Chromophore heterogeneity and photoconversion in phytochrome crystals and solution studied by resonance Raman spectroscopy

(Graph Presented) Crystals of the photoreceptor phytochrome were studied nondestructively by resonance Raman spectroscopy. The spectra of the chromophore-binding domains (CBD) of phytochrome from Deinococcus radiodurans, from which three-dimensional structures have been determined, demonstrate the s...

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Detalhes bibliográficos
Autores: Von Stetten, David, Günther, Mina, Scheerer, Patrick, Murgida, Daniel Horacio, Mroginski, Maria Andrea, Krauß, Norbert, Lamparter, Tilman, Zhang, Junrui, Anstrom, David M., Vierstra, Richard D., Forest, Katrina T., Hildebrandt, Peter
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2008
País:Argentina
Recursos:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/83698
Acesso em linha:http://hdl.handle.net/11336/83698
Access Level:acceso abierto
Palavra-chave:Photoreceptors
Phytochrome
Protein Crystals
Raman Spectroscopy
Tetrapyrroles
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
Descrição
Resumo:(Graph Presented) Crystals of the photoreceptor phytochrome were studied nondestructively by resonance Raman spectroscopy. The spectra of the chromophore-binding domains (CBD) of phytochrome from Deinococcus radiodurans, from which three-dimensional structures have been determined, demonstrate the same chromophore structure in the parent Pr state as in solution. Unlike CBD, crystals of phytochrome from Agrobacterium tumefaciens including the CBD and the PHY domain can be photoconverted to a Meta-Rc-like state.

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