Experimental and computational studies of the effects of free DHA on a model phosphatidylcholine membrane

Docosahexaenoic acid (DHA, 22:6) is a natural active compound that has raised considerable interest due to its several biological effects. In this work, effects of free DHA on the physicochemical properties of dipalmitoylphosphatidylcholine (DPPC) liposomes are investigated in terms of lipid membran...

Full description

Bibliographic Details
Authors: Verde, Alejandro Raúl, Sierra, Maria Belen, Alarcon, Laureano Martin, Pedroni, Viviana Isabel, Appignanesi, Gustavo Adrian, Morini, Marcela Ana
Format: article
Status:Published version
Publication Date:2018
Country:Argentina
Institution:Consejo Nacional de Investigaciones Científicas y Técnicas
Repository:CONICET Digital (CONICET)
Language:English
OAI Identifier:oai:ri.conicet.gov.ar:11336/91980
Online Access:http://hdl.handle.net/11336/91980
Access Level:Open access
Keyword:DHA
DPPC LIPOSOME
FLUIDITY
MEMBRANE STRUCTURE
MOLECULAR DYNAMICS SIMULATIONS
ZETA POTENTIAL
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
Description
Summary:Docosahexaenoic acid (DHA, 22:6) is a natural active compound that has raised considerable interest due to its several biological effects. In this work, effects of free DHA on the physicochemical properties of dipalmitoylphosphatidylcholine (DPPC) liposomes are investigated in terms of lipid membrane structure, by means of temperature-dependent zeta potential measurements, density studies and molecular dynamics simulations. Experimental results predict, in good agreement with simulations that DHA readily incorporates into DPPC liposomes, localizing at the lipid headgroup region. These data show that DHA induces changes in the lipid bilayer structure as well as in membrane fluidity.