Molecular identification for Helicoverpa spp. in Bt and non- Bt crops in central Argentina

The objective of this work was to identify, by molecular analysis, the species of the genus Helicoverpa in corn, soybean, and lucerne Bt and non-Bt crops, in central Argentina. A total of 1,343 carterpillars were analyzed by PCR-RFLP. Helicoverpa zea was the only species detected on non-Bt and on mo...

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Autores: Balbi, Emilia Ines, Flores, Fernando Miguel, Tosto, Daniela Sandra, Arneodo Larochette, Joel Demian
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:Argentina
Institución:Instituto Nacional de Tecnología Agropecuaria
Repositorio:INTA Digital (INTA)
Idioma:inglés
OAI Identifier:oai:localhost:20.500.12123/12261
Acceso en línea:http://hdl.handle.net/20.500.12123/12261
https://www.scielo.br/j/pab/a/yJWqGg7F6bXWZdMYn7S4NWN/
https://doi.org/10.1590/S1678-3921.pab2022.v57.02317
Access Level:acceso abierto
Palabra clave:Helicoverpa zea
Helicoverpa armigera
PCR
Control Biológico
RFLP
Argentina
Biological Control
Restriction Fragment Length Polymorphism
Helicoverpa gelotopoeon
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spelling Molecular identification for Helicoverpa spp. in Bt and non- Bt crops in central ArgentinaIdentificação molecular de Helicoverpa spp. em culturas Bt e não Bt no centro da ArgentinaBalbi, Emilia InesFlores, Fernando MiguelTosto, Daniela SandraArneodo Larochette, Joel DemianHelicoverpa zeaHelicoverpa armigeraPCRControl BiológicoRFLPArgentinaBiological ControlRestriction Fragment Length PolymorphismHelicoverpa gelotopoeonThe objective of this work was to identify, by molecular analysis, the species of the genus Helicoverpa in corn, soybean, and lucerne Bt and non-Bt crops, in central Argentina. A total of 1,343 carterpillars were analyzed by PCR-RFLP. Helicoverpa zea was the only species detected on non-Bt and on most Bt corn hybrids. The species H. gelotopoeon predominated on non-Bt soybean and on lucerne, and H. zea larvae occurred in low proportion. Bt soybean did not show Helicoverpa spp. infestations. The exotic H. armigera was not found, though adult moths of this species were light-trapped in a complementary sampling.O objetivo deste trabalho foi identificar, por análise molecular, as espécies do gênero Helicoverpa nas lavouras – Bt e não Bt – de milho, soja e alfafa, no centro da Argentina. Um total de 1.343 lagartas foram analisadas por PCR-RFLP. A espécie H. zea foi a única detectada no milho não Bt e na maioria do Bt. A espécie H. gelotopoeon predominou na soja não Bt e na alfafa, mas H. zea ocorreu em baixa proporção. A soja Bt não apresentou infestações por Helicoverpa spp. A exótica H. armigera não foi encontrada, porém, mariposas adultas dessa espécie foram capturadas com armadilha luminosa em uma amostragem complementar.Instituto de Microbiología y Zoología Agrícola (IMYZA)Fil: Balbi, Emilia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; ArgentinaFil: Flores, Fernando Miguel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; ArgentinaFil: Tosto, Daniela Sandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Arneodo Larochette, Joel Demian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaEmbrapa Secretaria de Pesquisa e Desenvolvimento2022-07-07T10:30:16Z2022-07-07T10:30:16Z2022-06-24info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/12261https://www.scielo.br/j/pab/a/yJWqGg7F6bXWZdMYn7S4NWN/1678-39210100-204Xhttps://doi.org/10.1590/S1678-3921.pab2022.v57.02317Pesquisa Agropecuária Brasileira 57 : e02317 (junio 2022)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2024-05-15T12:25:22Zoai:localhost:20.500.12123/12261instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2024-05-15 12:25:22.93INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Molecular identification for Helicoverpa spp. in Bt and non- Bt crops in central Argentina
Identificação molecular de Helicoverpa spp. em culturas Bt e não Bt no centro da Argentina
title Molecular identification for Helicoverpa spp. in Bt and non- Bt crops in central Argentina
spellingShingle Molecular identification for Helicoverpa spp. in Bt and non- Bt crops in central Argentina
Balbi, Emilia Ines
Helicoverpa zea
Helicoverpa armigera
PCR
Control Biológico
RFLP
Argentina
Biological Control
Restriction Fragment Length Polymorphism
Helicoverpa gelotopoeon
title_short Molecular identification for Helicoverpa spp. in Bt and non- Bt crops in central Argentina
title_full Molecular identification for Helicoverpa spp. in Bt and non- Bt crops in central Argentina
title_fullStr Molecular identification for Helicoverpa spp. in Bt and non- Bt crops in central Argentina
title_full_unstemmed Molecular identification for Helicoverpa spp. in Bt and non- Bt crops in central Argentina
title_sort Molecular identification for Helicoverpa spp. in Bt and non- Bt crops in central Argentina
dc.creator.none.fl_str_mv Balbi, Emilia Ines
Flores, Fernando Miguel
Tosto, Daniela Sandra
Arneodo Larochette, Joel Demian
author Balbi, Emilia Ines
author_facet Balbi, Emilia Ines
Flores, Fernando Miguel
Tosto, Daniela Sandra
Arneodo Larochette, Joel Demian
author_role author
author2 Flores, Fernando Miguel
Tosto, Daniela Sandra
Arneodo Larochette, Joel Demian
author2_role author
author
author
dc.subject.none.fl_str_mv Helicoverpa zea
Helicoverpa armigera
PCR
Control Biológico
RFLP
Argentina
Biological Control
Restriction Fragment Length Polymorphism
Helicoverpa gelotopoeon
topic Helicoverpa zea
Helicoverpa armigera
PCR
Control Biológico
RFLP
Argentina
Biological Control
Restriction Fragment Length Polymorphism
Helicoverpa gelotopoeon
description The objective of this work was to identify, by molecular analysis, the species of the genus Helicoverpa in corn, soybean, and lucerne Bt and non-Bt crops, in central Argentina. A total of 1,343 carterpillars were analyzed by PCR-RFLP. Helicoverpa zea was the only species detected on non-Bt and on most Bt corn hybrids. The species H. gelotopoeon predominated on non-Bt soybean and on lucerne, and H. zea larvae occurred in low proportion. Bt soybean did not show Helicoverpa spp. infestations. The exotic H. armigera was not found, though adult moths of this species were light-trapped in a complementary sampling.
publishDate 2022
dc.date.none.fl_str_mv 2022-07-07T10:30:16Z
2022-07-07T10:30:16Z
2022-06-24
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/12261
https://www.scielo.br/j/pab/a/yJWqGg7F6bXWZdMYn7S4NWN/
1678-3921
0100-204X
https://doi.org/10.1590/S1678-3921.pab2022.v57.02317
url http://hdl.handle.net/20.500.12123/12261
https://www.scielo.br/j/pab/a/yJWqGg7F6bXWZdMYn7S4NWN/
https://doi.org/10.1590/S1678-3921.pab2022.v57.02317
identifier_str_mv 1678-3921
0100-204X
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Embrapa Secretaria de Pesquisa e Desenvolvimento
publisher.none.fl_str_mv Embrapa Secretaria de Pesquisa e Desenvolvimento
dc.source.none.fl_str_mv Pesquisa Agropecuária Brasileira 57 : e02317 (junio 2022)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
instname_str Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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