Development of a biosensor for environmental monitoring based on microalgae immobilized in silica hydrogels
A new biosensor was designed for the assessment of aquatic environment quality. Three microalgae were used as toxicity bioindicators: Chlorella vulgaris, Pseudokirchneriella subcapitata and Chlamydomonas reinhardtii. These microalgae were immobilized in alginate and silica hydrogels in a two step pr...
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2012 |
| País: | Argentina |
| Institución: | Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales |
| Repositorio: | Biblioteca Digital (UBA-FCEN) |
| Idioma: | inglés |
| OAI Identifier: | paperaa:paper_14248220_v12_n12_p16879_Ferro |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_14248220_v12_n12_p16879_Ferro |
| Access Level: | acceso abierto |
| Palabra clave: | Algae Atrazine Biosensor Chlorophyll fluorescence DCMU Herbicides Sol-gel Aquatic environments Chlamydomonas reinhardtii Environmental Monitoring Fluorescence enhancement Pseudokirchneriella subcapitata Two-step procedure Hydrogels Microorganisms Silica Sol-gels Weed control Biosensors atrazine diuron fresh water article chemistry Chlorella vulgaris environmental monitoring equipment genetic procedures human hydrogel isolation and purification microalga water pollutant Biosensing Techniques Diuron Fresh Water Humans Hydrogel Microalgae Water Pollutants, Chemical |
| Sumario: | A new biosensor was designed for the assessment of aquatic environment quality. Three microalgae were used as toxicity bioindicators: Chlorella vulgaris, Pseudokirchneriella subcapitata and Chlamydomonas reinhardtii. These microalgae were immobilized in alginate and silica hydrogels in a two step procedure. After studying the growth rate of entrapped cells, chlorophyll fluorescence was measured after exposure to (3-(3,4-dichlorophenyl)-1,1-dimethylurea) (DCMU) and various concentrations of the common herbicide atrazine. Microalgae are very sensitive to herbicides and detection of fluorescence enhancement with very good efficiency was realized. The best detection limit was 0.1 μM, obtained with the strain C. reinhardtii after 40 minutes of exposure. © 2012 by the authors; licensee MDPI, Basel, Switzerland. |
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