Characterization of lactobacilli strains derived from cocoa fermentation in the south of Bahia for the development of probiotic cultures

In the last years, several factors have contributed to the development of probiotic cultures from locally sourced strains. In this paper, we aimed to characterize Lactobacillus plantarum and Lactobacillus fermentum isolates derived from Brazilian cocoa fermentation for the development of new probiot...

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Detalhes bibliográficos
Autores: Teles Santos, Tiza, Santos Ornelas, Roberta María, Borges Arcucio, Leonardo, Messias Oliveira, Mayara, Nicoli, Jaques Robert, Villela Dias, Cristiano, Trovatti Uetanabaro, Ana Paula, Vinderola, Celso Gabriel
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:Argentina
Recursos:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/59370
Acesso em linha:http://hdl.handle.net/11336/59370
Access Level:acceso abierto
Palavra-chave:Probiotics
Chocolate
Isolation
https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
Descrição
Resumo:In the last years, several factors have contributed to the development of probiotic cultures from locally sourced strains. In this paper, we aimed to characterize Lactobacillus plantarum and Lactobacillus fermentum isolates derived from Brazilian cocoa fermentation for the development of new probiotic cultures. Isolates diversity was studied by RAPD and strains were further tested in vitro for their probiotic potential. Physiological traits such as heat tolerance, hydrophobicity, resistance to simulated gastrointestinal digestion and antibiotic susceptibility were studied. Besides, activity against food pathogens was tested through four different assays: deferred inhibition, co-aggregation, co-cultivation and antagonism of supernatants. Considering the resistance to simulated gastrointestinal digestion and the results from the antimicrobial and co-aggregation tests, L. plantarum 286 showed the most promising results, followed by L. plantarum 289, for further studies for their application as probiotics.