On-line microfluidic sensor integrated with an enzyme-modified pre-cell for the monitoring of paracetamol in pharmaceutical samples
An on-line microfluidic sensing device with an enzyme-modified pre-cell coupled to an amperometric detector for the monitoring of paracetamol in pharmaceutical formulations is described. Horseradish peroxidase (HRP) [EC 1.11.1.7], immobilized on a 3 μl pre-cell, in presence of hydrogen peroxide cata...
| Autores: | , , |
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| Tipo de documento: | artigo |
| Estado: | Versão publicada |
| Data de publicação: | 2006 |
| País: | Argentina |
| Recursos: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositório: | CONICET Digital (CONICET) |
| Idioma: | inglês |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/93548 |
| Acesso em linha: | http://hdl.handle.net/11336/93548 |
| Access Level: | Acceso aberto |
| Palavra-chave: | BIOSENSOR FIA GLASSY CARBON HORSERADISH PEROXIDASE PARACETAMOL https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
| Resumo: | An on-line microfluidic sensing device with an enzyme-modified pre-cell coupled to an amperometric detector for the monitoring of paracetamol in pharmaceutical formulations is described. Horseradish peroxidase (HRP) [EC 1.11.1.7], immobilized on a 3 μl pre-cell, in presence of hydrogen peroxide catalyses the oxidation of paracetamol to N-acetyl-p-benzoquinoneimine. The electrochemical reduction back to hydroquinone is detected on glassy carbon electrode surface at -0.10 V. The recovery of paracetamol from 10 samples ranged from 99.00 to 101.10%. This method could be used to determine paracetamol concentration in the range 0.35-100 μM (r = 0.997) with a limit of detection of 3.0 × 10-7 M and a relative standard deviation was less than 4.1% (n = 8). The method was successfully applied for the processing of as many as 20 samples per hour of paracetamol in pharmaceutical formulations. |
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