Single-stranded oligodeoxynucleotides induce plant defence in Arabidopsis thaliana

Background and AimsSingle-stranded DNA oligodeoxynucleotides (ssODNs) have been shown to elicit immune responses in mammals. In plants, RNA and genomic DNA can activate immunity, although the exact mechanism through which they are sensed is not clear. The aim of this work was to study the possible e...

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Autores: Toum, Laila, Conti, Gabriela, Coppola Guerriero, Francesca Marina, Conforte, Valeria Paola, Garolla, Franco A., Asurmendi, Sebastian, Vojnov, Adrián Alberto, Gudesblat, Gustavo Eduardo
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/112505
Acceso en línea:http://hdl.handle.net/11336/112505
Access Level:acceso embargado
Palabra clave:PLANT IMMUNE SYSTEM
ELICITOR
BAK1
STOMATAL IMMUNITY
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
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oai_identifier_str oai:ri.conicet.gov.ar:11336/112505
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Single-stranded oligodeoxynucleotides induce plant defence in Arabidopsis thaliana
title Single-stranded oligodeoxynucleotides induce plant defence in Arabidopsis thaliana
spellingShingle Single-stranded oligodeoxynucleotides induce plant defence in Arabidopsis thaliana
Toum, Laila
PLANT IMMUNE SYSTEM
ELICITOR
BAK1
STOMATAL IMMUNITY
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short Single-stranded oligodeoxynucleotides induce plant defence in Arabidopsis thaliana
title_full Single-stranded oligodeoxynucleotides induce plant defence in Arabidopsis thaliana
title_fullStr Single-stranded oligodeoxynucleotides induce plant defence in Arabidopsis thaliana
title_full_unstemmed Single-stranded oligodeoxynucleotides induce plant defence in Arabidopsis thaliana
title_sort Single-stranded oligodeoxynucleotides induce plant defence in Arabidopsis thaliana
dc.creator.none.fl_str_mv Toum, Laila
Conti, Gabriela
Coppola Guerriero, Francesca Marina
Conforte, Valeria Paola
Garolla, Franco A.
Asurmendi, Sebastian
Vojnov, Adrián Alberto
Gudesblat, Gustavo Eduardo
author Toum, Laila
author_facet Toum, Laila
Conti, Gabriela
Coppola Guerriero, Francesca Marina
Conforte, Valeria Paola
Garolla, Franco A.
Asurmendi, Sebastian
Vojnov, Adrián Alberto
Gudesblat, Gustavo Eduardo
author_role author
author2 Conti, Gabriela
Coppola Guerriero, Francesca Marina
Conforte, Valeria Paola
Garolla, Franco A.
Asurmendi, Sebastian
Vojnov, Adrián Alberto
Gudesblat, Gustavo Eduardo
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv PLANT IMMUNE SYSTEM
ELICITOR
BAK1
STOMATAL IMMUNITY
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic PLANT IMMUNE SYSTEM
ELICITOR
BAK1
STOMATAL IMMUNITY
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description Background and AimsSingle-stranded DNA oligodeoxynucleotides (ssODNs) have been shown to elicit immune responses in mammals. In plants, RNA and genomic DNA can activate immunity, although the exact mechanism through which they are sensed is not clear. The aim of this work was to study the possible effect of ssODNs on plant immunity.Key ResultsThe ssODNs IMT504 and 2006 increased protection against the pathogens Pseudomonas syringae pv. tomato DC3000 and Botrytis cinerea but not against tobacco mosaic virus-Cg when infiltrated in Arabidopsis thaliana. In addition, ssODNs inhibited root growth and promoted stomatal closure in a concentration-dependent manner, with half-maximal effective concentrations between 0.79 and 2.06 µM. Promotion of stomatal closure by ssODNs was reduced by DNase I treatment. It was also diminished by the NADPH oxidase inhibitor diphenyleneiodonium and by coronatine, a bacterial toxin that inhibits NADPH oxidase-dependent reactive oxygen species (ROS) synthesis in guard cells. In addition it was found that ssODN-mediated stomatal closure was impaired in bak1-5, bak1-5/bkk1, mpk3 and npr1-3 mutants. ssODNs also induced early expression of MPK3, WRKY33, PROPEP1 and FRK1 genes involved in plant defence, an effect that was reduced in bak1-5 and bak1-5/bkk1 mutants.ConclusionsssODNs are capable of inducing protection against pathogens through the activation of defence genes and promotion of stomatal closure through a mechanism similar to that of other elicitors of plant immunity, which involves the BAK1 co-receptor, and ROS synthesis.
publishDate 2020
dc.date.none.fl_str_mv 2020-09
info:eu-repo/date/embargoEnd/2021-03-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/112505
Toum, Laila; Conti, Gabriela; Coppola Guerriero, Francesca Marina; Conforte, Valeria Paola; Garolla, Franco A.; et al.; Single-stranded oligodeoxynucleotides induce plant defence in Arabidopsis thaliana; Oxford University Press; Annals of Botany; 126º; 3; 9-2020; 413–422
0305-7364
CONICET Digital
CONICET
url http://hdl.handle.net/11336/112505
identifier_str_mv Toum, Laila; Conti, Gabriela; Coppola Guerriero, Francesca Marina; Conforte, Valeria Paola; Garolla, Franco A.; et al.; Single-stranded oligodeoxynucleotides induce plant defence in Arabidopsis thaliana; Oxford University Press; Annals of Botany; 126º; 3; 9-2020; 413–422
0305-7364
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/aob/advance-article/doi/10.1093/aob/mcaa061/5817573
info:eu-repo/semantics/altIdentifier/doi/10.1093/aob/mcaa061
dc.rights.none.fl_str_mv info:eu-repo/semantics/embargoedAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv embargoedAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799195896266620928
spelling Single-stranded oligodeoxynucleotides induce plant defence in Arabidopsis thalianaToum, LailaConti, GabrielaCoppola Guerriero, Francesca MarinaConforte, Valeria PaolaGarolla, Franco A.Asurmendi, SebastianVojnov, Adrián AlbertoGudesblat, Gustavo EduardoPLANT IMMUNE SYSTEMELICITORBAK1STOMATAL IMMUNITYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background and AimsSingle-stranded DNA oligodeoxynucleotides (ssODNs) have been shown to elicit immune responses in mammals. In plants, RNA and genomic DNA can activate immunity, although the exact mechanism through which they are sensed is not clear. The aim of this work was to study the possible effect of ssODNs on plant immunity.Key ResultsThe ssODNs IMT504 and 2006 increased protection against the pathogens Pseudomonas syringae pv. tomato DC3000 and Botrytis cinerea but not against tobacco mosaic virus-Cg when infiltrated in Arabidopsis thaliana. In addition, ssODNs inhibited root growth and promoted stomatal closure in a concentration-dependent manner, with half-maximal effective concentrations between 0.79 and 2.06 µM. Promotion of stomatal closure by ssODNs was reduced by DNase I treatment. It was also diminished by the NADPH oxidase inhibitor diphenyleneiodonium and by coronatine, a bacterial toxin that inhibits NADPH oxidase-dependent reactive oxygen species (ROS) synthesis in guard cells. In addition it was found that ssODN-mediated stomatal closure was impaired in bak1-5, bak1-5/bkk1, mpk3 and npr1-3 mutants. ssODNs also induced early expression of MPK3, WRKY33, PROPEP1 and FRK1 genes involved in plant defence, an effect that was reduced in bak1-5 and bak1-5/bkk1 mutants.ConclusionsssODNs are capable of inducing protection against pathogens through the activation of defence genes and promotion of stomatal closure through a mechanism similar to that of other elicitors of plant immunity, which involves the BAK1 co-receptor, and ROS synthesis.Fil: Toum, Laila. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein". Fundación Pablo Cassará. Instituto de Ciencia y Tecnología "Dr. César Milstein"; ArgentinaFil: Conti, Gabriela. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Coppola Guerriero, Francesca Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biociencias, Biotecnología y Biología Traslacional.; ArgentinaFil: Conforte, Valeria Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein". Fundación Pablo Cassará. Instituto de Ciencia y Tecnología "Dr. César Milstein"; ArgentinaFil: Garolla, Franco A.. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; ArgentinaFil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Vojnov, Adrián Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein". Fundación Pablo Cassará. Instituto de Ciencia y Tecnología "Dr. César Milstein"; ArgentinaFil: Gudesblat, Gustavo Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biociencias, Biotecnología y Biología Traslacional.; ArgentinaOxford University Press2020-09info:eu-repo/date/embargoEnd/2021-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/112505Toum, Laila; Conti, Gabriela; Coppola Guerriero, Francesca Marina; Conforte, Valeria Paola; Garolla, Franco A.; et al.; Single-stranded oligodeoxynucleotides induce plant defence in Arabidopsis thaliana; Oxford University Press; Annals of Botany; 126º; 3; 9-2020; 413–4220305-7364CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/aob/advance-article/doi/10.1093/aob/mcaa061/5817573info:eu-repo/semantics/altIdentifier/doi/10.1093/aob/mcaa061info:eu-repo/semantics/embargoedAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T14:07:32Zoai:ri.conicet.gov.ar:11336/112505instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 14:07:32.881CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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