Histological, genotoxic, and biochemical effects on Cnesterodon decemmaculatus (Jenyns 1842) (Cyprinodontiformes, Poeciliidade): early response bioassays to assess the impact of receiving waters

In the present study, the toxicity of receiving waters from a highly polluted urban watercourse, the Reconquista River, Argentina, collected at a dam in the upstream part of the river was evaluated. Cnesterodon decemmaculatus, a widely distributed fish species in Pampasic rivers proposed for use in...

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Autores: Ossana, Natalia Alejandra, Baudou, Federico Gastón, Castañé, Patricia M., Tripoli, Luis, Soloneski, Sonia Maria Elsa, Ferrari, Lucrecia
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/124023
Acceso en línea:http://hdl.handle.net/11336/124023
Access Level:acceso abierto
Palabra clave:Cnesterodon decemmaculatus
receiving water assessment
comet assay
gills and liver alteration
oxidative stress
https://purl.org/becyt/ford/1.5
https://purl.org/becyt/ford/1
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network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Histological, genotoxic, and biochemical effects on Cnesterodon decemmaculatus (Jenyns 1842) (Cyprinodontiformes, Poeciliidade): early response bioassays to assess the impact of receiving waters
title Histological, genotoxic, and biochemical effects on Cnesterodon decemmaculatus (Jenyns 1842) (Cyprinodontiformes, Poeciliidade): early response bioassays to assess the impact of receiving waters
spellingShingle Histological, genotoxic, and biochemical effects on Cnesterodon decemmaculatus (Jenyns 1842) (Cyprinodontiformes, Poeciliidade): early response bioassays to assess the impact of receiving waters
Ossana, Natalia Alejandra
Cnesterodon decemmaculatus
receiving water assessment
comet assay
gills and liver alteration
oxidative stress
https://purl.org/becyt/ford/1.5
https://purl.org/becyt/ford/1
title_short Histological, genotoxic, and biochemical effects on Cnesterodon decemmaculatus (Jenyns 1842) (Cyprinodontiformes, Poeciliidade): early response bioassays to assess the impact of receiving waters
title_full Histological, genotoxic, and biochemical effects on Cnesterodon decemmaculatus (Jenyns 1842) (Cyprinodontiformes, Poeciliidade): early response bioassays to assess the impact of receiving waters
title_fullStr Histological, genotoxic, and biochemical effects on Cnesterodon decemmaculatus (Jenyns 1842) (Cyprinodontiformes, Poeciliidade): early response bioassays to assess the impact of receiving waters
title_full_unstemmed Histological, genotoxic, and biochemical effects on Cnesterodon decemmaculatus (Jenyns 1842) (Cyprinodontiformes, Poeciliidade): early response bioassays to assess the impact of receiving waters
title_sort Histological, genotoxic, and biochemical effects on Cnesterodon decemmaculatus (Jenyns 1842) (Cyprinodontiformes, Poeciliidade): early response bioassays to assess the impact of receiving waters
dc.creator.none.fl_str_mv Ossana, Natalia Alejandra
Baudou, Federico Gastón
Castañé, Patricia M.
Tripoli, Luis
Soloneski, Sonia Maria Elsa
Ferrari, Lucrecia
author Ossana, Natalia Alejandra
author_facet Ossana, Natalia Alejandra
Baudou, Federico Gastón
Castañé, Patricia M.
Tripoli, Luis
Soloneski, Sonia Maria Elsa
Ferrari, Lucrecia
author_role author
author2 Baudou, Federico Gastón
Castañé, Patricia M.
Tripoli, Luis
Soloneski, Sonia Maria Elsa
Ferrari, Lucrecia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Cnesterodon decemmaculatus
receiving water assessment
comet assay
gills and liver alteration
oxidative stress
https://purl.org/becyt/ford/1.5
https://purl.org/becyt/ford/1
topic Cnesterodon decemmaculatus
receiving water assessment
comet assay
gills and liver alteration
oxidative stress
https://purl.org/becyt/ford/1.5
https://purl.org/becyt/ford/1
description In the present study, the toxicity of receiving waters from a highly polluted urban watercourse, the Reconquista River, Argentina, collected at a dam in the upstream part of the river was evaluated. Cnesterodon decemmaculatus, a widely distributed fish species in Pampasic rivers proposed for use in ecotoxicological evaluations, was used as a test organism. A 96-h acute toxicity bioassay with river water quality which has been characterized as moderately contaminated was performed. The treatment groups were (1) whole surface river water; (2) whole surface river water with 2 mg Cd/L added as a simulated metal contaminant pulse; (3) a negative control using reconstituted moderately hard water (MHW); (4) a metal positive control, MHW + 2 mg Cd/L; and (5) a positive genotoxicity control, MHW + 5 mg Cyclophosphamide/L (CP). The condition factor rate, micronuclei frequency, and comet assay from peripherical blood, structural changes of the gill arrangement by scanning electron microscope (SEM) analysis, histopathological changes in the liver and the glutathione-S-transferase, catalase, superoxide dismutase, glutathione, and protein content from the body midsection (viscera) were evaluated. According to our results, for short term exposure, SEM analyses of gills and liver histopathological analyses could be useful tools for the evaluation of target organ damage as well as comet assays for DNA damage. We propose that the 96-h laboratory bioassay protocol described is useful for monitoring the deterioration of water quality employing the teleost C. decemmaculatus and that the microscope analysis of gills and liver as well as the comet assay methodology could be sensitive endpoint indicators.
publishDate 2019
dc.date.none.fl_str_mv 2019-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/124023
Ossana, Natalia Alejandra; Baudou, Federico Gastón; Castañé, Patricia M.; Tripoli, Luis; Soloneski, Sonia Maria Elsa; et al.; Histological, genotoxic, and biochemical effects on Cnesterodon decemmaculatus (Jenyns 1842) (Cyprinodontiformes, Poeciliidade): early response bioassays to assess the impact of receiving waters; Hindawi Publishing Corporation; Journal of Toxicology; 2019; 1-2019; 1-14
1687-8191
1687-8205
CONICET Digital
CONICET
url http://hdl.handle.net/11336/124023
identifier_str_mv Ossana, Natalia Alejandra; Baudou, Federico Gastón; Castañé, Patricia M.; Tripoli, Luis; Soloneski, Sonia Maria Elsa; et al.; Histological, genotoxic, and biochemical effects on Cnesterodon decemmaculatus (Jenyns 1842) (Cyprinodontiformes, Poeciliidade): early response bioassays to assess the impact of receiving waters; Hindawi Publishing Corporation; Journal of Toxicology; 2019; 1-2019; 1-14
1687-8191
1687-8205
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1155/2019/4687685
info:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/jt/2019/4687685/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Hindawi Publishing Corporation
publisher.none.fl_str_mv Hindawi Publishing Corporation
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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spelling Histological, genotoxic, and biochemical effects on Cnesterodon decemmaculatus (Jenyns 1842) (Cyprinodontiformes, Poeciliidade): early response bioassays to assess the impact of receiving watersOssana, Natalia AlejandraBaudou, Federico GastónCastañé, Patricia M.Tripoli, LuisSoloneski, Sonia Maria ElsaFerrari, LucreciaCnesterodon decemmaculatusreceiving water assessmentcomet assaygills and liver alterationoxidative stresshttps://purl.org/becyt/ford/1.5https://purl.org/becyt/ford/1In the present study, the toxicity of receiving waters from a highly polluted urban watercourse, the Reconquista River, Argentina, collected at a dam in the upstream part of the river was evaluated. Cnesterodon decemmaculatus, a widely distributed fish species in Pampasic rivers proposed for use in ecotoxicological evaluations, was used as a test organism. A 96-h acute toxicity bioassay with river water quality which has been characterized as moderately contaminated was performed. The treatment groups were (1) whole surface river water; (2) whole surface river water with 2 mg Cd/L added as a simulated metal contaminant pulse; (3) a negative control using reconstituted moderately hard water (MHW); (4) a metal positive control, MHW + 2 mg Cd/L; and (5) a positive genotoxicity control, MHW + 5 mg Cyclophosphamide/L (CP). The condition factor rate, micronuclei frequency, and comet assay from peripherical blood, structural changes of the gill arrangement by scanning electron microscope (SEM) analysis, histopathological changes in the liver and the glutathione-S-transferase, catalase, superoxide dismutase, glutathione, and protein content from the body midsection (viscera) were evaluated. According to our results, for short term exposure, SEM analyses of gills and liver histopathological analyses could be useful tools for the evaluation of target organ damage as well as comet assays for DNA damage. We propose that the 96-h laboratory bioassay protocol described is useful for monitoring the deterioration of water quality employing the teleost C. decemmaculatus and that the microscope analysis of gills and liver as well as the comet assay methodology could be sensitive endpoint indicators.Fil: Ossana, Natalia Alejandra. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Programa de Ecofisiología Aplicada; Argentina. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; ArgentinaFil: Baudou, Federico Gastón. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Programa de Ecofisiología Aplicada; Argentina. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; ArgentinaFil: Castañé, Patricia M.. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Programa de Ecofisiología Aplicada; ArgentinaFil: Tripoli, Luis. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Programa de Ecofisiología Aplicada; ArgentinaFil: Soloneski, Sonia Maria Elsa. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ferrari, Lucrecia. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Programa de Ecofisiología Aplicada; ArgentinaHindawi Publishing Corporation2019-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/124023Ossana, Natalia Alejandra; Baudou, Federico Gastón; Castañé, Patricia M.; Tripoli, Luis; Soloneski, Sonia Maria Elsa; et al.; Histological, genotoxic, and biochemical effects on Cnesterodon decemmaculatus (Jenyns 1842) (Cyprinodontiformes, Poeciliidade): early response bioassays to assess the impact of receiving waters; Hindawi Publishing Corporation; Journal of Toxicology; 2019; 1-2019; 1-141687-81911687-8205CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1155/2019/4687685info:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/jt/2019/4687685/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:36:41Zoai:ri.conicet.gov.ar:11336/124023instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:36:41.374CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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