Synergistic effect of surfactin from Bacillus subtilis C4 and Achyrocline satureioides extracts on the viability of Paenibacillus larvae

The aim of this work was to determine the in vitro effect of the mixture between the lipopeptide surfactin, synthesized by Bacillus subtilis C4 (strain isolated from honey) and the most active vegetal extract from Achyrocline satureioides, a traditional medicinal plant, on local strains of Paenibaci...

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Detalhes bibliográficos
Autores: Sabate, Daniela Constanza, Gonzalez, Maria Juliana, Porrini, Martín Pablo, Eguaras, Martin Javier, Audisio, Marcela Carina, Marioli, Juan Miguel
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2012
País:Argentina
Recursos:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositório:CONICET Digital (CONICET)
Idioma:inglês
OAI Identifier:oai:ri.conicet.gov.ar:11336/14235
Acesso em linha:http://hdl.handle.net/11336/14235
Access Level:Acceso aberto
Palavra-chave:Surfactin
Paenibacillus Larvae
Synergism
Achyrocline Satureioides
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
id AR_fd3fc0eb09f4c38aecec106ca1a970b2
oai_identifier_str oai:ri.conicet.gov.ar:11336/14235
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Synergistic effect of surfactin from Bacillus subtilis C4 and Achyrocline satureioides extracts on the viability of Paenibacillus larvae
title Synergistic effect of surfactin from Bacillus subtilis C4 and Achyrocline satureioides extracts on the viability of Paenibacillus larvae
spellingShingle Synergistic effect of surfactin from Bacillus subtilis C4 and Achyrocline satureioides extracts on the viability of Paenibacillus larvae
Sabate, Daniela Constanza
Surfactin
Paenibacillus Larvae
Synergism
Achyrocline Satureioides
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short Synergistic effect of surfactin from Bacillus subtilis C4 and Achyrocline satureioides extracts on the viability of Paenibacillus larvae
title_full Synergistic effect of surfactin from Bacillus subtilis C4 and Achyrocline satureioides extracts on the viability of Paenibacillus larvae
title_fullStr Synergistic effect of surfactin from Bacillus subtilis C4 and Achyrocline satureioides extracts on the viability of Paenibacillus larvae
title_full_unstemmed Synergistic effect of surfactin from Bacillus subtilis C4 and Achyrocline satureioides extracts on the viability of Paenibacillus larvae
title_sort Synergistic effect of surfactin from Bacillus subtilis C4 and Achyrocline satureioides extracts on the viability of Paenibacillus larvae
dc.creator.none.fl_str_mv Sabate, Daniela Constanza
Gonzalez, Maria Juliana
Porrini, Martín Pablo
Eguaras, Martin Javier
Audisio, Marcela Carina
Marioli, Juan Miguel
author Sabate, Daniela Constanza
author_facet Sabate, Daniela Constanza
Gonzalez, Maria Juliana
Porrini, Martín Pablo
Eguaras, Martin Javier
Audisio, Marcela Carina
Marioli, Juan Miguel
author_role author
author2 Gonzalez, Maria Juliana
Porrini, Martín Pablo
Eguaras, Martin Javier
Audisio, Marcela Carina
Marioli, Juan Miguel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Surfactin
Paenibacillus Larvae
Synergism
Achyrocline Satureioides
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic Surfactin
Paenibacillus Larvae
Synergism
Achyrocline Satureioides
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description The aim of this work was to determine the in vitro effect of the mixture between the lipopeptide surfactin, synthesized by Bacillus subtilis C4 (strain isolated from honey) and the most active vegetal extract from Achyrocline satureioides, a traditional medicinal plant, on local strains of Paenibacillus larvae, the agent of American Foulbrood in honeybees. Five P. larvae strains isolated in Córdoba, Argentina, were phenotypically characterized. These and 12 other P. larvae strains from different regions of Argentina were analysed. The antimicrobial activities of the essential oil, hexane (HE) and benzene extracts from A. satureioides were assessed against P. larvae and the HE showed the highest anti-P. larvae activity. A combination of the biosurfactant surfactin, produced by B. subtilis C4, and the HE of A. satureioides revealed a synergistic action on P. larvae. The effective surfactin concentration in the mixture decreased from 32 to 1 μg ml−1 and the HE concentration from 32 to 4 μg ml−1, values similar or equal to minimal inhibitory concentrations observed for oxytetracycline. The fractional inhibitory concentration index confirmed synergism in 4 strains and partial synergism in one strain. The combination of surfactin synthesized by B. subtilis C4 and the HE from A. satureioides could be a natural alternative to help beekeepers to combat the American foulbrood agent P. larvae.
publishDate 2012
dc.date.none.fl_str_mv 2012-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/14235
Sabate, Daniela Constanza; Gonzalez, Maria Juliana; Porrini, Martín Pablo; Eguaras, Martin Javier; Audisio, Marcela Carina; et al.; Synergistic effect of surfactin from Bacillus subtilis C4 and Achyrocline satureioides extracts on the viability of Paenibacillus larvae; Springer; World Journal of Microbiology; 28; 4; 4-2012; 1415-1422
1573-0972
0959-3993
url http://hdl.handle.net/11336/14235
identifier_str_mv Sabate, Daniela Constanza; Gonzalez, Maria Juliana; Porrini, Martín Pablo; Eguaras, Martin Javier; Audisio, Marcela Carina; et al.; Synergistic effect of surfactin from Bacillus subtilis C4 and Achyrocline satureioides extracts on the viability of Paenibacillus larvae; Springer; World Journal of Microbiology; 28; 4; 4-2012; 1415-1422
1573-0972
0959-3993
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s11274-011-0941-x
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs11274-011-0941-x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799194920172388352
spelling Synergistic effect of surfactin from Bacillus subtilis C4 and Achyrocline satureioides extracts on the viability of Paenibacillus larvaeSabate, Daniela ConstanzaGonzalez, Maria JulianaPorrini, Martín PabloEguaras, Martin JavierAudisio, Marcela CarinaMarioli, Juan MiguelSurfactinPaenibacillus LarvaeSynergismAchyrocline Satureioideshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The aim of this work was to determine the in vitro effect of the mixture between the lipopeptide surfactin, synthesized by Bacillus subtilis C4 (strain isolated from honey) and the most active vegetal extract from Achyrocline satureioides, a traditional medicinal plant, on local strains of Paenibacillus larvae, the agent of American Foulbrood in honeybees. Five P. larvae strains isolated in Córdoba, Argentina, were phenotypically characterized. These and 12 other P. larvae strains from different regions of Argentina were analysed. The antimicrobial activities of the essential oil, hexane (HE) and benzene extracts from A. satureioides were assessed against P. larvae and the HE showed the highest anti-P. larvae activity. A combination of the biosurfactant surfactin, produced by B. subtilis C4, and the HE of A. satureioides revealed a synergistic action on P. larvae. The effective surfactin concentration in the mixture decreased from 32 to 1 μg ml−1 and the HE concentration from 32 to 4 μg ml−1, values similar or equal to minimal inhibitory concentrations observed for oxytetracycline. The fractional inhibitory concentration index confirmed synergism in 4 strains and partial synergism in one strain. The combination of surfactin synthesized by B. subtilis C4 and the HE from A. satureioides could be a natural alternative to help beekeepers to combat the American foulbrood agent P. larvae.Fil: Sabate, Daniela Constanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación para la Industria Química (i); Argentina. Universidad Nacional de Salta; ArgentinaFil: Gonzalez, Maria Juliana. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Porrini, Martín Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; Argentina. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Biodiversidad y Biologia Experimental. Laboratorio de Artropodos; ArgentinaFil: Eguaras, Martin Javier. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; Argentina. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Biodiversidad y Biologia Experimental. Laboratorio de Artropodos; ArgentinaFil: Audisio, Marcela Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación para la Industria Química (i); Argentina. Universidad Nacional de Salta; ArgentinaFil: Marioli, Juan Miguel. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaSpringer2012-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/14235Sabate, Daniela Constanza; Gonzalez, Maria Juliana; Porrini, Martín Pablo; Eguaras, Martin Javier; Audisio, Marcela Carina; et al.; Synergistic effect of surfactin from Bacillus subtilis C4 and Achyrocline satureioides extracts on the viability of Paenibacillus larvae; Springer; World Journal of Microbiology; 28; 4; 4-2012; 1415-14221573-09720959-3993enginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s11274-011-0941-xinfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs11274-011-0941-xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:39:09Zoai:ri.conicet.gov.ar:11336/14235instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:39:09.476CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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