Multiphoton single-molecule localization by sequential excitation with light minima

Using sequential excitation with a minimum of light to localize single fluorescent molecules represented a breakthrough because it delivers 1–2 nm precision with moderate photon counts, enabling tracking and super-resolution imaging with true molecular resolution. Expanding this concept to multi-pho...

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Bibliographic Details
Authors: Masullo, Luciano Andrés, Stefani, Fernando Daniel
Format: article
Status:Published version
Publication Date:2022
Country:Argentina
Institution:Consejo Nacional de Investigaciones Científicas y Técnicas
Repository:CONICET Digital (CONICET)
Language:English
OAI Identifier:oai:ri.conicet.gov.ar:11336/203799
Online Access:http://hdl.handle.net/11336/203799
Access Level:Open access
Keyword:Single molecule
super-resolution
https://purl.org/becyt/ford/1.3
https://purl.org/becyt/ford/1
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spelling Multiphoton single-molecule localization by sequential excitation with light minimaMasullo, Luciano AndrésStefani, Fernando DanielSingle moleculesuper-resolutionhttps://purl.org/becyt/ford/1.3https://purl.org/becyt/ford/1Using sequential excitation with a minimum of light to localize single fluorescent molecules represented a breakthrough because it delivers 1–2 nm precision with moderate photon counts, enabling tracking and super-resolution imaging with true molecular resolution. Expanding this concept to multi-photon regimes may be a useful complement to reach even higher localization precision and get deeper into biological specimens.Fil: Masullo, Luciano Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Bionanociencias "Elizabeth Jares Erijman"; ArgentinaFil: Stefani, Fernando Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Bionanociencias "Elizabeth Jares Erijman"; ArgentinaSpringer2022-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/203799Masullo, Luciano Andrés; Stefani, Fernando Daniel; Multiphoton single-molecule localization by sequential excitation with light minima; Springer; Light: Science and Applications; 11; 1; 12-2022; 1-42047-7538CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41377-022-00763-2info:eu-repo/semantics/altIdentifier/doi/10.1038/s41377-022-00763-2info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T14:25:27Zoai:ri.conicet.gov.ar:11336/203799instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 14:25:28.093CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Multiphoton single-molecule localization by sequential excitation with light minima
title Multiphoton single-molecule localization by sequential excitation with light minima
spellingShingle Multiphoton single-molecule localization by sequential excitation with light minima
Masullo, Luciano Andrés
Single molecule
super-resolution
https://purl.org/becyt/ford/1.3
https://purl.org/becyt/ford/1
title_short Multiphoton single-molecule localization by sequential excitation with light minima
title_full Multiphoton single-molecule localization by sequential excitation with light minima
title_fullStr Multiphoton single-molecule localization by sequential excitation with light minima
title_full_unstemmed Multiphoton single-molecule localization by sequential excitation with light minima
title_sort Multiphoton single-molecule localization by sequential excitation with light minima
dc.creator.none.fl_str_mv Masullo, Luciano Andrés
Stefani, Fernando Daniel
author Masullo, Luciano Andrés
author_facet Masullo, Luciano Andrés
Stefani, Fernando Daniel
author_role author
author2 Stefani, Fernando Daniel
author2_role author
dc.subject.none.fl_str_mv Single molecule
super-resolution
https://purl.org/becyt/ford/1.3
https://purl.org/becyt/ford/1
topic Single molecule
super-resolution
https://purl.org/becyt/ford/1.3
https://purl.org/becyt/ford/1
description Using sequential excitation with a minimum of light to localize single fluorescent molecules represented a breakthrough because it delivers 1–2 nm precision with moderate photon counts, enabling tracking and super-resolution imaging with true molecular resolution. Expanding this concept to multi-photon regimes may be a useful complement to reach even higher localization precision and get deeper into biological specimens.
publishDate 2022
dc.date.none.fl_str_mv 2022-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/203799
Masullo, Luciano Andrés; Stefani, Fernando Daniel; Multiphoton single-molecule localization by sequential excitation with light minima; Springer; Light: Science and Applications; 11; 1; 12-2022; 1-4
2047-7538
CONICET Digital
CONICET
url http://hdl.handle.net/11336/203799
identifier_str_mv Masullo, Luciano Andrés; Stefani, Fernando Daniel; Multiphoton single-molecule localization by sequential excitation with light minima; Springer; Light: Science and Applications; 11; 1; 12-2022; 1-4
2047-7538
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41377-022-00763-2
info:eu-repo/semantics/altIdentifier/doi/10.1038/s41377-022-00763-2
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 15,812429

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