puntarulo, Susana Ángela
González, Paula Mariela
author2_role author
author
dc.subject.none.fl_str_mv ALLELOPATHY
ANTIOXIDANTS
BIOTOXIN
DIATOMS
HARMFUL ALGAE BLOOMS
OXIDATIVE STRESS
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic ALLELOPATHY
ANTIOXIDANTS
BIOTOXIN
DIATOMS
HARMFUL ALGAE BLOOMS
OXIDATIVE STRESS
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description Domoic acid (DA) is a natural occurring marine biotoxin. Oxidative stress generation due to DA exposure was reported in animals, but little is known on the phytoplankton community. The aim of this work was to verify whether exposure to DA in the marine diatom Phaeodactylum tricornutum favors reactive oxygen species (ROS) generation in the intracellular environment modifying its antioxidant capacity. Active species production, non-enzymatic antioxidant content, and antioxidant enzyme activities over the three growth phases of P. tricornutum exposed to 64 µM DA were evaluated. Results obtained in exponential growing cells showed a time-depending seven-fold increase in the 2′,7′ dichlorofluorescein diacetate dye oxidation rate. Superoxide dismutase and catalase activities showed a two-fold increase, and glutathione related enzymes activities were also significantly increased in treated diatoms as compared to controls. However, glutathione and ascorbate contents significantly decreased after incubation of the cells with DA. Similar effects were observed in latent and stationary phases of cell development. These results showed that DA could cause a severe oxidant-dependent impact on a non-toxic algae.
publishDate 2023
dc.date.none.fl_str_mv 2023-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/227608
Cabrera, Joaquin; Puntarulo, Susana Ángela; González, Paula Mariela; Domoic acid oxidative effects on the microalgae Phaeodactylum tricornutum; MDPI; Life; 13; 3; 3-2023; 1-16
2075-1729
CONICET Digital
CONICET
url http://hdl.handle.net/11336/227608
identifier_str_mv Cabrera, Joaquin; Puntarulo, Susana Ángela; González, Paula Mariela; Domoic acid oxidative effects on the microalgae Phaeodactylum tricornutum; MDPI; Life; 13; 3; 3-2023; 1-16
2075-1729
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2075-1729/13/3/676
info:eu-repo/semantics/altIdentifier/doi/10.3390/life13030676
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 15,811543

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