Electrochemical determination of β-Lactoglobulin employing a polystyrene bead-modified carbon nanotube ink
In this article, we introduce the use of a carboxy-functionalized waterborne carbon nanotube ink for the fabrication of an amperometric biosensor aimed at the quantification of β-lactoglobulin. Detection of this protein from cow’s milk was performed by a sandwich immunoassay onto printed carbon nano...
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2018 |
| País: | Argentina |
| Institución: | Instituto Nacional de Tecnología Industrial |
| Repositorio: | Repositorio Institucional del Instituto Nacional de Tecnología Industrial (INTI) |
| Idioma: | inglés |
| OAI Identifier: | nuevadc:Molinari2018Electrochemical_pdf |
| Acceso en línea: | https://app.inti.gob.ar/greenstone3/sites/localsite/collect/nuevadc/index/assoc/Molinari/2018Elec.dir/doc.pdf |
| Access Level: | acceso abierto |
| Palabra clave: | Métodos electroquímicos Sensores electroquímicos Nanotubos de carbón Alérgenos Alimentos alergénicos Poliestireno Tintas Biosensores |
| Sumario: | In this article, we introduce the use of a carboxy-functionalized waterborne carbon nanotube ink for the fabrication of an amperometric biosensor aimed at the quantification of β-lactoglobulin. Detection of this protein from cow’s milk was performed by a sandwich immunoassay onto printed carbon nanotube electrodes. The electrodes were printed using a carbon nanotube ink modified with polystyrene beads containing a high amount of carboxylic groups for protein immobilization. This strategy showed enhanced sensing performance compared to the use of oxidative treatments for the functionalization of electrodes. These electrodes showed an excellent electrochemical behavior, and proteins could be immobilized on their surface via the carbodiimide reaction. These antibody-immobilized carbon nanotube electrodes allowed for the detection of β-lactoglobulin in sub-ppm concentrations. |
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