Obtaining sexual genotypes for breeding in buffel grass

Buffel grass (Cenchrus ciliaris L. syn. Pennisetum ciliare (L.) Link) is a species that is highly tolerant to drought and is used primarily as forage in drier regions throughout the subtropics and tropics. It reproduces mainly by apomixis and the acquisition of obligate sexual genotypes or facultati...

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Detalles Bibliográficos
Autores: Quiroga, Mariana Paola, Grunberg, Karina, Ribotta, Andrea Noemi, Lopez Colomba, Eliana, Carloni, Edgardo José, Tommasino, Exequiel Arturo, Luna, Celina Mercedes, Griffa, Sabrina Mariana
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2013
País:Argentina
Institución:Instituto Nacional de Tecnología Agropecuaria
Repositorio:INTA Digital (INTA)
Idioma:inglés
OAI Identifier:oai:localhost:20.500.12123/3710
Acceso en línea:https://www.sciencedirect.com/science/article/pii/S0254629913002986
http://hdl.handle.net/20.500.12123/3710
https://doi.org/10.1016/j.sajb.2013.04.016
Access Level:acceso abierto
Palabra clave:Cenchrus Ciliaris
Fitomejoramiento
Genotipos
Hibridación
Plant Breeding
Genotypes
Hybridization
Pasto buffel
Buffel Grass
Descripción
Sumario:Buffel grass (Cenchrus ciliaris L. syn. Pennisetum ciliare (L.) Link) is a species that is highly tolerant to drought and is used primarily as forage in drier regions throughout the subtropics and tropics. It reproduces mainly by apomixis and the acquisition of obligate sexual genotypes or facultative apomicts with high levels of sexuality is required for performing crosses and plant improvement. The aim of this study was to obtain sexual genotypes from controlled crosses using obligate apomictic cultivars and a sexual line. Twelve putative hybrid F1 plants were selected morphologically and two of them were identified as sexual genotypes by PCR using specific primers for reproductive mechanism. Cytoembryological analysis showed 65.5 and 71.3% meiotic embryo sacs in these plants and their hybrid nature was corroborated by AFLP. Both highly sexual genotypes could be used as female parents in crosses for obtaining improved cultivars of buffel grass.